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Subsequently, on the basis of the system, the vectors pBar-1C and pBar-1Ab were transformed. 156 and 115 of resistant calli were obtained from 5400 and 4800 calli, respectively. Their transformation efficiency were 2.8% and 2.4%, and differentiation rates were 80.7% and 87.8%, respectively. These results suggest the transformation system is stable as well as efficiency, and good for transformation of rice with Bar gene as selective marker.

随后,以此体系为基础,将装有不同基因的两个载体pBar-1C和pBar-1Ab进行了转化,分别从5400块和4800块愈伤组织中获得156个和115个抗性愈伤,其抗性愈伤率分别为2.8%和2.4%,分化率分别为80.7%和87.8%,由此说明,此转化体系相对稳定,且分化率高、抗性愈伤率也较高,可用于同类选择标记的遗传转化,加快水稻转基因育种步伐。

HCV core antigen and TMV CP were detected in the systemically infected tobacco by ELISA and Western blotting . Similar expression of HCV core antigen on serial passages suggested that the complementation system of TMV-based vectors was stable in systemic infection and expression.

通过比较它们在侵染叶片中HCV核心抗原的积累水平,我们发现TMV外壳蛋白基因的5,端序列具有增强其表达的功能,其作用机制是它的起始区IV比起始区V具有更强的起始翻译功能。

In the algorithm, tangent plane of the surface at each point is first calculated according to the point and its neighbor points. In an optimized sequence, normal vectors of the tangent planes are oriented to the outside of the surface. Finally, marching cube method is used to output the triangular representation of the surface.

该算法首先根据测点的邻近测点估算曲面在该测点处的法矢,并采用优化的顺序对法矢方向进行调整以使各测点处的法矢都指向曲面外侧,最后用步进立方体算法输出三角网格模型。

The book takes a fresh approach to tensor analysis built solely on the metric and vectors, with no need for one-forms.

那些书花费新通路给仅仅关于米制和矢量建造的tensor 分析,没有适合一-形式需要。

Based on the structural relationship of quadrilaterals on a scalp and the interpolation of texel starting vectors, we further modify and improve the simple hair styles.

然后根据头皮曲面上四边形的结构关系和纹元起始向量的插值方法,将简单造型予以修正和发展,给出几个发型的构造方法和实例。

Then the promoter CaMV 35S driven, plant siRNA expression vector pARB with inverted repeats of BBE was constructed based on the vectors pHANNIBAL and pART27. The work will lay the foundation for breeding a low morphine and high thebaine poppy.

以中间载体pHANNIBAL和植物表达载体pART27为基础,构建了CaMV-35S启动子驱动的含小檗碱桥酶基因片段反向重复序列的RNAi双元表达载体pARB,为培育低吗啡,高蒂巴因的罂粟新品系奠定了基础。

Then the promoter CaMV 35S driven, plant siRNA expression vector pARB with inverted repeats of BBEwas constructed based on the vectors pHANNIBAL and pART27. The work will lay the foundation for breeding a low morphine and high thebaine poppy.

以中间载体pHANNIBAL和植物表达载体pART27为基础,构建了CaMV-35S启动子驱动的含小檗碱桥酶基因片段反向重复序列的RNAi双元表达载体pARB,为培育低吗啡,高蒂巴因的罂粟新品系奠定了基础。

The cloned BBE gene sequences were 94.84% identified with the reported BBE genes in GenBank previously. Based on the cDNA sequences of COR and BBE ,two fragments about 400~500 bp from each gene with lower identity among them were cloned. The fusion gene BC (744 bp) is fused by the PCR technique. Then the promoter CaMV 35S driven, containing'forward BC fusion fragments-reverse pdk intron-reverse BC fusion fragments', plant siRNA expression vector were constructed based on the vectors pHANNIBAL and pART27.The work will lay the foundation for breeding a low morphine and high thebaine poppy.

利用blast及分子生物学软件DNAStar对COR和BBE基因的cDNA序列同源性进行分析比较,分别从各基因中筛选和克隆了一段同源性极低、约400~500 bp的片段;并应用重叠PCR法将其拼接成744 bp的融合基因BC,以中间载体pHANNIBAL和植物表达载体pART27为基础,构建了以CaMV 35S启动子驱动的含有"正向BC融合片段- pdk内含子-反向BC融合片段"的ihRNAi植物表达载体,通过转化野生罂粟,初步研究了以COR和BBE基因为靶标的RNAi对内源吗啡合成的抑制效果,为进一步培育低吗啡高蒂巴因的罂粟种质提供了依据。

Thus an effective reduction algorithm for document vectors are indispensable, while those traditional algorithms based on frequency and threshold filtering may often lead to the loss of many effective information.

而传统的基于频率的阈值过滤法往往会导致有效信息的丢失,也影响了分类的准确度。

The main problem of direct toque control of a three-level inverter and control technique virtual synthesizing vectors based are demonstrated in detail, also, the method of vector selection is discussed materially.

深入阐述了三电平逆变器直接转矩控制的主要问题及虚拟合成矢量控制方法,详细讨论了三电平逆变器直接转矩控制中的矢量选择方案。

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We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。