查询词典 vicilin

SDS-PAGE technique separated the globulin polypeptides into three distinct groups with molecular weights about 50000, 30000-40000 and 15000-20000. Broad bean legumin and vicilin were prepared and their purity were measured by SDS-PAGE.

为进一步了解蚕豆球蛋白的结构和功能性质，对球蛋白中的两种主要组分—7S和11S球蛋白进行了分离纯化，并采用SDS-PAGE鉴定了两种组分的纯度。

The molecular weight of broad bean vicilin and legumin measured by gel filtration chromatography were 135000 and 351000, DSC showed that their denature temperatures were 72℃ and 87℃ respectively. The legumin had a lowest solubility at pH 4. 5-5. 0 whereas the vicilin at pH5. 5. Vicilin and legumin were all possessed low levels of α-helical and high β-sheet secondary structure fractions. The total content of α-helical,β-sheet and β-turn fraction of vicilin and legumin reached to 90% and 78% separately showed that they had a high ordered structure in solution.

对两种组分的凝胶层析表明7S和11S的相对分子质量分别为135000和351000；DSC分析显示其热变性温度分别为72℃和87℃；溶解度分析表明：11S球蛋白在pH4.5-5.0之间，7S球蛋白在pH5.5时具有最低的溶解度；圆二色性分析结果显示7S和11S球蛋白的β-折叠结构含量分别高达40.5％和38.1％，证明二者都属于β-折叠型蛋白质，二者的α-螺旋、β-折叠和β-转角结构的含量总和分别达到了90％和78％，从而说明它们在溶液中的结构是高度有序的。

The α-helical structure of the globulin is an important factor to forming and stabilizing the protein emulsion. The particle size distribution of legumin and vicilin hydrolysates were measured by dynamic light scattering, the particle size of legumin hydrolysates was decreased at first then increased during the papain hydrolysis, but the vicilin hydrolysates decreased continuously, it demonstrated that the legumin hydrolysates might have a re-aggregation in the solution which the vicilin hydrolysates did not have.

采用动态激光光散射分析了水解产物的粒径分布，结果表明：随着水解的进行，11S球蛋白水解物在溶液中的粒径出现先减小后增加的趋势，而7S球蛋白水解物的粒径呈递减趋势，说明11S水解产物分子间发生了新的选择性定向和聚合，而7S水解物分子间未发生二次聚合。

Using papain to hydrolyze the legumin and vicilin respectively showed that it could obtain higher DH from legumin than from vicilin, and the emulsifying activity of legumin hydrolysates was also increased more than vicilin hydrolysates. It was easier to hydrolyze the β-sheet fractions than α-helical fractions of broad bean legumin by papain, but to vicilin the β-sheet fractions was difficult to be hydrolyzed.

发现Papain对11S的水解程度大于7S，11S水解物的乳化活性也高于7S水解物；对水解物的圆二色性分析表明：蚕豆11S球蛋白的β-折叠结构易于被木瓜蛋白酶作用，而7S球蛋白的α-螺旋结构易于被木瓜蛋白酶作用；蛋白质分子中的α-螺旋结构对乳状液的形成和稳定起着重要作用。

Its cDNA had been cloned by RT-PCR. The results of comparison on sequence homology suggested that Luffin P1 may be derived from a vicilin like precursor which is a storage protein in the seeds of Luffa cylindrica.

本文利用3'RACE法克隆了它的cDNA，并构建了它的融合表达质粒pGEX-4T-S2，该质粒在大肠杆菌中获得了成功表达。

In this research, the complete ipt gene was cloned from Agrobacterium pTiAch 58 Hind Ⅲ fragment and its promoter was re-constructed so that following three chimaeric genes were created:(1) ipt promoter plus ipt coding region and terminator ,(2) RuBP SSU 301 promoter from petunia plus ipt coding region and terminator ,(3) pea seed-specific vicilin promoter plus ipt coding region and terminator .

将ipt基因克隆后对其启动子进行了改造，分别构建了如下三种基因：（1）ipt启动子+ipt编码区和3′区，（2）磷酸核酮糖羧化酶小亚基启动子SSU 301+ipt编码区和3′区，（3）豌豆种子特异性启动子vicilin+ipt编码区和3′区。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。