查询词典 vitro

This dissertation studied the isolation, purification, culture system optimization and transplantation techniques of goat spermatogonium in vitro respectively to construct an optimized long term culture system to facilitate in vitro manipulate studies for future.

十年来小鼠精原干细胞培养和移植探索的不断完善，为各种哺乳动物及人类精原干细胞培养体系的建立和移植实验提供了新的思路和技术平台。

This method is more convenient, rapid and economical than the traditional Sevag method. A standard strain of herpes simplex virus type 1(HSV-1, SM44 strain) was used to investigate the antiviral activities of PSP in vitro and in vivo. In vitro experiments were carried out in Vero cells, firstly the activity of PSP against HSV-1 was proved to be better than acidovir, which is the most widely used antiviral agent in clinic.

抗病毒作用的研究选用单纯疱疹病毒1型(HSV-1)标准株(SM44)，体外实验在Vero细胞中进行，在证实PSP抗HSV-1活性优于目前临床治疗该病毒感染的首选药物阿昔洛韦的基础上，进一步探讨了PSP抗HSV-1作用的机制。

In the present experiment, the screening techniques for salt tolerance and mutants of strawberry leaf and anther callus in vitro were studied, and primary screening system for salt tolerance evaluation was established. It was studied the sensitivity to mutagen of different strawberry cultivars and different tissues in vitro.

本试验以草莓离体叶片及花药愈伤组织为试材进行了耐盐突变体的诱变及筛选研究，初步建立了草莓耐盐突变体诱变筛选的技术体系，研究了草莓品种以及离体组织对诱变剂的敏感性，并且对不同诱变处理再生植株的耐盐性差异进行了比较。

Effects of gamma irradiation on shoot regeneration from leaf and bud growth in vitro of strawberry 1 The leaf was very sensitive to the gamma irradiation, and 'Toyonoka' was more sensitive than 'Akihime'. The LD50 (50% decrease of generation) of strawberry leaf was between 10 and 20 Gy. 2 Strawberry leaves were inoculated in vitro at 8 d-15 d after gamma rays irradiation, and the shoot regeneration rate from of 'Toyonoka' increased at 10 Gy and almost no difference from 20 Gy to 40 Gy. The positive effect dose for 'Akihime'.was from 10 Gy to 30 Gy, however, 40 Gy decreased shoot regeneration rate.

2.60Co γ辐射对不同品种草莓离体叶片再生及芽生长的影响 1）叶片对辐射敏感，叶片再生的半致死剂量为10 Gy-20 Gy，'丰香'品种比'章姬'品种敏感； 2）辐射后8 d-15 d接种，'丰香'叶片的不定芽再生率在剂量10 Gy呈上升趋势，而其他剂量则几乎未见有变化，'章姬'在剂量10 Gy-30 Gy，叶片的不定芽再生率提高较大，40 Gy仍表现轻微下降； 3）适宜的辐射剂量对草莓试管苗增殖有促进作用。

Transduction of TNF-α gene into TIL of human glioma by retroviral vectors and study of its biological activity in vitro. The development of retroviral vectors for TNF-α gene transfer and preparation of carrier cells-TIL have al- lowed transducing marked gene NeoR and purpose gene TNF-α into TIL cells respectively by use of monoclone tilter virus PLC-2 and PLJC-5. Detect the proliferation of TIL, expression of TNF-α and the anti-tumor activity of glio- ma TJ8510 cells in vitro.

在TNF-α逆转录病毒转移系统的建立及运载细胞TIL制备的基础上，利用构建的单克隆高滴度病毒细胞株PLC-2和PLJC-5将标记基因NcoR和目的基因TNF-α分别导入到TIL细胞中；对TNF-α基因转导后的TIL细胞进行细胞的增殖情况、TNF-α表达水平及对人脑胶质瘤TJ8510细胞的体外抗瘤活性的检测。

The transter and expression of endostatin gene was analyzed by PCR, the effects of inhibition on proliferation of endothelial cell by endostatin in vitro by MTT method, and the effects on the growth of LoVo、 SGC-7901 in vitro was surveyed, and the growth of expressing endostatin LoVo、SGC-7901 cell line_in subcutaneously of nude mice was also measured.

用MTT法检测内皮抑素对内皮细胞体外增殖的抑制作用，并观察其对LoVo细胞和SGC-7901细胞体外生长的影响，观察表达内皮抑素基因的LoVo和SGC-7901细胞在裸鼠皮下种植的生长。

The effect of chromium trichloride on miotic maturation and fertilization ability of mouse oocyte was studied by in vitro culture of mouse oocyte and in vitro fertilization.

采用小鼠卵母细胞体外培养，体外受精的方法研究了三氯化铬对小鼠卵母细胞成熟和受精能力的影响。

In this study, an assessment system of chemicals toxicity to hair in vitro was established by means of cultivating hair follicle of dorsal skin and vibrissa of SD rats in vitro in Williams'E media without serum.

本研究选用SD大鼠背部皮肤毛囊和触须毛囊，在Williams'E无血清培养基中进行体外游离培养，建立了体外毛发毒性评价系统。

To make the wapiti sperm capacitated in vitro, we selected and used the method of sperm-up. By making the rate of acrosome reaction as a main index, and the sperm energy, the survial time and the fertilizated rate of wapiti sperm and hamster mature oocytes as auxiliary indexes, it were carried out to compare the effects of wapiti sperm capacitation in vitro under different temperature, different density of pyruvate sodium, caffeine and heparin and different culture medium.

对马鹿精子，使用上浮法诱导其获能，通过对顶体染色测定精子的顶体反应率并作为主要指标，同时以精子活力、精子存活时间和异种受精率为辅助指标，对精子的获能效果进行评价，以比较不同培养温度、丙酮酸钠浓度、咖啡因浓度、肝素浓度以及培养基对马鹿精子体外获能效果的影响。

But the chondrocytes lost primary characteristics after several passages.Conclusion A great of chondrocytes can be got from xiphoid, and the chondrocytes can grow in suspension and keep primary characteristics for a long time when they are cultured with polyHEMA in vitro. In this way, good seed cells are provided for experiment in vitro, and for the study of tissue engineering, and for clinical study.

从剑突可获得大量软骨细胞，使用PolyHEMA试剂体外培养的软骨细胞，其软骨细胞能长期以悬浮状态生长，并能使软骨细胞长期保持原有的生物学特性，为体外实验及组织工程学的研究提供更优质种子细胞，为临床的进一步研究提供理论基础。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。