查询词典 vitellin

The content variation to Chrysopa septempunctata"s vitellin was detected through ELISA. It indicated that vitellin was utilized gradually during embryonic development, and a fallowing trend in form of straightline was found in the whole embryonic development. Vitellin couldn"t be detected in just hatched larvae.

通过ELISA来测定大草蛉的胚胎发育和胚后发育中卵黄蛋白的含量变化，随着胚胎的发育，卵黄蛋白逐渐被利用，在整个胚胎发育过程中呈直线下降趋势，在刚孵化的幼虫中已检测不到卵黄蛋白的存在。

The variation to vitellin polypeptide was studied by SDS-PAGE during embryogenic and postembryonic development to Chrysopa septempunctata. The results indicated that vitellin was degradated constantly during embryonic development, and the bands of vitellin couldn't be detected in the 4th day of ovum development.

用SDS-PAGE研究了大草蛉胚胎发育和胚后发育过程中卵黄蛋白多肽的变化动态，结果表明，卵黄蛋白在胚胎发育过程中被不断降解，在卵发育的第4天已经基本上看不到卵黄蛋白的条带。

The main conclusions are as follows:The vitellin of Chrysopa septempunctata was extracted with 0.4mol/L NaCl and then precipitated in cold distilled water. Ion exchange chromatography and proteic gel recovery were used to purify it further. SDS-PAGE analysis shows that the vitellin is composed of two subunits, the large one 165kDa and the small one 46kDa.Then automatic amino acid analyzer was used to analyse amino acid composition of vitellin.

主要结论如下：用蒸馏水沉淀法对大草蛉的卵黄蛋白进行了初步纯化，而后又采用离子交换层析和蛋白胶回收的方法对卵黄蛋白进行了进一步的纯化；通过SDS-PAGE分析表明，大草蛉的卵黄蛋白由大165kDa和小46kDa两个亚基组成；用氨基酸自动分析仪对纯化后的卵黄蛋白进行了氨基酸组分分析。

A polyclonal antibody against vitellin was prepared in male rabbits and the specific antibody against vitellin was made through absorbtion by male worm homogenate. After purification, HRP was used to mark antibody, then ELISA was set up to detect vitellin content in the Chrysopa septempunctata fat-body, hemolymph and ovary.

提取了大草蛉的卵黄蛋白，免疫家兔，并将所得抗血清经雄虫匀浆液吸收，获得抗卵黄蛋白的特异抗体，经纯化后，用辣根过氧化物酶标记抗体，建立了ELISA法测定大草蛉脂肪体、血淋巴和卵巢中卵黄蛋白含量的方法。

This study aims at tick vitellogenesis and its mechanism. Six hybridoma cell lines secreting monoclonal antibody against vitellin of the hard tick Haemaphysalis longicornis were produced by fusing myeloma cells (SP2/0) with spleen cells, both from BALB/c mouse which were immunized with the Vn. They were named as 1B5, 2A7, 2B8, 2F2, 3A1 and 3G1, respectively. Further identification indicated that 1B5, 2B8 and 2F2 were of the isotype IgGA, 2A7 was of the isotype IgG1, while 3A1 and 3G1 were of the isotype IgG2a.

为研究蜱类的卵黄发生及其机理，用长角血蜱Haemaphysalis longicornis卵黄蛋白(vitellin，Vn)免疫BALB/c小鼠，取免疫鼠脾细胞与骨髓瘤细胞SP2/0进行融合，经3次克隆化筛选，获得6株能稳定分泌抗Vn的单克隆抗体，即1B5，2A7，2B8，2F2，3A1和3G1.1B5，2B8和2F2亚型为IgGA；2A7亚型为IgG1；3A1和3G1亚型为IgG2a.6株McAb均具有高度特异性，效价在1：105以上。

The characteristics of vitellin and its content in vitellogenesis were studied in Propylea japonica. The characterization of vitellin was carried out by PAGE and SDS-PAGE.

研究了龟纹瓢虫Propylea japonica卵黄蛋白的基本特性以及卵黄发生过程中卵黄蛋白的动态变化。

On the basis of purification and immunity of vitellin, antibodies of vitellin were obtained and used to measure the concentration of vitellin in embryo in different stages of embryonic development by enzyme-linked immunosorbent assay.

在采用纯化的卵黄磷蛋白免疫兔子而获得其抗体的基础上，运用酶联免疫吸附检测法(enZyme一linked ilnlnunosorbent assay，ELISA)分析测定了发育过程中胚胎内卵黄磷蛋白的含量。

He protein and vitellin content of the ovary in the TBTO groups showed similar changes in the RNA/DNA ratio of the ovary, indicating that the accumulation of TBTO in the ovary inhibited the synthetic activity of vitellin.

BTO暴露组的卵巢中蛋白质和卵黄蛋白含量显示了与RNA/DNA比相同的变化趋势，表明TBTO的积累阻碍卵黄蛋白的合成。

This paper was mainly working on the biochemical changes (water, carbohydrate, lipid, fatty acid, protein, amino acid) during embryonic development of Cherax quadricarinatus. At the same time, we especially worked on the yolk protein-vitellin in two aspects: 1. Isolation, purification and characterization of vitellin from the ovary of female Cherax quadricarinatus.

本文对红螯螯虾胚胎发育过程中的主要生化物质(水分、碳水化合物、脂类、脂肪酸、蛋白质、氨基酸)进行了测定，特别是对胚胎发育过程中主要的营养物质——卵黄磷蛋白的结构和含量变化做了细致的研究，同时讨论了这些物质的变化与红螯螯虾胚胎发育形态发生的相关性。

The soluble protein content decreased during embryonic development. Vitellin was a main component of soluble proteins. Besides, there were other soluble protein subunits such as 243 ku, 181 ku, 67 ku, 54 ku and 31 ku proteins.

可溶蛋白的含量在胚胎发育过程中逐渐降低；在成熟卵细胞和胚胎期，可溶蛋白在组成上以89ku和100ku的卵黄磷蛋白(Vitellin， Vn)为主，同时还存在243ku、181ku、67ku、54ku和31ku等其他一些蛋白亚基。40ku蛋白亚基仅出现在成熟卵细胞中，推测可能参与执行了特定的生殖功能。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。