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Methods: Virus proliferation assay, cell viability assay and Western blot were performed to assess the selective replication and cytolysis of CNHK500 in telomerase positive liver cancer cells Hep3B, HepGⅡ, SMMC7721 and in normal cells.

方法利用病毒增殖实验、细胞活力实验、蛋白印迹分析来检测增殖病毒CNHK500在端粒酶阳性的肝癌细胞株HepGⅡ、Hep3B、SMMC7721及正常细胞中选择性增殖和溶解细胞的特性。

Methods: The telomerase activity in breast cancer cells and MRC-5 cell line were detected semi-quantitively by TRAP-ELISA assay. Virus proliferation as- say and cell viability assay were used to evaluate the proliferation and cytolysis selectivity of CNHK300, and the results were compared with those of wtAd5. The breast cancer cells were treated with CNHK300 combining paclitaxel to evaluate the killing effect of the combined regimen.

用TRAP-ELISA方法检测乳腺癌细胞株(MDA-MB-453、SK-BR-3)和正常成纤维细胞株MRC-5的端粒酶活性；进行病毒增殖实验和细胞生长抑制实验，并与野生型腺病毒wtAd5进行对比，验证CNHK300选择性复制和选择性杀伤能力；用细胞生长抑制实验进一步考察CNHK300与化疗药物紫杉醇联合应用对乳腺癌细胞的杀伤作用；用Western blot检测腺病毒E1A在细胞中的表达。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Cultured ECV304 were either not treated, treatedwith 10 M of hydrogen peroxide (H2O2), treated with TNF-(40 ng/mL) alone, TNF- in the presence of 10 M of H2O2, or propofol plus H2O2 for 24 h. Cell viability was measuredby lactate dehydrogenate assay.

本研究取人血管内皮细胞 ECV304经培养后分为以下几组：对照组、过氧化氢预处理组、 TNF-预处理组， TNF-复合 H2O2预处理组、丙泊酚复合 H2O2预处理组。

Exogenous E2 alone has no effect on cell viability and apoptosis of H9C2 cells, but it can decrease the sensitivity of H9C2 cells to H_2O_2,hence,protect cells against apoptosis caused by oxidative stress.2Exogenous E_2 has downregulation effect on TβRⅠand TβRⅡof H9c2 cells,blocking ER may delete this effect of estradiol.H9c2 cells express ERβonly,suggests that E_2 may exert modulating effect on TGF-βpathway through ERβ.

外源性E2本身并不能影响H9c2细胞凋亡，但能够降低H9c2细胞对H_2O_2的敏感性，保护细胞抵抗氧化应激引起的凋亡。2外源性E2对H9c2细胞的TβRⅠ以及TβRⅡ具有下调作用，阻断ER可消除E2对TβRⅠ和TβRⅡ的下调作用；E2可进一步通过TGF-β通路降低受体后磷酸化smad2/3的水平；H9c2细胞仅表达ERβ，提示E2可能是通过ERβ发挥其对TGF-β通路的调控作用。

Duringnatural desiccation, change of temperature(10℃～25℃)have no influenceon cells viability.

阴干处理期间，温度的变化(10℃～25℃)对冷冻后的紫菜细胞活力没有明显影响。

Compared with diploidy , the seedlings of Pasthyrostachys juncea with tetraploidy have stronger viability , higher nutrient contents and better tolerance of lower temperature , drought and other extremely environments .

与二倍体新麦草相比，染色体加倍后的四倍体新麦草的种苗生活力强，种子营养成分含量高，对低温，干旱等极端环境的适应能力强。

His reasoning was disarmingly intuitive: in our hominid predecessors, communities of more sympathetic individuals were more successful in raising healthier offspring to the age of viability and reproduction -- the sine qua non of evolution.

他的理由完全是直觉性的：在我们原始祖先那里，那些其成员更具有同情心的群落在养育更加健壮的达到生育与繁殖能力的后代方面更成功——这是进化的必要条件。

Eliminates the female to get employed the discrimination, needs the long time insistence with diligently, certainly not strength, the temporary merit the, needs the entire social the joint effort, needs from the system to the idea different level consummation and the transformation; Needs the public opinion to finish for the feminine full employment propagandizes positively; Needs to guide the family and society's attention positively pays attention to the feminine reality each kind of need; Needs feminine oneself to improve own cultural quality and each skill diligently, prepares for the full employment, the transformation idea, forms the independent viability.

急！！！中译英，英语文章，修改语法错误消除女性就业歧视，需要长时期的坚持和努力，绝非一己之力、一时之功，需要全社会的共同努力，需要从制度到观念不同层次的完善和转变；需要社会舆论为女性充分就业作好积极地宣传；需要积极引导家庭和社会的注意力关注女性现实的各种需要；更需要女性自身努力提高自己的文化素质和各种技能，为自己充分就业做好准备，转变观念，形成独立的生活能力。

Methods Eukaryotic expression plasmid pCMVCD was constructed , and identified by re2 striction endoenzyme digestion. CD gene was transfected into NSCs from new2born Wistar rats using Lipofec2tamine2000. Positive clones (named NSCs/ CD cells) were screened by G418 presence. 52Fluorocytosine (52FC) ad2ministration of different concentrations were incubated with NSCs/ CD cells. NSCs/ CD cells viability ratios were mea2 sured by MTT assay.

通过构建真核表达质粒pCMVCD ，限制性内切酶消化鉴定后，采用Lipofectamine 2000 脂质体介导法转染新生大鼠室管膜下区神经干细胞(Neural stemcells ， NSCs)，G418 筛选阳性克隆，加入不同浓度的52氟胞嘧啶(52Flourocytosine ， 52FC)，MTT比色法测定NSCs的生存率。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。