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After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

After greater splanchnic nerve transection, changes of contractility of mesenteric micro-lymphatics in denervated rat were visualized and recorded by using a vital microscope with TV recorder during hemorrhagic shock and followed by refusion of withdrawn blood and infusion of saline.

为了探讨交感神经对失血性休克过程中淋巴微循环变化的影响，应用显微电视录象技术，观察了切断内脏大神经的大鼠在失血性休克及输血、补液过程中肠系膜微淋巴管收缩性的变化。

There always are not well visualized description of the existing Statistic Analysis systems. The main technology is using Excel to generate images and then copying to the web sites, or using specialistic statistic softwares.

现有的网上统计分析系统往往缺乏良好的可视化描述，使用的技术主要是由Excel生成图片粘切到网页或者使用专业统计软件。

Says Sridhar Marri, head of the communication design group at Infosys Technologies,"The design-led economic transformation that we have always dreamed of in India can now be visualized on the horizon."

Infosys 科技的沟通设计组首长 Sridhar Marri 说道。&在印度我们一直梦想的设计导向的经济转变，现在在水平线上可以看到了&

B3The sterically allowed values of φandψare visualized in a Ramachandran plot.

在莱幔图谱上可看见φ与ψ的立体允许值。

It is a great contrast that Greeks visualized the wild spirits and old gods as theriomorphic, grotesque and terrific fi...

希腊人将兽形的、怪诞的、恐怖的形象留给了代表原始的、无序的、未开化的、非理性的、象征自然蛮力的自然界精怪和老一辈神祗，而将美好的人类形象留给了象征理性、文明、和谐和宇宙新秩序的奥林波斯天神以及象征自然界温和力量的自然神女们，两者形成鲜明对比。

Clotted hemothorax was visualized on the computed tomography scan, then video-assistant thoracoscope was used for early evacuation of clotted hemothorax on the 14th day.

结论充分的术前准备和正确的围术期处理是肺移植成功的关键，重点是防治移植肺的早期失功能和围术期感染，同时早期监测急性排斥反应。

Methods One hundred thirty PCOS women and one hundred seventy five normal women as controls were enrolled in this study. The genotypes were screened by polymerase chain reaction-On/off switch and the product was isolated by electrophoresis on a 2.5% agarose gel containing ethidium bromide and visualized using an ultraviolet transilluminator.

应用On/off Switch-聚合酶链反应及琼脂糖凝胶电泳分离技术分析130例多囊卵巢综合征患者及175例对照组妇女TNF-α基因rs3179060C/A多态，比较rs3179060C/A两组等位基因及基因型与PCOS及其高雄激素之间的关系。

Once the hernia defects are visualized, a polypropylene or expanded polytetrafluoroethylene biomaterial is then inserted and secured to the transversalis fascia and/or Cooper's ligament with tacks or staples. The material is of a size that completely covers the myopectineal orifice.

一旦缺损区出现于视野中，送入聚丙烯或可延伸聚四氟乙烯网状补片，并将其固定于腹横筋膜上和/或Cooper韧带上，补片的大小以完全覆盖住内环口为宜。

On the front panel there is a display where the main operating parameters can be visualized; through the keyboard located below the display some settings can be modified underpower alarm threshold, output power indication, remote control selection, etc.

在前面板有一个显示器，主操作参数可以直观显示；通过安置在显示器下面的主板，一些设置可以更改（低电压报警极限，输出电力指示，遥控控制选择，等等

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。