查询词典 violet blue

For example: orange, violet and green in art; red, blue and green in printing.

美术方面的橙，紫和绿色；印刷方面的红，蓝和绿。

It comes in many colors, including green, yellow, blue, violet, and a yellow-green variety, originally mined in Spain , commonly called "Asparagus Stone" because of its similarity in color to the vegetable.

磷灰石颜色繁多，有绿、黄、蓝、紫罗蓝等颜色。源产于西班牙的一种黄绿品种胜似蔬菜的颜色，人们通常称之为&黄绿磷灰石&。

Old World leafy-stemmed blue-flowered violet.

旧大陆多叶茎蓝花紫罗兰。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Perennial of damp places in mountains of Eurasia and North America having dull-colored blue or violet flowers.

欧亚和北美山区潮湿地区的多年生植物，暗蓝色或紫色花。

Other color variations for budgies are yellow, blue, violet, albino and unique color mixes made by breeding different colored budgies with each other.

其他颜色的变化budgies是黄，蓝，紫，白化及独特的色彩的组合，所作出的育种不同颜色budgies与对方。

Ultramarines: These colourants are seen in cosmetic uses in vibrant blue, violet, and pink shades.

ultramarines ：这种着色剂在化妆品中用作蓝，紫，粉红和。

As you watch your flame/spirit, Let the violet flame enlarge and envelop the blue, green, yellow, orange and red of the fire.

当你注视你的火焰/灵魂，让紫罗兰火焰放大和包围蓝色，绿色，黄色，橙色和火的红色。

Color is used sparingly,with a core palette of grays and blacks brought to life wieh touches of vibrant yellow, royal blue and violet, perhaps used as no more than a flash color for the cuffs or collars of a shirt.

颜色是用来节制，一个核心的调色盘格雷斯和黑人所带来的生命wieh触及充满活力的黄色，皇家蓝色和紫色，或许用作不超过1闪光彩色为袖口或衣领的衬衫。

You can for instance select blue, lilac, violet, pink and salmon colored Freesias.

比如你可以选择蓝色、丁香、紫、粉红鲑、有色

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。