查询词典 vitro

Objective To study the occurence, development and regulation of reactive gliosis with astrocyte in vitro.

目的 研究体外星形胶质细胞反应性胶质化的发生、发展过程与规律。

Astrocyte Medium is a complete medium designed for optimal growth of normal human astrocytes in vitro.

星形胶质细胞培养基是专门为正常人类星形胶质细胞体外培养设计的最适于其生长的培养基。

Traumatic injury with secondary hypoxia insult can significantly increase the degree of injury of cultured astrocyte in vitro.

创伤后低氧可显著增加体外培养星形细胞的损伤程度，此模型可用于体外研究创伤后低氧二次打击的机制。

AIM: To observe whether hippocampal astrocyte conditioned medium can induce human adipose-derived adult stromal cells into neuron-like cells in vitro.

目的：观察海马区星形胶质细胞培养上清液能否在体外诱导人脂肪基质细胞向神经元样细胞分化。

The assumption behind the in vitro industry is that some people's genetic material is worth more than others' and deserves to be reproduced at any expense.

试管婴儿业背后有一个假设：有些人的基因比别人的基因有价值，值得不计成本来保存、延续。

Methods: A novel microwave antenna with water-cooled shaft was used in fresh liver of pig in vitro. Microwave energy was applied in one session at 60~90W for 5~25min, and the diameter and appearances of coagulation were observed.

采用一种新型的水冷式低杆温微波电极对离体新鲜猪肝行微波消融，单次能量输出60~90W，5~25min，观察不同消融条件造成的组织凝固范围和形态。

ATO can inhibit proliferation of MSC and promote its apoptosis in vitro.

ATO可在体外抑制MSC增殖，并促进其凋亡。

The Atropos Toolkit - The Atropos Toolkit allows experimentation with predictive capability inside a network while the network is operating: this might be best described as 'in vitro' prediction experimentation.

Atropos 工具集允许在运行的网络中进行预测能力测试。由于网络正在运行，这可以最恰当的描述为"试管"预测实验。

Objective: To express rALR in GS115 with the constructed pichia pastoris expression plasmid of rat augmenter of liver regeneration and purify it with ultrafiltration and identify its bioactivity in vitro, for studying its biological functions.

目的：利用已构建的大鼠肝再生增强因子毕赤酵母重组表达质粒pPIC9K-rALR，在酵母菌GS115中诱导表达rALR，并进行纯化和体外生物学活性鉴定，为进一步研究rALR的生物学功能提供基础。

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。