查询词典 virus

In order to improve the viral detection method in shrimp, a more rapid and convenient multiplex reverse transcription-polymerase chain reaction was established in the present study. The new method was used for detection of shrimp viruses, including Yellow-head virus, white spot syndrome virus, Taura syndrome virus, hepatopancreatic parvovirus, infectious hypodermal and hematopoietic necrosis virus and monodon baculovirus.

为了提高对虾病毒检测的快速性和简便性，建立了一种可同时检测6种对虾病毒的RT-PCR方法，并利用该方法对来自广西沿海地区的130份病虾样品中的黄头病毒、白斑综合征病毒、桃拉病毒、肝胰腺坏死病毒、传染性皮下组织坏死病毒以及杆状病毒进行了检测。

Choose medicaments correctly, reasonable use medicaments, in order to make sure with medicine safety is mixed effective will be henceforth the main task that clinical medicine learns, for solution control hospital the clinical current situation that use drug reachs existence issue, promote the development of the reasonable job that use drug, raise a hospital reasonable the level that uses drug, the prescription of two place has outpatient service of author general hospital, be in hospital analytic research, summary goes out unreasonable a few big states that use drug, undertake classified at the same time. 1 clinical unreasonable with medical analysis each division all applies 1 abuse of 1 · or antibiotic of blind application antibiotic extensively, utilization rate amounts to 70% above, among them couplet is used rate be as high as 40%, appear even 2 couplet, triplex above, the commonnest abuse is infection of the upper respiratory tract, be admitted to hospital give 2~3 to plant antibiotic cure. 1 · of 1 · 1 without point to this kind to the cure that ask for uses drug unreasonable with medicine main show is in the person that the virus sex appeal such as hepatitis of sex of common cold, chicken pox, berpes zoster, virus is caught, in infection of not amalgamative bacterium, point to without what apply antibiotic ask for next using medicine. Incontrollable virus affects antibiotic, increase hepatic burden instead to patient of virus sex hepatitis.

梗概： 正确选择药物，并合理使用药物，以保证用药平安和有效是今后临床药学的重要任务，为了解把握医院临床用药目前状况及存在新问题，推动合理用药工作的发展，提高医院合理用药的水平，笔者将医院门诊、住院两处的处方进行分析探究，总结出不合理用药的几大状况，同时进行分类。1临床不合理用药的分析1·1滥用或盲目应用抗生素抗生素各科均广泛应用，使用率达70%以上，其中联用率高达40%，甚至出现二联、三联以上，最常见滥用为上呼吸道感染，入院给予2~3种抗生素治疗。1·1·1无指征的治疗用药这类不合理用药主要表现在普通感冒、水痘、带状疱疹、病毒性肝炎等病毒性感染者，在未合并细菌感染，无应用抗生素的指征下用药。

Meanwhile many cases were diagnosed to be sero positive of anti dengue virus. Dengue I virus were also isolated from five cases. RT PCR test also indicated 4 out of 8 samples contained dengue I virus, which was the same type of dengue virus found in the epidemic areas in Guangzhou at the same time of outbreak.

进一步的调查在病例的血标本中分离出5株登革I型病毒，同时用RT—PCR的方法从8份标本中检出4份登革I型病毒阳性，与同期广州市登革热流行的病毒型别一致；取患者的恢复期血清作肥达氏反应，结果抗&O&、&H&的滴度与第一份血相比，均显著下降。

Results The concentration of Chlorogenic acid was 0.05mg/ml, 0.1 mg/ml,0.4mg/ml,0.8mg/ml respectively,it can respectively inhibit respiratory syncytia virus,Coxsackie B 3 virus,adeno associated 7 virus,adeno associated 3 virus and Coxsackie B 5 vi...

结果 氯原酸浓度分别为 0 。0 5mg/ml、0 。1mg/ml、0 。4mg/ml、0 。8mg/ml时，可分别体外抑制合胞病毒、柯萨奇B3、腺病毒 7型、腺病毒 3型和柯萨奇B5型。结论氯原酸对常见呼吸道病毒有较强的抑制作用

Results After six passages in BHK-21 cells, cytopathic effect was observed by microscopy. The rescued virus was rejuvenated once in unweaned mice, and then came back to cell passage. We found that the poly tract of rescued virus was shortened when the virus was passaged twice in BHK-21 cells. Although the data of LD50 in mice and TCID50 in BHK-21 cells showed that the virulence and infectivity of genetic engineering viruses were lower than its parental virus, no significant difference was observed between the genetic engineering viruses with the different length poly tract.

