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The article introduces two arithmetic of partial differential,and analyses the method of using compare the current vector of coupler breaker,and provides sampling arithmetic and vector arithmetic.

母线微机保护装置已发展的比较成熟，装置通用的选择故障母线的方法是利用总差动和分差动相配合；文中介绍了利用分差动选择故障母线时的两种算法，分析了利用母联电流方向比较元件选择故障母线的方法，并从瞬时值方面和向量值方面提供了两种算法。

Basing on the theory on Space-Vector PWM, the paper describes the mapping implemented by Neural-Network from tracked voltage vector to turn-on time of IGBT about rectifying sect of direct-driven synchronous generator excited by permanent magnets convertor system.

本文针对直驱永磁风力发电机变流系统的整流部分，利用空间矢量脉宽调制的原理，利用神经网络来实现跟踪的电压矢量到整流器三相桥臂开关时间的映射。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

But the algorithm involves big matrix computation except the scaling coefficient vector and the wavelet coefficient vector at the coarsest scale.

不足之处是较大尺度的小波系数较长，存在较高维数的向量和矩阵运算，计算量大。

A codebook is used for feedbacking power distribution vector, which can effectively reduce the feedback amount of information; the use of two codebooks are used at the same time, which can respectively feedback the power distribution vector and pre-code matrix and can improve the transmission performance of the system under the condition of limited feedback.

使用码本反馈功率分配矢量，可以有效地减少信息的反馈量。同时使用两个码本，分别反馈功率分配矢量和预编码矩阵，可以在有限反馈的条件下提高系统的传输性能。

Connective reaction: 6.5μl PCR productions, pMD18-T vector 0.5μl, Ligation SolutionⅠ5μl, T4 DNA Ligase 0.5μl, 10×T4 DNA Ligase 1.5μl were connected overnight at 16℃. 5、transformation of vector: DH5α was prepared by CaCl and mixed with connecters, cultured on Amp+LB overnight at 37℃.

双酶切及PCR鉴定。1%琼脂糖凝胶电泳。7、序列测定：测序结果与Gene Bank中报道的序列同源性为99.1%，说明变链ldh基因及同源区基因已克隆成功；命名该重组质粒为pMD18-T-ldh。

This research selected the strong functional Prokaryotic expression vector pET32a+ and Eucaryote expression vector pMET α A.

通过选用功能强大的原核表达载体pET32a+和真核表达载体pMET α A，构建了Ecoli。

Full length LMP2A cDNA was firstly incised from pGEM-T-LMP2A with EcoR Ⅰ, Sma Ⅰ digestion, and then inserted into eucaryote. expression plasmid pCIcc controlled by CMV promoter. The CMV-LMP2A-SV40 expression unit was digested by ClaI, and inserted into E1-substituted adenovirus vector pAx1cw. Then the LMP2A recombinant adenovirus vector was cotransfected into 293 cells together with EcoT22I digested Ad5-TPC.

将带有LMP2A cDNA的重组质粒pGEM-T-LMP2A用EcoR Ⅰ、Sma Ⅰ双酶切下LMP2A cDNA，并将其插入含同样酶切位点的真核表达质粒pCIcc中，使其受控于CMV启动子下；用ClaI切下CMV-LMP2A-SV40表达单元，插入E1、E3区替代的腺病毒载体pAX1CW，选择正确的克隆pAX1CW-LMP2A与Ad5 DNA-末端肽复合体共转染293细胞，通过同源重组获得复制缺陷型的重组腺病毒(Ad5-LMP2A)。

Taking the pcDNA 3.1 expressions vector as the frame structure, we try to set up a eukaryon expression vector.

并以pcDNA3.1表达载体为框架结构，尝试构建真核表达载体。

After inserting the mutant fluorescence protein gene into the vector, the novel vaccinia virus vector p16H1FP and p16H2FP to express proteins as fusion products containing an additional amino-terminal stretch of six histidines were constructed.

我们用PCR扩出N-端融合6×His·Tag多克隆位点基因序列，插入p16表达载体，并在其多克隆位点下游装入带有巨细胞病毒启动子的突变型荧光蛋白基因，构建成两个读框的N-端融合6×His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。