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It is reported, CIH1.2 virus is file virus, it is the first virus that can destroy computer hardware, virus can destroy the complete information on user system, below certain circumstance, can rescript systematic BIOS, bring about toxic computer advocate board be destroyed with hard disk, cause computer to cannot be started, the data on hard disk is missing.

据悉,CIH1.2病毒为文件型病毒,是历史上第一个可以破坏电脑硬件的病毒,病毒会破坏用户系统上的全部信息,在某些情况下,会重写系统的BIOS,导致中毒电脑的主板和硬盘被破坏,致使电脑无法启动,硬盘上的数据丢失。

Results By optimization test, capture antibody and detection antibody (from PVA detection kit, Agdia, USA) were finally diluted at 1:50 (4ug/mL) and 1:25 to be used in one step CBA, respectively. A total CBA reaction time of 2 h was needed. No cross reactivity with other plant virus similar to potato virus Y, lettuce mosaic virus and tomato ring spot virus were found. Detection sensitivity for positive control sample in one-step CBA was 4 folds higher than that in normal plate double antibodies sandwich enzyme-linked immunosorbent assay.

通过实验优化检测条件,最佳捕获抗体工作浓度为4μg/mL、最佳检测抗体工作浓度为1:25倍稀释、最佳反应时间为2h;与马铃薯Y病毒、莴苣花叶病毒、番茄环斑病毒等均未出现交叉反应;阳性样品经64倍稀释后依然可检出,检测灵敏度是传统微孔板ELISA的4倍。

By the research to computer virus characteristics,such as ruinousness, infectivity and uncertainty,according to the different classification of virus this paper analyzes the operating principle of computer virus respectively,and discusses some methods to clearing the computer virus.

因此,计算机病毒的防治对于维护系统的正常运行非常重要。1计算机病毒的特征计算机病毒是指编制或者在计算机程序中插入的破坏计算机功能或者毁坏数据,影响计算机使用,并能自我复制的一组计算机指令或者程序代码[1][2]。

It is indicated that MRV and street viruses have close phylogenic relation by analyzing the phylogenic tree of eachgene of MRV, and it is short evolutionary time between MRV and DRV by analyzingthe phylogenic tree of L gene. It is illuminated that MRV and street virus in China andAfrica have close phylogenic relation by analyzing phylogenic trees of other genes.Therefore, we can speculate that MRV is a Chinese street rabies virus isolated, whichis a new virus isolated because of variance and evolution of the rabies virus after itinfected mouse.

MRV的各个基因进化树表明,其与野毒株具有较近的亲缘关系,通过L基因的进化树分析,两个毒株的进化时间较短,其他基因进化树分析表明,MRV与亚洲和中国流行的野毒株具有较近的亲缘关系,所以推测MRV应该是中国固有的野毒株,由于某种原因感染野鼠后病毒进化变异而产生的毒株。

Abjective to express the Hepatitis A virus genome cDNA attenuated salmonella Typhimurium and to find the immunity to hepatitis A virus after administrated that salmonella Typhimurium with hepatitis A virus gemome to the mousehepatitis A virus coding region cDNA were inserted expressive plasmid PBV221 and then were transform into E coli DH5α.

使甲型肝炎病毒基因组cDNA能够在减毒伤寒杆菌中表达,从而通过口服此种减毒伤寒杆菌达到对甲肝免疫的作用。将甲肝病毒编码区cDNA插入高效表达质粒PBV221,转化大肠杆菌DH5α,筛选并鉴定阳性克隆。

In the rice leaves infected by RDV Yunnan isolate, the distribution of cells full of virus particles formed a belt which was a center of infection and multiplication of virus, around the cells fall of virus particles were some cells with the different extent of lesion as follows: disappearance of membrane system of chloroplasts, there were virus particles in some chloroplasts, and some sine or assembled virions in the mitochodria or cytoplasma but not in nuclei ,and intact subcellular structure in the cell far away from the infected center; In the rice leaves cell infected by Zhejiang isolate, some cells were full of virions, but the distribution of cells didn't form the belt, other cytopathological characters were similar to the cells infected by Yunnan isolate.

