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The main resuLts of this study are concluded as follows:(1) DAS and DAK genes were amplified from the genomic DNA of C.boidinii and P. pastoris , respectively. The plant expression vectors, pK2-Prbcs-DAS and pH2-Prbcs-DAK were constructed for DAS and DAK genes by gateway technology.

为了获得具有自主知识产权的基因,用于进行植物甲醛代谢途径的遗传工程操作,本研究也尝试利用国内的资源和条件,分离能代谢一碳化合物的酵母菌株,为将来开展甲基营养型酵母菌的分子生物学研究,克隆C1代谢途径相关基因奠定研究基础。

In these two plant expression vectors, the expression of the DAS and DAK genes was under the control of tomato Rubisco-3c promoter. The Prbcs promoter contains a transit peptide sequence which allows the location of the expressed DAS and DAK into chloroplasts. The DAS and DAK genes were introduced into Nicotiana tabacum and Petunia hybrida by Agrobacterium tumefaciens- mediated procedure.

研究结果如下:(1)根据已克隆的C.boidinii酵母菌DAS和P.pastoris DAK基因的序列设计特异性引物,通过PCR分别从这两种酵母菌的基因组中扩增DAS、DAK基因,利用gateway技术构建DAS、DAK基因的植物表达载体pK2-Rbes-DAS、pn2-Rbcs-DAK,在构建好的植物表达载体中,DAS和DAK基因的表达都在番茄Rubisco小亚基rbcS-3C启动子的控制之下。

Based on the return map and the principle of closed vectors, a new method is proposed to extract unstable periodic orbits embedded in chaotic attractors. As examples, the UPOs embedded in chaotic attractors of Logistic, Hénon and Lorenz are extracted respectively by this method. And our results of Skewed Hénon map also be compared with Nusse's. These results suggest that this method is valid for unstable periodic orbits from period one to period infinite of arbitrary dimension chaotic system. The dynamic considerations of spiking and UPO coding for individual neuron and neural system under external periodic and chaotic exciting stimulus also be studied in this dissertation. A lot of spiking phenomena, such as synchronization, period, and chaos appear alternatively with the changing of the stimulus frequency. For the small stimulus frequency the neuron could completely convey the periodic signal in synchronous anti-phase into interspike intervals sequences. For the slow time–scale chaotic input, the output two ISI sequences are reciprocally related to input signals, and their oscillation wave shape in time course can be derived from that of the input signals variation, furthermore, the similar input sequence and order of UPOs, distribution of LES and value of KYD remain in attractors reconstructed from ISI sequences.

发现周期信号在单个神经元传递过程中,随着激励频率的改变,神经元输出的峰峰间期interspike interval时间序列呈现出周期、混沌和准周期的交错变化,特别当外加激励信号频率较低时,周期信号可以通过神经元ISI序列以反相同步的周期运动形式传递下去;同时无论是周期还是混沌激励信号,在神经系统中的传递均与其自身强度和神经元之间的耦合强度的大小密切相关;快变时间尺度的混沌激励信号在耦合的神经系统传递过程中,会造成大量基本信息的丢失;而慢变时间尺度的混沌激励信号在神经系统传递中,它的非线性特征信息,如混沌吸引子、不稳定周期轨道、Lyapunov指数谱和分形维数,会通过系统输出的ISI序列部分地重现出来,如与输入慢变时间尺度的混沌激励信号相比,神经系统输出的ISI序列具有:相似几何形状的混沌吸引子、相近的Lyapunov指数谱和分形维数、局部结构相同的不稳定周期轨道的排列方式。

"Transform",The Laplace transform can be used to solve the state equations for the state and output vectors.

&,&拉普拉氏转换可以用来解状态方程的状态向量和输出向量。

Then we use modified nodal analysis to transform the problem into an Ax=b linear algebraic problem where A is a n*n matrix, x and b are n*1 vectors.

然后,利用修饰节点分析方法,我们可以把原始问题转换成一个Ax=b的线性代数问题。在此,A是一个的矩阵,x和b是的向量。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。