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In this study, in utero transfection plasmid DNA method was set up to embryonic mouse. The GFP expression vector pCAGGS-IRES-EGFP was microinjected into the lateral cerebral ventricle of E 14.5d mouse embryos through the uterine wall. Then, the 30 V square-wave pulse was delivered across the head for 5 times and interval 50m sec by electrodes in parallel with the median raphes.

本研究旨在通过子宫内电穿孔法，建立鼠胚大脑皮层体内基因转移体系：将带有绿色荧光蛋白的表达载体pCAGGS-IRES-EGFP显微注入胚胎期14.5 d的鼠胚侧脑室中，电极沿与脑中缝平行的方向夹持鼠脑，在电压30 V，脉冲时间50 ms，脉冲数5的条件下方波电击，将外源基因转移到室管膜层细胞。

However, when AV interval was shorter than 80 msec and longer than 180msec, there was reduction of LVOTG, yet the CO was also reduced. The results showed that the hemodynamic improvement must depended on the state of pre-excitation of ventricle as well as the optimal left ventricular filling.

且在AV间期为100至120毫秒时出现最佳血液动力学效应，而AV间期在小于80毫秒、大于180毫秒时，虽有左室流出道压力阶差的降低，但心输出量均有下降，提示血液动力学的改善必须依赖右心室的提前激动状态及最佳的左心室充盈。

Results :We report a case of bronchogenic cyst occurring in the left ventricle of a 2-year-old boy presenting with intermittent precordial pain, although the true diagnosis was missing preoperatively, complete excision of the cyst was achieved via a left anterolateral thoracotomy.

结果：1例罕见的左心室壁先天性支气管囊肿，术前未获明确诊断，但经左前外侧切口进行了完整切除，病理检查提示囊肿内壁通常由假复层柱状纤毛上皮覆盖，周围含有软骨和支气管黏膜腺体。

CGRP content in myocardium gradually enhanced from normal group to controls group, and model group's was the highest. We found immunopositive neurons and nerve fibers of nNOS and CGRP by immunochemistry in normal myocardium. They were mainly round-shaped and oval-shaped, also tadpole-shaped、 shuttle-shaped、triangle-shaped、ring-shaped and irregular-shaped. Nv of neurons and nerve fibers in atria were more than in ventricle in some degree; Those in right part of heart were more than in left. Morphology of CGRP neurons changed in model group, the number of ring-shaped neurons increasing, while the round-shaped and oval-shaped decreasing, and Nv and Sv of model group were markedly less than normal. Expression of nNOS mRNA was overly higher than normal group, but CGRP was opposite.

心肌组织CGRP的含量由正常组、实验对照组至CHF组逐渐升高，差异显著；免疫组化研究表明心肌组织中存在nNOS和CGRP免疫阳性神经细胞及纤维，细胞形态多样，正常多以圆形和椭圆形为主，还有梭形、蝌蚪形、三角形、指环形及不规则形，细胞密度心房高于心室，右心高于左心；CHF组CGRP神经细胞形态有一定改变，淡染的指环形细胞增多，而深染圆形或椭圆形细胞减少，其面、数密度均明显低于正常组，差异显著；用RT-PCR测得CHF组nNOS mRNA极度表达，显著高于正常组，而CGRP表达明显低于正常组。

Methods: Twenty-four male SD rats were randomly divided into normal control (NC, n=6), protein C activator (PCA, n=6), lipopolysaccharide (LPS, n=6) and LPS+PCA group (n=6). NC group were given normal saline and PCA group were injected protein C activator (0.4 mg/kg) through vein of the tail. LPS group were administered lipopolysaccharide (10 mg/kg, 4h) by intraperitoneal injection method to make the model of septic shock rats. After the animal model was made, LPS+PCA group were injected protein C activator (0.4 mg/kg) through vein of the tail. 30 minutes after the injection, each rat was tested by Medlab biosignal analyzing system for observing mean arterial pressure and left ventricle function, the activity of LDH and iNOS in myocardium and the cuttings of myocardial tissues.

取SD雄性大鼠24只随机分成对照组、PCA组、LPS组和LPS+PCA组四组，对照组尾静脉注射生理盐水，PCA组尾静脉注射蝮蛇毒蛋白C激活物，LPS组腹腔注射LPS (10 mg/kg， 4h)的方法建立败血症休克的实验动物模型，在建立败血症的实验动物模型的基础上，尾静脉注射PCA (0.4 mg/kg)为LPS+PCA组，每只大鼠均于药后30 min时运用Medlab生物信号采集处理系统记录平均动脉压和左心室功能指标，并测定心肌中乳酸脱氢酶活性、诱导性一氧化氮合酶活性及心肌病理切片。

Methods Neonate rats were irradiated with a single injection of 2 μl enriched*" 235 uranium( 235 U)into the lateral ventricle of the rat brain on the first day of life(less than 24 hours after birth)at doses of 0,1,5 and 10 μg of*" 235 U,respectively.

新生大鼠侧脑室注射 2 μl浓缩铀溶液后，多方位地观察不同浓度的2 35U对早期生长发育的影响，放射自显影示踪术观察脑内放射性核素行径定位。

Objective To evaluate the value of acoustic densitometry in detecting myocardium damage of the left ventricle secondry to hypoxic pulmonary hypertension.

目的探讨声学密度定量技术定量评价缺氧肺动脉高压后左室心肌损害的应用价值。

OBJECTIVE: To evaluate the value of acoustic densitometry in detecting myocardium damage of the left ventricle secondry to hypoxic pulmonary hypertension.

目的：探讨声学密度定量技术定量评价缺氧肺动脉高压后左室心肌损害的应用价值。

It not only provides the direct left ventricle unloading effect, but also augments the aortic pressure which in turn increases the coronary flow. For th ese reasons, the PUCA pump may have a prosperous future.

搏动性导管泵的作用介于 IABP和高辅助量的心室辅助装置之间，能起到既减轻前负荷，又提高主动脉压并增加冠状动脉灌注的作用。

Methods After heart failure model was induced successfully in 10 sheep, PUCA pump was inserted through aorta and the aortic valve orifice with its tip located in the left ventricle. It was then activated to support the hemodynamics for 3 hours. Hemodynamic parameters were monitored and recorded before thoracotomy, heart failure, and every 60 min after the support. Platelet and plasma free hemoglobin were tested during the experiment.

方法对10只绵羊诱导左心衰竭模型建立成功后行PUCA泵辅助3h，监测并记录血流动力学参数，采动脉血人工计数血小板并测定血浆游离血红蛋白；经辅助3h后检查肾、隔膜泵及导管血栓形成的情况。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。