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Parkinson s disease is a degenerative disease of the basal ganglia causing tremor at rest, muscle rigidity and hypokinesia Gene therapy is most likely to cure PD completely, and the most promising one among all the treating ways But its effectiveness and safety have a strong relationship with the vectors chosed This article introduces some usually used virus vectors and their characteristics as well as the trend of virus vector in PD s gene therapy

帕金森病是一种神经节退行性疾病，主要以静止震颤，肌肉僵直和运动减少为临床症状。在其各种治疗方法中基因治疗最有望彻底治愈PD，最有发展前景。但其有效性与安全性与其所选用的载体有很大关系。本文介绍了基因治疗中常用的病毒载体以及它们的特性，并指出PD基因治疗中病毒载体的发展趋势。

The signature vectors are estimated by projection onto the intersectant subspaces. Based on the estimated signature vectors, the multiuser detector is constructed.

利用到相交子空间的迭代投影估计得到包含信道信息的特征矢量，以此构造多用户接收机。

The protozoa were isolated from field animal blood, vectors, pathogenic tissue and secretions. Based on differences of the pathogen morphology, vector species, experimental infected animals, single parasite clone and parasitic tissues, protozoiasis pathogens from different areas and animals in China were isolated and the vectors and transmission mode were also determined by vector transmission trial. Sequence analysis of 18S rRNA gene was used to study the phylogeny of partial isolated protozoa.

自野外采集动物血液、传播媒介、相关组织、排泄物等，根据对病原形态鉴定、传播媒介鉴定、实验性感染易感动物、单一虫株克隆、不同致病部位等方法，对我国不同地区、不同家畜的原虫病病原进行分离，并通过媒介传播试验确定传播媒介的种类与传播方式；采用18S rRNA基因测序方法，对本实验室已分离的部分原虫进行进化关系研究。

In this algorithm,by constructing a UserlD-URL revelant matrix similar customer groups are discovered by measuring similarity between column vectors and relevant web pages are obtained by measuring similarity between row vectors;frequent access paths can also be discovered by further processing of the latter.

第四是本文将传统数据挖掘过程中的各种关键技术，引入到对Web使用信息的挖掘活动中，结合关系数据库的特点设计并实现了一个具有可广西人学颀士学位论义视化功能的Web使用挖掘系统WLGMS。

In the third part, the simulation research on asynchronous motor DTC system base on six voltage space vectors and twelve voltage space vectors are realized in MATLAB/Simulink. Through comparisons of the two simulation results, the method of minifying torque ripple is obtained.

第三部分，运用MATLAB/SIMULINK进行了基于六矢量和十二矢量的两种异步电动机直接转矩控制系统的仿真研究，搭建了系统的仿真模型，并对仿真结果进行了对比，得出减小直接转矩控制转矩脉动的方法。

Combining the advantages of fixed search pattern and spatial correlation search pattern,the algorithm employs the high search accuracy of DS and the high search speed of ARPS.It performs zero motion prejudging for immobile macroblocks between the two adjacent frames to reduce the computational complexity,and employs the spatial correlation of motion vectors to improve the quality of predicted motion vectors.

该算法利用DS算法搜索精度高和ARPS算法搜索速度快的特点，综合固定模式搜索和空间相关搜索两方面的优点，对于相邻两帧图像中的不变宏块采用零运动预先判断以减少算法的计算量，并利用运动矢量的空间一致性提高预测运动矢量的质量。

This method reshapes the SAR images matrix into vectors, and analyzes the matrix which is composed of the two vectors with PCA process, getting a conclusion that the minor component image is the change portion.

该算法将SAR图像转化为列向量，对两个图像向量组成的矩阵进行主分量分解，其图像的变化部分则表征为矩阵的次分量成分。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

During the procedure for searching k nearest neighbors of tested sample, the particle swarm moves jumpily, and those document vectors that are impossible to be the k closest vectors are kicked out quickly.

在搜索k近邻的过程中，粒子群跳跃式移动，掠过大量不可能成为k近邻的文档向量，从而可以快速找到测试样本的k个近邻。

Firstly, through applying Hilbert-Sobolev norm to measure fidelity term, a total variation filter is used to smooth the normal vectors of the level curves of a noise image. And then, a model is constructed to find a surface which fit smoothed normal vectors.

该方法首先引入负指数Hilbert- Sobolev范数度量逼近项，对图像水平曲线的法向量场进行全变差正则化磨光，然后构造出一个曲面拟合模型，拟合磨光后的流场。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。