我们发现，基因工程毒在BHK-21上连续传代至第6代时，可见明显的致细胞病变效应(cytopathic effect，CPE)；在经过一代乳鼠接种之后再重新适应到BHK-21细胞的过程中，poly区段出现了缩短现象；乳鼠致病性试验和BHK-21细胞TCID50测定结果表明，尽管基因工程毒与母本毒相比毒力偏低，但含有不同长度poly的基因工程毒之间并无显著差异。

In clean diluent condition, the median lethal concentration (MLC50) of CIO2 against bacteria and yeast was 9.4 and 8.4 ppm, respectively. In dirty diluent condition, the MLC50 of CIO2 against bacteria and yeasts was 39.9 and 24.3 ppm, respectively. The virucidal activity of CIO2 against 100 median embryo lethal dose (100 ELD50) of Newcastle disease virus, avian influenza virus, infectious bronchitis virus and infectious bursal disease virus was 1.2, 1.2, 2.5 and 1.2 ppm, respectively.

发现CIO2在乾净稀释液中对细菌和酵母菌的中间致死浓度(MLC50)分别为9.4和8.4ppm，CIO2在污浊稀释液中对细菌和酵母菌的MLC50分别为39.9和24.3ppm，CIO2之杀灭新城病病毒、H6N1型家禽流行性感冒病毒、传染性支气管炎病毒和传染性华氏囊炎病毒的浓度分别为1.2、1.2、2.5和1.2ppm。

Compared with other potyviruses, there is the highest identity between WMV-CHN with Soybean mosaic virus, the nucleotide and amino acid identity is 78.4% and 86.1%, respectively; the second are Peanut stripw virus, Bean common mosaic necrosis virus, Bean common mosaic viurs, Blackeye cowpea mosaic viurs and Zucchini yellow mosaic virus, the homologies of nucleotide and amino acid are 68.3% and 70.9%, 67.2% and 71.2%, 67.3% and 70.3%, 67.7% and 70.9%, 62.2% and 62.9% in turn, respectively. The 5′-termini of WMV is intimately correlated with evolution of viruses.

与其他Potyvirus属成员相比，同源性最高的是大豆花叶病毒，核苷酸和氨基酸同源性分别为78.4%和86.1%，其他依次是花生条纹病毒病毒、菜豆普通花叶坏死病毒、菜豆普通花叶病毒、黑眼豇豆花叶病毒和小西葫芦黄花叶病毒，核苷酸和氨基酸同源性分别为68.3%和70.9%，67.2%和71.2%，67.3%和70.3%，67.7%和70.9%，62.2%和62.9%。

A 1.8 kb fragment of 3' terminus of the viral RNA was amplified by RT PCR, cloned and its sequence was determined. Sequence comparisons showed that it shared 71.5%～99.1% homology with isolates of sugarcane mosaic virus, 67.8%～68.5% with sorghum mosaic virus and 38.4%～48.4% with maize dwarf mosaic virus, indicating that the pathogen of this disease on maize in Hangzhou was sugarcane mosaic virus.

病毒RNA13'端序列(1.8kb)与甘蔗花叶病毒同源性最高，达 71.5 %～ 99.1%，与高梁花叶病毒同源性次之，为 6 7.8%～ 6 8.5 %，与玉米矮花叶病毒同源性最低，仅为 38.4%～ 48.4%，从而初步认为此病害由SCMV引起。

Based on the conserved region nucleotide sequences of four viruses (Alfalfa Mosaic Virus, ALMV; Cucumber mosaic virus, CMV; Cucumber mosaic virus-satellite, CMV-sat; Potato virus Y, PVY) and one viroid (Potato spindle tuber viroid, PSTVd) which could be pathogenic on potato world and one inner control(18S rRNA), specific oligonucleotide probes and primers were designed and spotting for hybridization detection.

利用芯片点样仪将5种侵染马铃薯的病毒/类病毒(苜蓿花叶病毒、黄瓜花叶病毒、黄瓜花叶病毒-卫星病毒、马铃薯病毒Y、马铃薯块茎纺锤状类病毒)的保守区寡核苷酸(Oligonucleotide，oligo)探针和PCR探针点样于玻片，并以植物18S rRNA作为内参照制成基因芯片。

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根据监测和技术分析，瑞星安全专家认为，&磁碟机&病毒是一个具有明确经济目的的病毒犯罪团伙所为，他们借鉴了已被逮捕的&熊猫烧香&病毒团伙的犯罪经验，非常狡猾，在病毒制造、传播和躲避侦查等方面下足功夫。

You can do some assertiveness training.

你可以进行一些自信训练。

We were well on the path to making a rear-wheel-drive global platform," says Mays."

我们正致力于建立一个后轮驱动的平台，"Mays这样说道。"