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The ribozymes 213 and 260 against the HCV 5-NCR can s ubstantially and specifically inhibit viral gene expression in HHCC.

我们构建的Rz213和Rz260真核表达载体能在HHCC细胞内表达，并有效地抑制HCV 5′NCR介导的翻译启动作用。

Peppermint oil helps to eliminate acne, dermatitis, eczema, itching skin and prevent viral infection.

它能消除痤疮，皮炎，湿疹和皮肤的搔痒更能有效的预防病毒的感染和蚊虫的叮咬。

For advertisers, or review requesters, the benefit is pretty obvious, considering the viral effects of the blog posts.

为广告客户，或审查请求者，好处是很明显，考虑到病毒的影响，该博客的职位。

We used the hepatitis C virus subgenomic replicon cell system to evaluate the effect of ethanol on HCV replication and viral protein synthesis.

我们用丙型肝炎病毒亚基因复制子模型，来评估酒精对丙型肝炎病毒的复制和病毒蛋白合成的影响。

In the study, to investigate the effects of mutations in dengue virus C gene and its encoded protein on viral replication, a series of dengue virus replicon mutants were constructed and their replication efficiencies were compared.

在登革病毒中，尚无C蛋白参与调节病毒复制的报道，而在C基因中是否存在其它影响病毒复制的RNA结构元件的问题也没有明确的答案。

Objective To construct expression vector of HBV infectious replicon and establish hepatitis B virus replication model in vitro, and observe the dynamic changes of viral replication and its sensitivity to antiviral durg.

目的 构建乙型肝炎病毒感染性复制子表达载体，建立HBV体外复制细胞模型，分析其复制特性及对抗病毒药物的敏感性。

Outcomes were classed into 3 groups, Sustained Viral Remission, Relapsers and Non Responders.

结局被分为3类：持续病毒学应答，复发和无应答。

The cell cultures were monitored by provirus DNA PCR, RT-PCR, reverse transcriptase activity assay and real-time RT-PCR, The results of RT activity and DNA PCR, RT-PCR were positive in the supernatant of cell cultures and viral particles were also clearly observed under electron microscope.

本试验基于代次毒分析结果，选取LTR 变异率较高的U3 区，以驴胎皮肤弱毒疫苗感染性分子克隆株pLGFD3-8 和驴强毒株感染性分子克隆株为父本操作系统进行基因替换，构建EIAV 非编码区强弱毒嵌合的全基因分子克隆，将阳性重组质粒命名为pLGFD9-12。

To understand the infectivity by porcine endogenous retrovirus with porcine skin fibroblast cell in vitro and in vivo, porcine skin fibroblast cell established by our laboratory were co-cultured with neo/HEK293 cell for the infection of RERV in vitro, and were subcutaneously transplantated to SCID (severe combined immuno-deficiency) mice for the infection of PERV in vivo, laying the foundation for valuation of biologic safety of xenotrans-plantation. The event of neo/HEK293 cells infected by PERV occurred during co-culture of porcine skin fibroblast cells with neo/HEK293 cells, expanding the rang of the infection of porcine endogenous retrovirus. Afterpig cells transplantated subcutaneously in SCID mice, the microchimerism (78.57%) of pig cells occurred widel, and there was phenomena of integration of PERV provirus (85.71%) in several organs or tissues remote from the injected sites, indicating infection of PERV in SCID mice in vivo. yet, there is no evidence of active viral replication in analysis of PERV env RNA of these tissues or organs.

为了解猪皮肤成纤维细胞PERV在体外和体内的感染性，通过建立猪皮肤成纤维细胞系，将所建细胞系与人胚胎肾293细胞体外共培养，并移植于严重联合免疫缺陷鼠皮下进行猪皮肤成纤维细胞PERV的体外和体内感染性实验，结果表明，猪皮肤成纤维细胞与人胚胎肾细胞共培养过程中，猪内源性逆转录病霉感染人胚胎肾细胞，进一步证实和拓宽了猪细胞PERV感染人细胞的范畴；猪皮肤成纤维细胞移植SCID鼠皮下后，导致SCID鼠发生猪细胞微嵌合(78.57%)和PERV在体内感染(85.71%)并且波及远离移植部位的多种组织或器官，但是并未检测出SCID鼠组织中表达PERV env RNA。

Accurate determination of HIV 1 proviral burden and viral load is very useful in prognosis of HIV 1 infected patients and in assessment of drug for therapy of AIDS patients.

准确测定HIV 1的前病毒载量和病毒载量的技术，在感染者预后和艾滋病患者药物治疗效果的评价以及艾滋病的其它研究方面，都具有十分重要的应用价值。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。