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The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2/6/7病毒样颗粒,待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解,培养基上清和细胞裂解液样品两次40%蔗糖垫93000g超速离心,沉淀样品经SDS-PAGE胶分离,用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性,电镜检测重组病毒样颗粒的形态结构。

Three viruses?- But what's weirder, the healthy kid we tested

3种病毒?-但奇怪的是,健康婴儿的测试结果也是

Pigs have infected people with mixed-up viruses before, even triple whammies made of pig, bird and human flu genes, Richts paper shows.

里希特的论文表明,猪早就把混合病毒传染给了人类,甚至用猪、禽和人类病毒基因的三重混合沉重地打击了人类。

In fact, different mutations were found among viruses in various organs in the same individual.

事实上,在不同的基因突变被发现之间的病毒在各机关在同一个人。

Bath air forest fungicides present only as a raw material for plants to the air microbicides, will become of these bacteria, viruses, the nemesis, effectively preventing groups of cross-infection, especially for children, the elderly cross-infection.

森林浴空气杀菌剂做为目前唯一的一种以植物药为原料的空气杀菌剂,将成为这些细菌,病毒的克星,有效预防群体的交叉感染,特别是对老人儿童的交叉感染。

That is because the viruses float in the air in little respiratory droplets, Dr.

Palese博士认为这是因为病毒悬浮于少量的空气粒子中。

Compared with other potyviruses, there is the highest identity between WMV-CHN with Soybean mosaic virus, the nucleotide and amino acid identity is 78.4% and 86.1%, respectively; the second are Peanut stripw virus, Bean common mosaic necrosis virus, Bean common mosaic viurs, Blackeye cowpea mosaic viurs and Zucchini yellow mosaic virus, the homologies of nucleotide and amino acid are 68.3% and 70.9%, 67.2% and 71.2%, 67.3% and 70.3%, 67.7% and 70.9%, 62.2% and 62.9% in turn, respectively. The 5′-termini of WMV is intimately correlated with evolution of viruses.

与其他Potyvirus属成员相比,同源性最高的是大豆花叶病毒,核苷酸和氨基酸同源性分别为78.4%和86.1%,其他依次是花生条纹病毒病毒、菜豆普通花叶坏死病毒、菜豆普通花叶病毒、黑眼豇豆花叶病毒和小西葫芦黄花叶病毒,核苷酸和氨基酸同源性分别为68.3%和70.9%,67.2%和71.2%,67.3%和70.3%,67.7%和70.9%,62.2%和62.9%。

I imagine there might be a world of mutating buildings, living silicon polymers, software programs evolving offline, adaptable cars, rooms stuffed with coevolutionary furniture, gnatbots for cleaning, manufactured biological viruses that cure your illnesses, neural jacks, cyborgian body parts, designer food crops, simulated personalities, and a vast ecology of computing devices in constant flux.

没准儿我们将面临一个世界,其中有变异建筑,活生生的硅聚合物,软件程序脱机进化,还有可修改的车辆、塞满共同进化的家具的房间,打扫卫生的蚊型机器人,能治病的人造生物病毒,神经性插座,生控体系统的身体部件,带标签的粮食作物,模拟个性,以及由不断变化的计算机设备组成的巨型生态。

I imagine there might be a world of mutating buildings, living silicon polymers, software programs evolving offline, adaptable cars, rooms stuffed with coevolutionary furniture, gnatbots for cleaning, manufactured biological viruses that cure your illnesses, neural jacks, cyborgian body parts, designer food crops, simulated personalities, and a vast ecology of computing devices in constant flux.

没准儿我们将面临一个世界,它变异建筑林立,活生生的硅聚合物肆虐,软件程序脱机进化,还有可变车辆、房间,里面塞满共同进化的家具,打扫卫生的蚊型机器人,批量生产的用以治病的生物病毒,神经外衣,生控体系统的身体部件,特制粮食作物,模拟个性,以及不断变化的巨型生态学的计算机装置。

These same viruses and bacteria can also inhabit faucet handles, drinking fountains and school desks.

同样,这些病毒和细菌也可以居住在水龙头手柄,饮水机和课桌上。

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推荐网络例句

Recall that, in the Kolmogorov axiomatization, measurable sets are the sets which have a probability or, in other words, the sets corresponding to yes/no questions that have a probabilistic answer.

延期开工的Kolmogorov宣布要衡量所有套职能有限许多坐标限制在于衡量子寅。换句话说,如果是/否的问题回答f可看的价值观念在许多最有限坐标,然后它有一个概率的答案。

The experimental results showed that the algorithm can filter singular points and remain fixity of the target edge.

实验表明:基于模糊理论的图像智能滤波方法比常规图像滤波方法运算量小,它既能去除奇异点,又能保持图像中目标的边缘不变性,具有很好的滤波效果。

It explains in detail how the methods of elicitation, discussion, and induction are to be used in the physics teaching.

本文根据心理学原理论述了改革初中物理教学的必要性,并对"启发、讨论、归纳法"在物理教学中的应用作了详细的阐述。