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The glucoamylase I gene was inserted into the pAC1 plasmid,and a expression plasmid was constructed,then it was used to transform the yeast strain, S.cerevisiae AS2.1364,without auxtrophic marker,The amylolytic tests showed that when this transformant was incubated on plate of YPDS medium containing 1% glucose and 1% starch at 30℃ for 48 hrs zones of starch degradation could be visualized by stained with iodine vapour.

这说明酵母转化子不仅表达了该酶，且有效地分泌到胞外，从而水解培养基中的淀粉，进一步测定酵母转化子48hrs发酵液的葡萄糖淀粉酶活力为8.4u/ml，培养基中的淀粉已完全水解，其生物产量较高。

System computes combined navigation system of GPS/IMU, VECTOR and ascendable geometry stable platform, proceeds in space registration and time synchronization, calculates the systems accurate space, time, stance and course. Planar laser scanning data was filtered, registered space-wisely and time synchronously and fuse computered with other sensors data, extracted to 3D solid model of characteristic information of the road and outside of street, continuously extracted to point, line and plane, reconstructed combined with CCD camera data and visualized to 3D model true scene, finally implement acquisition and process of 3D space data timely, initiatively and integrally.

该套系统通过对GPS/IMU组合系统导航解算、VECTOR和升降几何平台进行空间配准、时间同步处理，计算出系统精确可靠的空间时间和姿态航向信息，二维激光扫描仪数据经过滤波去噪、和其他传感器数据融合、三维空间计算，解算出道路和两侧街景的三维空间数据模型，对空间激光数据模型提取点线面特征值，结合CCD相机数据进行立体重建和可视化，实现了实时、主动、完整地获取和处理三维空间数据信息。

System computes combined navigation system of GPS/IMU, VECTOR and ascendable geometry stable platform, proceeds in space registration and time synchronization, calculates the system??s accurate space, time, stance and course. Planar laser scanning data was filtered, registered space-wisely and time synchronously and fuse computered with other sensor??s data, extracted to 3D solid model of characteristic information of the road and outside of street, continuously extracted to point, line and plane, reconstructed combined with CCD camera data and visualized to 3D model true scene, finally implement acquisition and process of 3D space data timely, initiatively and integrally.

该套系统通过对GPS/IMU组合系统导航解算、VECTOR和升降几何平台进行空间配准、时间同步处理，计算出系统精确可靠的空间时间和姿态航向信息，二维激光扫描仪数据经过滤波去噪、和其他传感器数据融合、三维空间计算，解算出道路和两侧街景的三维空间数据模型，对空间激光数据模型提取点线面特征值，结合CCD相机数据进行立体重建和可视化，实现了实时、主动、完整地获取和处理三维空间数据信息。

Results: One case of type A1 (type Weinberg-A), one case of type A2 (type Weinberg-B) and one case of type A3 (abnormal origin of brachiocephalic artery) could be visualized clearly and directly by MSCTA. Morphology of PFAA and its flow disturbance could be seen on echocardiogram in cases of type Al and type A2, although the other case of type A3 was miss diagnosed due to ignoring the suprasternal views.

结果：3例PFAA包括新分型A1型（Weinberg-A型）、新分型A2型（Weinberg-B型）和A3型各1例，螺旋CT血管造影可清晰显示3例PFAA的空间结构及病理解剖，超声心动图能显示2例A1型和A2型的结构及血流动力学变化，1例A3型超声漏诊。

The hepatic metabolism is visualized by imaging sequential uptake of 6-carboxyfluorescein diacetate from hepatocytes and its excretion into bile canaliculi.

藉由观察6-CFDA在肝细胞内被转换并经由微胆管排出，我们真正看到肝脏的代谢功能。

An otherwise healthy 12-month-old girl presented for evaluation of reduced abduction of the left eye detected at 6 months of age. The remainder of the examination was unremarkable. A special MRI sequence-fast imaging employing steady-state acquisition-visualized the right but not the left sixth nerve cisternal segment. This is the first reported use of the MRI FIESTA sequence to diagnose aplasia of the sixth cranial nerve.

应用快速平衡稳态取像核磁技术检测第六颅神经发育不全：本研究首次报道利用 MRI FIESTA 诊断第六颅神经发育不全，一例12个月的婴儿其他各项指标正常，在6个月时发现左眼诱导注视减少，其他的检查未见明显异常，利用 MRI FIESTA 可检测到右侧第六颅神经，但左侧部分检测不到。

By setting two ROI, the main spinal cord fiber tracts, such as corticospinal or spinothalamic tract, were visualized.

结果 在DTT彩色编码图上，正常脊髓纤维束为头尾向的一束，显示了头尾侧的各向异性。

He had an uneventful postoperative recovery. Elderly patients who have emphysematous cholecystitis may present with only right upper abdominal pain. Other signs may be absent. Preoperative diagnosis can be difficult. When the gallbladder is poorly visualized and gas is suspected on ultrasonography, it should be defined by plain film and computed tomography and emergent cholecystectomy is needed to minimize the risk of mortality.

急性气肿性胆囊炎的老年患者，经常只以右上腹胀痛表现而缺乏典型的症候，术前诊断不容易，当超音波检查发现胆囊无法清楚显现而怀疑胆囊内有气体存在时，必须安排腹部X光及电脑断层作更进一步确认，一旦确立诊断，必须作紧急手术治疗以降低死亡的危险性。

This vein is easily visualized early in retropubic operations and has communicating branches over the bladder itself and into the endopelvic fascia.

在经耻骨后入路时，该静脉能被清晰看见，在膀胱表面形成一些交通支，然后进入盆内静脉。

In the A and B figures, small grape-like tissue covered over all the epiglottic area that is hypertrophic lymphoid tonsil. More advanced the fiberoptic laryngoscope during spontaneous inspiration phase, larynx and vocal cord can be visualized in the C and D figures. We must emphasize that endotracheal intubation and even artificial ventilation was hardly performed under direct laryngocopy and general anesthesia.

图片A和B中，覆盖整个会厌部的葡萄息肉状物就是濔漫增生的舌底部淋巴组织增生，当光纤内视镜随著病人自主呼吸向内向下移动，吐气时可以见到图片C和D中喉头及声带的开合，由於在全身麻醉下无自主呼吸时此病人咽喉部位的似帘幕状遮蔽特殊结构造成之困难插管甚至是困难通气。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。