查询词典 vectors

To clone promoter-active fragments from Corynebacterium glutamicum for further construction of expression vectors.

获得谷氨酸棒杆菌10147基因组中具有启动子活性片段的结构序列，为构建表达载体做准备。

Methods The IL17 cDNA and Thy1.1 fulllength cDNA were subcloned into MIT(MSCVIRESThy1.1) retrovirus vector, and the siRNAIL17, U6 promoter and Thy1.1 fulllength cDNA were also inserted into retrovirus vector of pMNDBANSHEE.The recombined vectors were transfected 293 packaging cells by DNA calcium phosphate coprecipitation. Virus supernatant which infected preactivated spleen cells from NOD/BDC mice was collected. After incubation, the IL17 expression in diabetogenic T cells was detected.

利用基因工程和细胞克隆技术分别将IL17的cDNA插入MSCVIRESThy1.1逆转录病毒载体，将Thy1.1、U6增强子(U6 promoter)基因和IL17的siRNA cDNA插入逆转录病毒载体pMNDBANSHEE，采用磷酸钙沉淀法将重组载体转染293包装细胞；收集病毒上清，转染预先活化的NOD/BDC小鼠脾细胞，测定致糖尿病性T细胞的IL17和siRNAIL17的表达。

SMMC-7721 cells transfected with antisense DNMT1 (DNA methyltransferase Ⅰ) gene fragment (SMMC-7721 aDNMT1) was more sensitive to TRAIL than SMMC-7721 cells transfected with sense DNMT1 gene fragment or vectors without any ectogenous gene fragment.

4MTT法和TUNEL法测得的结果显示，肝癌SMMC-7721细胞转入反义DNMT1基因片段后对TRAIL更加敏感。

This review focuses on the development and means of the modification of polymeric gene vector making them have multi-function—the ability to maintain stability outside of cell、the ability of cell entrance、the ability of endosome escape and the ability of nuclear membrane traversing, at last, we highlight the main challenges that remain and the future direction of polymeric vectors research.

本文在分析基因治疗过程的基础上，详述了基因载体应具有的四种基本功能——在细胞外保持稳定的功能、进入细胞的功能、逃离溶酶体的功能、进入细胞核的功能，并详细介绍了有关基因载体功能化修饰的方法和目前国际上的进展情况，最后本文总结了高聚物载体目前存在的主要问题及未来的发展方向。

Thus,CMG-KALA nanoparticles have great potential as gene vectors for transfection in vitro.2.GS-peptide nanoparticles were synthesized as previous reported method in order to improve the ability of cell penetrating,endosome escaping and nuclear targeting.

通过激光共聚焦、荧光光谱以及红外光谱证明KALA可以成功地连接到CMG纳米颗粒表面；通过静电引力吸附将DNA与CMG-KALA纳米颗粒进行复合，并通过琼脂糖凝胶电泳证明该纳米颗粒对DNA具有很好的保护作用。

To investigate the role of gene Gli3 in idiopathic congenital talipes equinovarus, we constructed the Gli3 luciferase reporter gene expression vectors to analyze the promoter activity of the rat gene Gli3. The regulatory element in the promoter region of the rat Gli3 was predicted using P-Match software and further verified by ChIP experiment.

为了探讨人类GLI3基因在单纯性马蹄内翻足(Idiopathic congenital talipes equinovarus， ICTEV)发生时所起的作用，文章构建了大鼠Gli3基因启动子区域荧光素酶报告基因表达载体来分析Gli3基因启动子的活性，并利用P-Match软件预测大鼠Gli3基因启动子区域可能的调控元件，应用ChIP实验加以验证。

The process of feature extraction is to transform the eradiate noise signal to different feature space and extract the feature vectors that reflect the category of the input sample. The extracted features are the input modes to the classifier. Through the inspecting, the author selected three methods of eradiate noise's feature extraction.

本文作者经过考察，采用了三种前期研究中较为有效的水下目标特征提取方法——基于功率谱估计的线谱特征提取方法、基于小波分析的不同频段内能量特征提取方法和基于水声信号分维特性的特征提取方法。

This is, further to certify that the parent plants were grown on the farm in which the intensive controls against the vectors of Erwinia stewartii were carried out, and these plants were inspected on the field during the most active growing season and found to be free from the pest mentioned above.

（兹证明母本植物种植地已针对玉米细菌性枯萎病菌媒介采取严密的防控措施，并在这些植物最具活力的生长季节进行田间检查，未发现玉米细菌性枯萎病菌。

To modify the structures of erythromycin analogs in S. erythraea, stable expression vectors need to be constructed.

为了对红霉素类似物进行结构修饰研究，需要能够在糖多孢红霉菌中有效表达外源片段的载体。

To study the regulation mechanism of SOCS-3 (Suppressor of cytokines signals-3) for erythropoietic development, small interference RNA expression vectors of SOCS-3 were constructed and transferred in to the K562 cell lines stably by lentiviral system.

为了研究细胞因子信号转导分子3(suppressor of cytokine signals-3，SOCS-3)对造血发育的影响，构建了SOCS-3慢病毒siRNA干涉载体，并转染人红白血病细胞株K562。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。