查询词典 vitro

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum. The acquired NASCs were subcultured for passage.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。

The prolif- erative ability of CD133 + tumor cells in vitro was estimated by MTT assay, and the proliferative ability of CD133 +, CD133 - and control SUNE cells were statistically compared. Finally, the immunocytochemical staining method and flow cytometry were used to detect differen- tial ability of CD133 + tumor cells in vitro .

采用免疫荧光细胞化学技术及流式细胞仪检测鼻咽癌细胞株SUNE中CD133的表达情况以及CD133+细胞体外分化能力；免疫磁珠分选技术纯化CD133+肿瘤细胞；MTT法检测CD133+细胞的体外增殖能力，并将其与CD133-及未分选细胞进行比较。

objective to investigate the existence of the erythropoiesis inhibitors.methods the present study included twelve undialyzed esrd patients with anemia.the sera from the uremic patients were added to cfu-e and bfu-e culture in the concentrations ranging from 1.25% to 5%.in vitro cfu-e and bfu-e growth in the presence of sera from esrd patients was compared with in the presence normal human subjects with the use of normal mice bone marrows.results the effects of the sera from uremic patients on cfu-e and bfu-e colony growth in concentrations ranging from 1.25% to 5% were investigated to be dose-dependent.the effect increased with the concentrations increasing of the sera（p＜0.05）.there were not significant inhibitory effect on the cfu-e and bfu-e growth of mice bone marrow in vitro from sera of the normal subjects.conclusion the erythropoiesis inhibitors exist in uremic sera which has a dose-dependent inhibitive effects on the growth cfu-e and bfu-e of normal mice bone marrow cells.

选择12例尿毒症肾性贫血未透析患者为试验组，另选正常健康体检者12例为正常对照组，将实验组、正常对照组血清分别稀释成不同终浓度，采用甲基纤维素细胞培养的方法对小鼠骨髓细胞进行培养，对比不同浓度血清组红系集落形成单位、红系爆式形成单位的集落数。结果在实验组中，与血清浓度为0的浓度组相比，与各浓度组血清共同培养的小鼠骨髓cfu-e和bfu-e的集落数均明显减少，且随血清浓度的增加，小鼠骨髓cfu-e和bfu-e的集落数逐渐减少，各浓度组间差异有显著性（p＜0.05）。结论尿毒症患者血清抑制小鼠骨髓红系祖细胞集落形成，随患者血清浓度的增加，抑制作用增强，具有剂量依赖性。

Methods Both in vitro and in vivo models of ventilator-induced lung injury were used. Alveolar epithelial cells were stretched in vitro to mimic the lung injury in VILI. Rats were ventilated at large tidal volume to produce ventilator-induced lung injury in vivo. A total of 23 inflammatory cytokines were screened with micro gene array in stretched alveolar epithelial cells.

方法通过呼吸机诱导肺损伤的体内及体外模型，将分离的肺泡上皮细胞于体外拉长，利用基因微阵列技术分析并筛选出上调的炎性细胞因子，之后给予大鼠呼吸机机械通气，留取血清标本，测量这些细胞因子在血清中是否上调。

Study on Antibacterial Effect in Vitro of Different Extract of Wikstroemia indica XIONG You-xiang, YOU Zhi-mian, CHENG Dong-qing, et alZhejiang University of TCM, Hangzhou 310053, China Abstract:Objective To research the antibacterial effect in vitro of different extract of Wikstroemia indica.

作者：熊友香，尤志勉，程东庆，姚振生作者单位：浙江中医药大学，浙江杭州 310053 目的比较了哥王不同提取部位的体外抑菌作用。

Methods: The bone marrow cells were obtained from long bone of new born Zelanian rabbits, the cells were isolated and cultured in vitro in conditioned medium, then the acquired cells which had propagated in vitro and undergone frozen storage and resuscitation and gelatin sponge were made into a complex.

取新生新西兰大白兔四肢长管骨骨髓，分离后加入条件培养液体外培养，冻存复苏的细胞以明胶海绵吸附后回植于异体兔的桡骨缺损处作为实验侧，对侧以明胶海绵吸附生理盐水作为对照侧。

DNA could be damnified by the NOCs in tap water directly, and the DNA adducts of such NOCs were formed in vitro and in vitro test.

东湖自来水中的NOCs有直接损伤生物体内外DNA、形成DNA加合物的作用。

By examining their expression during in vitro decidualization, we found that timing of the DAPs' expression in vitro was not consistent with it in vivo.

分析它们在体外蜕膜化过程中的表现情形，发现与在体内蜕膜化过程中的表现不一致。

Such studies are highly dependent on the efficient in vitro GLUT expression and, thus far, there has been no report of a system for high expression of GLUTs in vitro.

本实验利用分子生物学技术，获得了人类GLUT家族GLUT1、GLUT2和GLUT3三个家族成员的全长基因，构建了pESP-GLUTs表达载体，实现了GST-GLUT1、GST-GLUT2、GST-GLUT3融合蛋白在粟酒裂殖酵母中的高效表达。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。