查询词典 viral

Bangte Lin I use a very useful, and I have asked dermatology Doctors say it is not a normal foul, if redness, smelly may be infected with the proposed investigation to blood sausage rules, see if it is a high lymphatic ah, if that is of a high viral infection

Bangte林我使用一个非常有用的，而我已要求皮肤科医生说，这不是一个正常的犯规，如果红肿，异味可能与拟议调查血肠规则感染，看它是否是一个高淋巴啊，如果这是一个高病毒感染

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The viral mRNA, which lacks the binding cap of a cell's normal mRNA, is then able to feed through the machine to create its own proteins.

病毒mRNA，缺乏细胞的正常mRNA 的约束盖帽，然后能哺养通过机器创造它自己的蛋白质。

Client bloodwork is monitored every 3 to 6 months for CD4+ T cell count and Viral Load.

每 3 到 6 个月检测一次患者血液中的 CD4+ T 细胞数和病毒载量。

FMD An acute, highly contagious degenerative viral disease of cattle and other cloven-hoofed animals, characterized by fever and the eruption of vesicles around the mouth and hoofs. It is usually not fatal.

Also called hoof-and-mouth disease 口蹄疫：家禽及其他偶蹄动物患的一种急性，传染性极强，由滤性细菌引起的衰退症，表现为高烧和口，蹄周围的生出小泡。

Higher Viral load was corelated with lower CD+4%and CD+4/CD+8 ratio.And the CD+4%in Symptomatic cases was around 14%.

病毒载量较高的感染者相应的CD+4淋巴细胞百分率较低，有临床症状的感染者其CD+4百分率多在14%左右。

The promotion of cotton growth factor, and enhance anti-viral immunity.

促进棉花生长因子，增强抗病毒免疫力。

BACKGROUND: Drug use is the most significant. risk factor for viral hepatitis B and C infection. Female drug users present different drug-using characteristics from the other sex. And what about the influence of these characteristics on hepatitis B and C virus double infection?

背景：吸毒行为是吸毒者感染乙型肝炎病毒(hepatitis B virus，HBV)和丙型肝炎病毒(hepatitis C virus，HCV)感染的主要原因，女性吸毒者吸毒行为有其自身特点，这些特点与HBV，HCV重叠感染有何关系？

Test results show that the recombinant adenovirus may be used in the viral treatment and gene treatment of tumor.

试验结果表明，此重组腺病毒可用于肿瘤的病毒治疗及基因治疗。

This suggests that a viral mechanism might be related to some forms of ALS.

这表明病毒机制可能与一些形态的ALS有关。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。