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From the frequently used way that applying probe vectors for choosing promoter to the using of PCR, there were lots of ways for promoter cloning. Afterwards, a series of techniques based on PCR for promoter cloning such as I-PCR, P-PCR, SSP-PCR, YADE, TAIL-PCR were developed in succession. They offered more reliable and reasonable ways of cloning the promoter.

着重介绍了启动子克隆的方法,从常用的利用启动子探针型载体筛选启动子到PCR方法的应用,及此后相继出现的一些基于PCR法的克隆启动子技术,像I-PCR, P-PCR, SSP-PCR, YADE, TAIL-PCR等,为克隆启动子提供了更可靠,更合理的方法。

It was conclude that wind and weevils were effective pollination vectors for C. elongata, which inconsistent that Zamiaceae was host-specific insect pollination.

可见,风媒和虫媒都是越南篦齿苏铁的有效传粉途径,与泽米铁类植物为专一寄主性昆虫传粉的结论不一致。

A bifurcating tree discriminant classification algorithm is developed. On the basis of training the images of a same class with some typical distortions, the distribution of the feature vectors of various classes in the sample space is optimized by using the discriminant analysis.

利用体全息存储系统的海量存储和内容寻址功能,高并行度地实现了基于本征图象的光学特征提取过程,充分显示了光计算及光存储技术在图象识别中的潜力。

Bisected the image into four quadrants according to the typhoon center, the number of motion vectors satisfied the correct motion conditions should reach the maximum.

在基于台风中心划分的四个象限内,符合正确的运动条件的云系微元所占的比例应最大。

The way of promoter cloning is important for constricting gene engineering vectors and expressing the aim proteins.

启动子的克隆对于构建基因工程载体,表达目的蛋白有着重要的意义。

Neglecting noise, to facilitate this, time lagged version matrix of convolved signal and seismic wavelet banded convolving mixture matrix are used ,by multiplying the reflectivity vectors, to construct a basic ICA model.

基于对地震反褶积本质上是一个盲过程的认识,引入高阶统计学盲源分离技术——独立分量分析实现地震盲反褶积。

Meanwhile, a concise method based on resource vectors is presented for determining whether a system has possible deadlocks.

基于资源向量的概念,提出了一个简单方法,用于判断系统是否具有可能的死锁。

Based on decompounding of row vectors and line information, a fast evaluation model of jamming effect is built, and the allocation tactics for radar jamming resources, jamming effect after allocating are studied by using the principle of Euclid close degree.

在分解隶属度矩阵行向量信息与列向量信息的基础上,构建了一种快速评估雷达干扰效果的模型。利用Euclid贴近度原理对雷达干扰资源的分配策略、分配后的干扰效果进行了研究。

Experimental results show the correct vectors of camera are reserved while dithers are eliminated, the peak signal-to-noise ratio of smoothed video is higher than that of the original video.

实验表明该算法较好地去除了视频序列的高频抖动,同时保留了摄像机的主动运动,稳像后视频序列的峰值信噪比明显提高。

METHODS The HCV core gene coding region was inserted into the eukaryotic expressive plasmid pcDNA3, then the recombinant plasmid pcDNAHCV-C was constructed and expressed transiently with LipofectAMINE in the SP2/0 cells. After purification, these plasmid directly or encapsuled wit h Lipofect-AMINE were injected into BALB/c mice. HCV core antibody from immunized mice could be detected by ELISA. RESULTS The enzyme -cutting identification showed that HCV core gene fragment had been cloned into pcDNA3 eukaryote vectors.

人工构建包含HCV C基因片段的真核表达载体pcDNAHCV-C,在证实其可以在真核细胞中表达之后,将其用脂质转染剂包裹,形成LipofectAMINE-pcDNAHCV-C脂质混合物,将该混合物或单纯pcDNAHCV-C直接注射BALB/c小鼠股四头肌,以空载体pcDNA3做对照,ELISA法检测血清中抗体产生水平。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。