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We developed the recombinant green fluorescent protein gene yeast cell to screen estrogenic compounds based on two episomal vectors.

以载体双表达的方式构建重组酵母环境雌激素的评价体系，用于快速筛选雌激素类化合物。

Wake velocities at the propeller disk plane, other than pressure coefficient and skin friction coefficient, are given, and vortices structures shed from the fairwater and four stern appendages are observed from the velocity vectors.

同实测结果进行了对比，相当吻合。

The XYN7 gene, located on multicopy shuttle vectors pYES6/CT, was successfully expressed in the yeast Saccharomyces cerevisiae under the control of the GALI promoter and CYC1 transcription termination signal , respectively.

XYN7基因构建在多拷贝穿梭载体pYES6／CT上，在GALI启动子和CYC1转录终止信号的控制下成功在酿酒酵母中表达。

Four chlorite geothermometers, which are universally applied, are introduced and compared in this paper. Main effort has been devoted to discussing the necessity of Fe/ adjustment on chlorite geothermometer by reviewing ion substitution vectors and by comparing calculation errors.

介绍了四种应用最为广泛的绿泥石成分温度计的原理，并从离子替代规律和比较研究的角度着重讨论了近年来关于绿泥石成分温度计校正的必要性。

The invention clones geranyl pyrophosphate synthase GGPPS gene from the ginkgo to construct plant expression vectors which contain ggpps gene, the ggpps gene is inducted into immature embryos of the ginkgo to induct out the callus tissue by the mediating of agrobacterium tumefaciens, PCR and semiquantitative RT-PCR detect the conformation and expression status of exogenous target gene ggpps, high-performance liquid chromatography and an evaporative light-scattering detector are employed to determine the terpene lactones content inside the callus tissue of the ginkgo, and the obtained callus tissue of the ginkgo of which the terpene lactones content is increased is screened.

本发明从银杏中克隆香叶基香叶基焦磷酸合成酶GGPPS基因，构建含ggpps基因的植物表达载体，用根癌农杆菌介导，将ggpps基因导入银杏幼胚并诱导出愈伤组织，PCR和半定量RT-PCR检测外源目的基因ggpps的整合和表达情况，高效液相色谱法及蒸发光散射检测器测定银杏愈伤组织中萜内酯含量，筛选获得银杏萜内酯含量提高的转基因银杏愈伤组织。

Was used to cons Methods: The pSUPER plasmid cloned into RNA polymeraseⅢ H1 promoter was used to construct new vectors which can code hereditable shRNA aimed to HPV18E6/E7 gene mRNA.

采用克隆有人Ⅲ型RNA聚合酶H1启动子的pSUPER质粒，设计并构建针对HPV18E6、E7基因mRNA的、可稳定遗传的编码发夹状结构的特异性RNA干扰质粒载体。

We demonstrated that recombinant baculovirus vectors expressing p35 (Ac-CMV-p35) and eGFP could be transduced into human kidney 293 cells efficiently. The level of transgene expression was viral dose dependent and high-level expression of the target gene could be achieved under the heterogonous promoter.

本研究证明重组杆状病毒载体Ac-CMV-p35和Ac-CMV-GFP能有效地转导人胚肾293细胞，并介导外源基因高效表达，目的基因的表达水平与杆状病毒的感染剂量成正线性相关。

In order to meet the different needs of heterologous gene expression, we go on to construct the pHR series vectors.

为了使构建的载体能适应不同类型的外源基因的表达需要，进一步构建了pHR系列载体。

The pCS vectors has been shown to be useful tools for cloning and expression of heterologous genes in thermophilic hosts.

所构建的pCS系列载体为在高温细菌宿主中进行外源基因的克隆和表达提供了有效的手段。

Both vectors were stable in host cells tested and could be generally useful for expressing heterologous genes.

两种载体在宿主细胞中是稳定的，应可用于异源基因的表达。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。