查询词典 violet-ear
- 与 violet-ear 相关的网络例句 [注:此内容来源于网络,仅供参考]
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It comes in many colors, including green, yellow, blue, violet, and a yellow-green variety, originally mined in Spain , commonly called "Asparagus Stone" because of its similarity in color to the vegetable.
磷灰石颜色繁多,有绿、黄、蓝、紫罗蓝等颜色。源产于西班牙的一种黄绿品种胜似蔬菜的颜色,人们通常称之为&黄绿磷灰石&。
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It is available as a handbag, backpack and shopping bag in the standard colours Black and Mocca as well as the Autumn colours Dust, Violet, Offwhite and Peanut.
它可作为一个手袋,背包,购物袋,在标准颜色黑色和咖啡站以及秋季颜色灰尘,紫罗兰, offwhite和花生。
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Violet rain is right on the bank the lunar cloud in water cries:"Do you also love what I love to want dead?"
紫雨在岸上对水中的月云喊:&你也爱我爱的要死吗?&
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Old World leafy-stemmed blue-flowered violet.
旧大陆多叶茎蓝花紫罗兰。
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The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.
(1)本研究用RT-PCR方法,从人组织细胞总RNA中扩增可溶性TWEAK胞外区(sTWEAK1和sTWEAK2)的cDNA序列及全长编码序列,用琼脂糖凝胶电泳分析PCR产物,胶回收目的基因片段,连接到pMD18-T克隆载体中,转化大肠杆菌K802,PCR和酶切筛选阳性克隆,全自动DNA测序验证序列;(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中,分别转化大肠杆菌BL21和TB1,PCR筛选和酶切鉴定,阳性克隆用IPTG诱导表达,表达产物用SDS-PAGE分析和Western blot验证融合蛋白;(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白;(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测,贴壁细胞用结晶紫染色法,悬浮细胞用磺酰罗丹明B染色法,酶标仪检测OD值;(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量;(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况;(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响;(8)用双荧光素酶报告基因检测法,测定表达产物对敏感细胞NF-κB的影响;(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上,用脂质体转染法转染HEK293细胞,PCR鉴定重组质粒。
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Small genus of delicate mossy bog plants having white or violet flowers.
沼泽地植物的一个小属,纤细苔藓状,花白色或紫罗兰色。
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Violet of eastern North America having softly pubescent leaves and stems and clear yellow flowers with brown-purple veins.
北美东部紫罗兰,有柔软的被绒毛的叶和茎,鲜黄色花,脉络棕紫色。
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Rose, osmanthus, violet, jasmine, lemon, rose, carnation, lily of the valley, Crape Myrtle and other fragrant flowers produced by volatile oils has significant bactericidal effect.
在热带干旱地区的部落,如多肉植物仙人掌的肉质茎气孔关闭白天,夜晚,打开,在吸收二氧化碳的同时,制造氧气,使负离子浓度室内空气增加。
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As to Chateau Lafite's attributes found in all vintages, it was an enlightened amateur that summed it up best by saying "...whatever the case, all the Chateau Lafite wines have an almond and violet aroma!"
至于拉菲的品质特征,无论是哪一个年份,都可引一位品酒行家的称赞作为评语,&凡入口之拉菲,皆拥有杏仁与紫罗兰的芳醇。&
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GOOD LUCK: Bayberry, cedar, cinnamon, honeysuckle, jasmine, lotus, mint, nutmeg, strawberry, vervain, violet.
好运:杨梅、雪松、肉桂、金银花、茉莉花、荷花、薄荷、豆蔻、草莓、马鞭草、紫罗兰。
- 相关中文对照歌词
- Violet
- Violet
- Under A Violet Moon
- Violet
- Violet Eyes
- The Violet Fire
- Sumiregusa (Wild Violet)
- Violet
- Violet Skies
- Violet Stars Happy Hunting!
- 推荐网络例句
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But we don't care about Battlegrounds.
但我们并不在乎沙场中的显露。
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Ah! don't mention it, the butcher's shop is a horror.
啊!不用提了。提到肉,真是糟透了。
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Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.
Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。