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Recombinant plasmid pSVH 7 DNA of avian influenza virus H7 subtype heamagglutinin gene was encapsulated with DC-chol/DOPE liposomes and PC/chol/SA liposomes separately. Two-week old SPF chickens were intramuscularly inoculated with 50 μ g/0.2ml of the liposome entrapped PSVH 7 DNA. Four-weeks later, each chicken was challenged with 0.1ml 〓 AIV . One week after the challenge, the secretion of the cloacas was collected and transfected to chicken embryos to isolate the virus. The virus was isolated from 6/6 of the control group, 1/6 of the naked DNA group, 1/6 of the PC/chol/SA entrapped DNA group and 0/6 of the DC-chol/DOPE liposome entrapped group. The HI antibody titers (log2) of the four groups were 6. 83±0.98, 7. 0±1. 26, 7. 83±1. 17 and 8. 00±0.89 respectively 1-week after challenge, and 8. 5±0.55, 8. 17±0.82, 8. 68±0.45 and 9. 33±0.54 respectively 2-week after challenge. The results showed that inoculation of liposome entrapped DNA significantly enhanced resistance to virosis in animals.

将含禽流感病毒H7亚型血凝素基因的重组质粒pSVH7用DC-chol阳离子脂质体和胆固醇/卵磷脂/十八胺脂质体包裹,免疫2周龄SPF鸡,4周后用同型禽流感病毒进行人工感染,1周后采集泄殖腔分泌物分离病毒,结果未免疫组6/6分离到病毒,裸质粒DNA免疫组1/6分离到病毒胆固醇/卵磷脂/十八胺脂质体包裹DNA免疫组1/6分离到病毒,DC-chol脂质体DNA免疫组没有分离到病毒(0/6):人工感染后1周各组的HI抗体(Log2)分别为6.38±0.98,7.00±1.26,7.83±1.17,8.00±0.89,2周后为8.50±0.55,8.67±0.82,8.68±0.45,9.33±0.52,脂质体包裹组在同期均高于未免疫组和裸DNA免疫组,表明脂质体包裹质粒DNA免疫动物后,能增加动物对病毒感染的抵抗力和反应能力。

In 1980s, HFRS additionary occurred in spring season, the diseased cases and the dead cases accounted for 42.78%and 21.73%of that of the whole year respectively, the endemic area expanded to 14 areas where the mixed infection of Hantaan virus and Seoul virus were found. In 1990s, the diseased cases and dead cases were 43.68%and 37.55%of that of the whole year respectively, mixed infection of Hantaan and Seoul virus was found.

结果表明:山东省在60年代和70年代HFRS发病以秋冬季(10月~翌年1月)为主,其发病数占全年发病数的89.87%,病死数占全年的88.99%;疫区限于6个地区,疫区型别为姬鼠型。80年代,除秋冬季发病高峰外,出现春季(3~6月)发病高峰,其发病数和病死数分别占42.78%和21.73%;疫区扩大至全省14个地区,疫区型别为姬鼠型和家鼠型的混合型。90年代情况与80年代类同,春季发病与死亡数分别占全年的43.68%和37.55%;疫区型别仍为混合型。

It was known that ORF of SeMNPV egt was 1572bp long and encodes 523 amino acids through DNAstar software analysis. TATA boxes lies at 48~53 and 68~72bp of the 5'-nonencoding region upstream of a translational start site. Comparing with seven kinds of nuclear polyhedrosis virus and one kind of granulosis virus, it indicated that the SeMNPV egt gene is the highest match the Agrotis setegum nuclear polyhedrosis virus.It has 75% nucleotides and 79% amino acid identity.

采用DNAstar软件分析,与7种核多角体病毒及1种颗粒体病毒egt基因的同源性比较显示,SeMNPV egt基因与核多角体病毒egt序列同源性较高,其中与黄地老虎核型多角体病毒同源性最高,核苷酸和氨基酸序列同源性分别为75%和79%。

So we use all anti-virus biotics to suppress the virus activity, and then HBIG to bind down the residual virus works very well.

