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Lactobacilli as live vaccine delivery vectors: progress and prospects .

猪传染性胃肠炎和猪流行性腹泻疫苗研究进展。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下:1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草,获得三个单基因和一个双基因的RNAi沉默表达植株,快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加;叶组织内Na+离子含量和K+离子含量均略高,但差异不显著;K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降,但差异不显著;K+离子含量比对照显著降低。

This magnetic arrangement along with the resulting radial currents from the Neodymium layer creates the roller's magnetomotive forces into motion at right angles to both the electric and magnetic vectors.

这磁性的安排与由此产生的径向水流从钕层创造了滚子的 magnetomotive部队运动成直角的电场,磁场载体。

The SEG's stators and rollers are magnetic components uniquely magnetized or imprinted with magnetic patterns and matched like a planetary gear. This magnetic arrangement along with the resulting radial currents from the Neodymium layer creates the roller's magnetomotive forces into motion at right angles to both the electric and magnetic vectors.

SEG的定子和转子是具有相反磁性的磁力部件,互相吸引起来就像一种行星齿轮,这种磁力装置通过金属钕涂层产生径向电流,这种电流产生的电场力和磁场力方向互相垂直,从而推动转子移动。

Micrococcal lysis assay of the milk samples showed that the cDNA was efficiently expressed in the mammary glands with similar expression levels ranging from 60 to 87 mg/L. Different tissues of each vector-injected mouse were assayed for transcription of hLYZ mRNA by dot-blotting and for expression of hLYZ activity by Micrococcal lysis assay. The results showed that expression of the three recombinant vectors was mainly restricted to mammary glands with some degrees of ectopic expression in spleen, intestines and/or kidney.

注射后72 h采集乳样,经微球菌溶解实验证明,3组小鼠乳汁中hLYZ的表达量分别为87、69、60 mg/L;从每组小鼠的12种组织提取总RNA,经Dot blotting检测证明,三种重组载体除在乳腺中表达外,p205C3LYZ还在小鼠的脾和肠、pBJLYZ在脾、pBCLYZ在脾和肾中具有一定的异位表达;在有hLYZ mRNA转录的上述脏器中用微球菌溶解试验均可检测到hLYZ活性。

From Maxwell's equations, with material constitutive relation of optically active crystal and boundary conditions of electric field on the boundary between isotropic media and crystal, through coordinate transformation, the formula of the direction of refracted wave for arbitrary direction incident monochromic plane wave was derived. Furthermore, based on the unit vector of direction of refracted wave, eigen waves of electric field, electric displacement vectors and direction of energy flow can be easily obtained.

从麦克斯韦方程组出发,结合旋光晶体的物质方程以及单色平面波从各向同性介质入射到晶体界面时电场的连续性边界条件,通过晶体主轴坐标系和界面坐标系的坐标变换,推导了任意入射方向的单色平面波在晶体界面上的折射方向的求解公式,进而利用所得的折射方向求解晶体中折射波的电场本征模、电位移矢量本征模和光线方向。

Herpes simplex virus HSV -based vectors have primarily been developed for neuronal gene delivery , taking advantage of the virus 39 natural neurotropism .

单纯疱疹病毒为基础的载体主要是发达国家的神经元基因传递,利用病毒的自然神经性。

All of these results could found the bases for study on germline transformation of noctuidae,and for further study on construction of highly effective gene-transfer vectors.

这一结果为夜蛾类昆虫的转基因研究,以及开发新的更高效的piggyBac转基因载体,奠定了坚实的基础。

After being digested with EcoR I and Xho I, they were inserted to the vectors pGEX-4T-l orientally, and then transformed into E.coli BL21(DE3) cells. The monoclone were selected and identified by means of restriction analysis, PCR and sequencing. As the result, the two recombinant plasmids with the different DNA fragements of the PrP gene were constructed and designated as pMY01[Ov rPrP(23-244)] and pMY02[Ov rPrP(91 -244)], respectively.

挑选单个菌落,提取重组质粒,经酶切、PCR扩增后琼脂糖凝胶电泳分析和测序鉴定,成功地构建了2种绵羊重组朊蛋白的表达质粒pMY01[OvrPrP(23~244)]和pMYO2[Ov rPrP(91~244)]。

In this study, an avirulent gene avrD (0.93 kb) isolated from a pathogenic bacteria Pseudomonas syringae PV. tomato and another avirulent gene Elicitin (0.294 kb) isolated from a pathomycete Phytophthora parasitica were ligated respectively with the non-specific pathogen induced promoters Pill and BG to construct plant expressive vectors pYH144 and pYHEt containing the two chimeric genes (Pill-avrD, BG-Elicitin).

tomato中获得的无毒基因avrD (0*93 kb)和从病原真菌Phytophthora parasitica中获得的无毒基因Elicitin(0.294 kb)分别与非专一性病原物诱导启动子Pill和BG组成含2个嵌合基因(Pill-avrD, BG-Elicitin)的植物表达载体pYH144和pYHEt。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。