RDV云南分离株浸染的水稻叶片中,充满病毒粒体的细胞呈条带状分布,呈现出明显的侵染和增殖中心,在充满病毒粒体的细胞周围,相邻细胞有不同程度的病变,叶绿体膜系统消失,部分叶绿体中含有病毒颖粒,线粒体残体及细胞质中有分散或聚集的病毒粒体,细胞核中未见病毒粒体,离侵染中心较远的细胞中亚细胞结构较完整;RDV浙江分离株侵染的水稻叶片中,部分细胞充满病毒粒体,无明显的条带状分布,其他细胞病理特征与RDV云南分离株侵染的水稻叶片相似。

In order to improve the viral detection method in shrimp, a more rapid and convenient multiplex reverse transcription-polymerase chain reaction was established in the present study. The new method was used for detection of shrimp viruses, including Yellow-head virus, white spot syndrome virus, Taura syndrome virus, hepatopancreatic parvovirus, infectious hypodermal and hematopoietic necrosis virus and monodon baculovirus.

为了提高对虾病毒检测的快速性和简便性,建立了一种可同时检测6种对虾病毒的RT-PCR方法,并利用该方法对来自广西沿海地区的130份病虾样品中的黄头病毒、白斑综合征病毒、桃拉病毒、肝胰腺坏死病毒、传染性皮下组织坏死病毒以及杆状病毒进行了检测。

Choose medicaments correctly, reasonable use medicaments, in order to make sure with medicine safety is mixed effective will be henceforth the main task that clinical medicine learns, for solution control hospital the clinical current situation that use drug reachs existence issue, promote the development of the reasonable job that use drug, raise a hospital reasonable the level that uses drug, the prescription of two place has outpatient service of author general hospital, be in hospital analytic research, summary goes out unreasonable a few big states that use drug, undertake classified at the same time. 1 clinical unreasonable with medical analysis each division all applies 1 abuse of 1 · or antibiotic of blind application antibiotic extensively, utilization rate amounts to 70% above, among them couplet is used rate be as high as 40%, appear even 2 couplet, triplex above, the commonnest abuse is infection of the upper respiratory tract, be admitted to hospital give 2~3 to plant antibiotic cure. 1 · of 1 · 1 without point to this kind to the cure that ask for uses drug unreasonable with medicine main show is in the person that the virus sex appeal such as hepatitis of sex of common cold, chicken pox, berpes zoster, virus is caught, in infection of not amalgamative bacterium, point to without what apply antibiotic ask for next using medicine. Incontrollable virus affects antibiotic, increase hepatic burden instead to patient of virus sex hepatitis.

梗概: 正确选择药物,并合理使用药物,以保证用药平安和有效是今后临床药学的重要任务,为了解把握医院临床用药目前状况及存在新问题,推动合理用药工作的发展,提高医院合理用药的水平,笔者将医院门诊、住院两处的处方进行分析探究,总结出不合理用药的几大状况,同时进行分类。1临床不合理用药的分析1·1滥用或盲目应用抗生素抗生素各科均广泛应用,使用率达70%以上,其中联用率高达40%,甚至出现二联、三联以上,最常见滥用为上呼吸道感染,入院给予2~3种抗生素治疗。1·1·1无指征的治疗用药这类不合理用药主要表现在普通感冒、水痘、带状疱疹、病毒性肝炎等病毒性感染者,在未合并细菌感染,无应用抗生素的指征下用药。

Meanwhile many cases were diagnosed to be sero positive of anti dengue virus. Dengue I virus were also isolated from five cases. RT PCR test also indicated 4 out of 8 samples contained dengue I virus, which was the same type of dengue virus found in the epidemic areas in Guangzhou at the same time of outbreak.

进一步的调查在病例的血标本中分离出5株登革I型病毒,同时用RT—PCR的方法从8份标本中检出4份登革I型病毒阳性,与同期广州市登革热流行的病毒型别一致;取患者的恢复期血清作肥达氏反应,结果抗&O&、&H&的滴度与第一份血相比,均显著下降。

Results The concentration of Chlorogenic acid was 0.05mg/ml, 0.1 mg/ml,0.4mg/ml,0.8mg/ml respectively,it can respectively inhibit respiratory syncytia virus,Coxsackie B 3 virus,adeno associated 7 virus,adeno associated 3 virus and Coxsackie B 5 vi...

结果 氯原酸浓度分别为 0 。0 5mg/ml、0 。1mg/ml、0 。4mg/ml、0 。8mg/ml时,可分别体外抑制合胞病毒、柯萨奇B3、腺病毒 7型、腺病毒 3型和柯萨奇B5型。结论氯原酸对常见呼吸道病毒有较强的抑制作用

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呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

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