查询词典 viral

Methods The viral particles of recombinant adeno-associated viral vector 2-IRES-EGFP/MnSOD(rAAV2-IRES-EGFP/MnSOD) were injected into the perilymph through the round window membrane on the 6th week.To observe the feasibility of MnSOD gene therapy for protecting the cochlear function with the methods of Westernblot, ApopTag peroxidase apoptosis detection, MnSOD activity detection and effect on hearing.

方法在半乳糖注射的第6周(注射氨基糖甙类抗生素之前2周)将rAAV2-IRES- EGFP/MnSOD病毒液经圆窗膜注入拟老化大鼠的耳蜗外淋巴，通过免疫组化(ApopTag过氧化物酶凋亡检测)、Westernblot及MnSOD活性检测的方法观察MnSOD基因在对抗内耳氧化应激损伤中的干预性保护作用。

Because of the shortage of the ability on recognizing the genes and the scarceness of the tools for systemic research on the functions of hundreds and thousands of genes, mechanisms on anti-viral function of host cell after hepatitis B virus infection, and the interaction between anti-viral function and the replication of HBV still remain unclear.

由于对基因认识能力和系统的研究工具的缺乏，乙型肝炎病毒感染后，宿主细胞抗病毒效应的产生、作用机制以及与病毒复制表达的关系仍不清楚，甚至HBV在体内复制的机制也未能完全明了。

These are large, complex enveloped viruses with an outer lipid envelope studded with at least 10 viral glycoproteins, an intermediate tegument layer comprising at least 15 viral proteins, and an icosahedral nucleocapsid containing the double-stranded DNA genome.

这些是大，复杂被包围的病毒与一个外面油脂信封散布与至少10 病毒糖蛋白，中间tegument 层数包括至少15 病毒蛋白质，和一icosahedral nucleocapsid 包含double-stranded DNA 染色体。

Infected oligodendrocytes or type 2 PML cells with oval enlarged showing replacement of granular chromatin by basophilic or amphophilic, homogenized, ground-glass like viral inclusion bodies different from eosinophilic viral inclusion bodies of Cowdry type

感染的少突胶质细胞，或2型PML细胞，伴卵圆形拉长的核，显示颗粒状染色质变成嗜碱性或双嗜性、均质、毛玻璃样病毒包涵体（与Cowdry型的嗜酸性病毒包涵体不同）。

In eukaryotic cells,Toll-like receptors in endosomal membrane were show to recognize viral RNA in endosome and thereby initiate innate immunity pathways for virus resistance.By contrast,a cytosolic RNA receptor protein, RIG-Ⅰ(retinoic acid inducible gene-Ⅰ),has recently been identified for recognition of the viral RNA.

在真核生物体内，除了定位于内吞体膜上的TOLL样受体能够识别入侵机体的病毒RNA，启动机体抗病毒的固有免疫反应外，近年来发现了另一种能够识别病毒RNA的细胞质内受体——RIG-Ⅰ。

With its high clinical value, the product can be used to treat cancers, malignant ascites and pleural effusion, hydropericardium, leukemia and other viral infectious diseases (such as viral hepatitis, tuberculosis, herpes, AIDS), etc..

该产品可用于治疗癌症、癌性胸腹腔积液、心包积液、白血病及病毒性感染疾病（如病毒性肝炎、肺结核、疱疹、爱滋病）等。

Non-viral vectors have important safety advantages over viral approaches, including their reduced pathogenicity and capacity for insertional mutagenesis, as well as their low cost and ease of production.

对于非病毒载体的研究主要集中在靶向性、转染方式和转染效率等领域，较少关注病毒载体介导目的基因定点整合和目的基因长期表达等方面的研究。

Gene-activated matrix technology is a non-viral gene therapy strategy. Though with less impressive gene transfer efficiency than viral gene therapy, it has been used to deliver osteogenic factors locally and promote bone regeneration in long bones. The minimal safety concerns of GAM support its use in non-life-threatening conditions such as craniofacial osseous defects. However, the uses of GAM technology in intramembranous bone are largely unexplored.

活化基因基质技术是一种非病毒性的基因疗法，虽基因转移之效率一般不如病毒性基因疗法，但曾被用来局部产生骨生成因子而促进长骨缺损骨再生，其安全性高，应适於治疗颅颜骨缺损等不具生命危险性的状况，惟至今在膜内骨之相关研究仍甚少。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度；CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段；微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率，同时具有一定的靶向性，但总的转染范围仍然有限；采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预，发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低，CaN AβmRNA水平虽有下降趋势，但无统计学差异，提示质粒的用量及超声导入的参数有待进一步研究使其优化。

The results suggest that REV viral determination is same in both the group;REV viral antigen could be detected in the bursa of Fabricius, bone marrow, and glandularis venticuli, spleen, thymus, liver, lung, kidney and heart;viruses located maily in the immature lymphotaxis and reticular cell.

REV抗原阳性细胞的分布及形态特征基本相同，阳性细胞主要是正在发育成熟的淋巴细胞和上皮性网状细胞。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。