所以我们使用抗病毒药物来抑制病毒的活性,然后用乙肝免疫球蛋白来结合残留的病毒,这种办法非常有效。

Avian influenza virus was isolated from the caecal tonsil, faeces, and kidney of the domestic ducks. The isolated virus was identified as a highly pathogenic H5N1, with a haemagglutinin proteolytic cleavage site deduced amino acid sequence of ... QREKRKKR/GLFGAIAG ... In order to determine the pathogenicity of the isolate, eight 6-week-old specific pathogen free chickens were inoculated intravenously with the virus, and all birds died within 24 h after inoculation.

病毒抗原可以从鸭的盲肠,扁桃体,肾脏及粪便分离出来,并且确定这种病毒是高致病性的H5N1,还从血凝素蛋白分解位点推断出病毒的氨基酸序列,为了了解独立群的发病机理,对8只6周龄鸡静脉注射这种病毒,结果所有的均在注射后24小时死亡。

Another argument is that apes chimpanzee immunodeficiency virus contact time is not long, the virus carried by chimpanzees may be red top zanthopygia monkey, Cercopithecus monkeys or carried by other types of immunodeficiency virus variant from the new.

另一种说法是,黑猩猩接触猿类免疫缺陷病毒的时间不长,黑猩猩所携带的病毒可能由红顶白眉猴、长尾猴或其他种类猴子所携带的免疫缺陷病毒新近变异而来。

Apple latent vrius ; latent carrying percentage ; Chlorotic Leaf Spot Virus ; Stem Pitting Virus ; Stem Grooving Virus

苹果潜隐病毒;潜带率;褪绿叶斑病毒;茎痘病毒;茎沟槽病毒

The developed Indirect Sandwich ELISA was specific, the measure could not cross react to the negative antigen of duck swollen head septicemia virus, Duck Plague Virus, E. colibacillosis, Duck Hepatictis Virus.

建立的间接夹心ELISA法具有高度特异性,不与鸭肿头败血症阴性抗原、鸭大肠杆菌抗原、鸭瘟、鸭肝炎病毒抗原呈现交叉反应。

Three kinds of method of virus elimination-shoot tip culture, thermotherapy and treated with antiviral chemical-were compared and evaluated with detection of strawberry mottle virus and Strawberry mild yellow edge virus by multiplex RT-PCR in Fragaria ananassa cv. Hokowase infected with viruses.

以感染病毒的草莓品种宝交早生为试材,利用多重RT-PCR同时检测SMoV和SMYEV技术,对茎尖培养、热处理、抗病毒药剂处理3种脱毒方法进行了比较和评价,以期为草莓脱毒苗培育提供重要参考。

Four field strains of infectious bronchitis viruswere isolated from chicken kidney, trachea and lung of the young layer flocks in some regions of Shaanxi province. Biological characterizations of the virus were investigated by virus pathogenicity to chicken embryo and healthy chickens, hemagglutination test, hemagglutination inhibition test, dwarfing embryonated chicken eggs, electron microscopy and reverse transcription polymerase chain reaction. It demonstrated that allantoic fluid of each passage virus could hemagglutinate chicken red blood cells by treatment with 1% trypsin. The standard positive serum to IBV could inhibited the hemagglutination. The isolates could make respiratory and kidney diseases in chickens.

采集陕西省宝鸡市、扶风县、兴平市和榆林市等地鸡场疑似传染性支气管炎发病鸡病料,通过9~11 日龄非免疫鸡胚进行了病毒的实验室分离和传代,并对分离毒株进行了病毒的血凝性、血凝抑制性、致病性、鸡胚矮小化、电镜特征等生物学特性鉴定,结果表明,4 株分离株经1%胰酶处理后的各代病毒尿囊液均可凝集鸡红细胞,标准阳性血清可特异性的抑制其凝集性,回归试验可复制出与自然发病相同的病例,病毒传代物有明显的致鸡胚矮小化作用,透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子,将分离鉴定的4 株IBV 定名为BJ-04、FF-04、XP-03 和YL-04。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。