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MMP expression and activities were studied in macrophages after engulfment of apoptotic cholangiocytes in vitro.

我们在体外研究巨噬细胞吞噬凋亡胆管细胞后MMP的表达及活性变化。

In vitro assay showed that engulfment of apoptotic spermatogenic cells increases ATP production by Sertoli cells.

体外实验证实吞噬凋亡生精细胞能够促进Sertoli细胞产生ATP。

Five models were used to investigate the "Changdong"" s anti-tumor effect: mice S180 sarcoma solid model, mice liver cancer H22 solid model, mice Lewis lung cancer model, as well as human liver cancer QGY and colon cancer LOVO implanted in naked mice; mice liver cancer H22 was also used to evaluate the enhancive effect of "Changdong" when it was co-applied with other anti-tumor agent or with radiotherapy; The "Changdong"" s cytotoxic effect in vitro on such tumors as QGY, LOVO, lung cancer A549, breast cancer MCF-7, as well as mice leukemia P388 was observed through MTT method. And investigate the effect of "Changdong" on proliferation of spleen lymphocyte, on activity of NK cell and on synthesis of IL-2 in mice bearing Lewis lung cancer, respectively. With propidium iodide straining, cell aigptosis and cell circle were analyzed by flow cytometry. Result:"Changdong" has an evident tumor inhibitive effect on mice tumor model as well as human tumor implanted in naked mice with a quantity-effect relationship.

使用小鼠S180肉瘤、小鼠肝癌H22实体瘤和小鼠Lewis肺癌模型，以及人体肝癌QGY和人体肠癌LOVO裸鼠异种移植模型，进行"长动"的抗肿瘤药效学研究：用小鼠肝癌H22进行"长动"合并放疗和化疗的增效作用研究；用MTT法进行"长动"对肝癌QGY、肠癌LOVO、肺癌A549、乳腺癌MCF-7、小鼠白血病P388等10株人体和动物肿瘤细胞的体外细胞毒性作用研究；用淋巴细胞转化试验、小鼠NK细胞活性试验、小鼠胸腺细胞增殖法分别测定"长动"对荷Lewis肺癌小鼠的脾淋巴细胞增殖的影响、自然杀伤细胞活性的影响和对小鼠产生白介素-2(IL-2)的影响；用流式细胞仪PI单染色法进行"长动"对人体肝癌QGY细胞的体外诱导凋亡作用和对肿瘤细胞周期的影响的研究。

MethodsOf 47 rat OLTs, 20 were per-formed with conventional two-cuff technique, 27 were combined with external bile drain-age and enterostomy. In the experimental group,a self-made connector was used to rebuid in vitro bile flow bypass, and all rats were placed in self-made restraining cages for bile collection.

47例大鼠原位肝移植，其中27例采用改进术式：在传统肝移植胆道外引流的基础上，加行空肠造瘘，并在体外建立胆汁回流通路，术后通过自制的限制性大鼠笼固定大鼠以便采集胆汁；其余20例仅单纯行肝移植术。

Effects of in vitro maturation duration and enucleation time of buffalo oocytes on the NT embryo development were studied.

探讨了受体卵母细胞的成熟和去核时间对水牛核移植效果的影响。

The study aimed to provide references for the time of oocyte maturation in vitro and enucleation in the course of sheep nuclear transfer.

［目的］为绵羊克隆试验中卵母细胞的体外成熟及去核时间提供参考。

It was separated from rat's testis and identified by hematoxylin and eosin staining and immunocytochemical reaction, then cultivated in vitro.

从幼鼠睾丸中分离得到支持细胞，并通过苏木精-伊红和Fas-L免疫组化染色对分离得到的细胞进行了鉴定。

In vitro epicotyl explants from Jatropha curcas were cultured on MS medium with indole-3-butyric acid and 6-benzyladenine.

以麻疯树上胚轴为实验材料在MS添加IBA和BA的培养基上进行离体培养实验。

Result Based on directly regenerated plant of in vitro plant of Xinjiang cotton, the cotton epicotyl treated by pre-culture for 3-5 d was immersed in bacteria for 15 min and co-cultured for 2 d. Cephalothin at 100 mg/L was used to inhibit bacteria after 50 mg/L kan selection. And the combination of cytokinin with relatively high content and auxin with low content was used in its directly budding culture to obtain resistant seedlings.

结果］在新疆棉花离体培养植株直接再生植株基础上，经3~5d预培养处理过的棉花上胚轴，在浸菌15 min共培养2 d后，经50 mg/L kan筛选，用1000 mg/L头孢霉素抑菌，并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养，从而获得抗性小苗。

ResultBased on directly regenerated plant of in vitro plant of Xinjiang cotton,the cotton epicotyl treated by pre-culture for 3-5d was immersed in bacteria for 15min and co-cultured for 2d.Cephalothin at 1000mg/L was used to inhibit bacteria after 50mg/L kanselection.And the combination of cytokinin with relatively high content and auxin with lowcontent was used in its directly budding culture to obtain resistant seedlings.

结果在新疆棉花离体培养植株直接再生植株基础上，经3～5d预培养处理过的棉花上胚轴，在浸菌15min共培养2d后，经50mg/Lkan筛选，用1000mg/L头孢霉素抑菌，并用相对高含量细胞分裂素和低含量生长素的组合对其进行直接出芽培养，从而获得抗性小苗。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。