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The recombinant vector was constructed by subcloning SV40LT gene (2.1 Kb) from SV40LT gene-carrying plasmid pLLTSN into the BamH I and Xho I sites of pantropic retroviral expression vector pLXRN.

从携带SV40LT基因的质粒pLLTSN中高保真PCR扩增出2.1 Kb的SV40LT基因，将其插入到泛嗜性逆转录病毒载体pLXRN的BamHⅠ和XhoⅠ位点之间，构建重组载体pLXRN-SV40LT。

Tuberculosis H37Rv strain was amplified by PCR and cloned to vector pEASY T1. After sequencing, it was subcloned to fusion expression vector pET28a and then transfected to E coli BL21 (DE3). It was found that the LprG protein was successfully expressed after induction with LPTG.

采用聚合酶链反应从结核分枝杆菌H37Rv基因组中扩增出LprG基因，克隆到pEASY T1中，序列测定正确后，再亚克隆到融合表达载体PET28a中，转化入大肠杆菌BL21(DE3)，经IPTG诱导表达。

Chem. Chem. Phys 2000, 2: 613, Chem. Phys, 2000, 259: 99. The results show that the product rotational angular momentum vector is not only aligned, but also along the y-axis perpendicular to the k-k' scattering plane on both PESs, orientation of product angular momentum vector depends on collision energies and PESs.

结果表明在两个势能面上产物的转动角动量矢量不仅取向，而且沿着y轴垂直于散射平面定向，产物的角动量定向依赖于碰撞能和势能面。

Methods- Full length HBsAg cDNAs were subcloned into pIND vector , followed by being cloned into shuttle2 vector.

将HBsAg基因亚克隆到pIND载体和Shuttle2载体中，构建穿梭载体Shuttle2-S。

Methods: Full length AFP cDNAs were subcloned into pIND vector, followed by being cloned into shut- tle2 vector.

将AFP基因亚克隆到pIND载体和Shuttle2载体中，构建穿梭载体Shuttle2-AFP。

Conditional rBDNF transgenic vector was successfully constructed and strong overexpression of rBDNF and EGFP were identified in the cotransfected cell line, and establishment of this targeting vector is critical for pronuclear microinjection.

rBDNF条件性基因载体构建成功，在体外细胞系中，检测到rBDNF及EGFP强表达，为转基因显微注射打下基础。

The protozoa were isolated from field animal blood, vectors, pathogenic tissue and secretions. Based on differences of the pathogen morphology, vector species, experimental infected animals, single parasite clone and parasitic tissues, protozoiasis pathogens from different areas and animals in China were isolated and the vectors and transmission mode were also determined by vector transmission trial. Sequence analysis of 18S rRNA gene was used to study the phylogeny of partial isolated protozoa.

自野外采集动物血液、传播媒介、相关组织、排泄物等，根据对病原形态鉴定、传播媒介鉴定、实验性感染易感动物、单一虫株克隆、不同致病部位等方法，对我国不同地区、不同家畜的原虫病病原进行分离，并通过媒介传播试验确定传播媒介的种类与传播方式；采用18S rRNA基因测序方法，对本实验室已分离的部分原虫进行进化关系研究。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

LP Recco PCR Cloning can efficiently and conveniently construct high quality prokaryotic expression vector of human NY-ESO-1 gene, which provide the expression vector to produce pET-15b-NY-ESO-1 fusion protein.

靶向克隆法可快速、高效地构建人NY-ESO-1基因的原核表达载体，为制备pET-15b-NY-ESO-1融合蛋白奠定了基础。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。