查询词典 vector

The CryCI gene and essential regulation elements cut from the plasmid pGF4ABC were inserted into yeast expression vector pPIC9K and plant expression vector pBI 121.1, so we got the secretive yeast expression plasmid pPIC9KBC and plant high-efficiency expression plasmid pGBIF4ABC, Fifty-two His+Muts transformants were obtained after the expression vector pPIC9KBC was introduced into Pichia pasoris, KM7I strain, by electroporation.

将pGF4ABC上的融合杀虫基因CryCI分别克隆到酵母表达载体pPIC9K和植物表达载体pBI121.1上，构建成融合杀虫基因的分泌型酵母表达载体pPIC9KBC和高效植物表达载体pGBIF4ABC。

It points out that under the hard-switching PWM pattern, the movement speed of flux linkage is adjusted by zero space voltage vector, but when soft-switching PWM pattern is used, effect time of space voltage vector vary greatly, sometimes even no zero space voltage vector exist.

指出在硬开关PWM模式下，磁链的运动速度靠零空间电压矢量调节；而在软开关PWM模式下，空间电压矢量的作用时间发生了很大变化，有时甚至没有零空间电压矢量。

A new SVM method suitable for four-switch inverter fed PMSM DTC system was proposed. By analyzing the property of the equivalent zero space vector and its imitation scheme in detail, the paper introduced a zero-vector partition idea, which is usually adopted in the SVPWM modulation, to the composition of desired space vector.

该文针对四开关逆变器供电PMSM DTC系统的特殊性，采用了一种新型SVM方法，分析了等效零矢量模拟技术，将传统SVPWM调制中的劈零思想引入到空间矢量的合成上。

Experimental results show that the zero effort miss vector calculated by using approximate formulas is close to the real miss vector. Under the conditions of small initial errors, the satisfied results can be obtained by approximate function. The zero effort miss vector obtained can be used for the preliminary design and analysis of kinetic interceptor guidance.

测试结果表明该近似算法得到的零控脱靶量与真实值比较接近，在较小初始偏差条件下，简化的近似计算公式能给出满意的结果，所得零控脱靶量可用于空间动能拦截制导系统的初步设计与分析。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

This algorithm constructs a 64-D crossband vector according to the characteristics of the decomposed image, and adopts nonlinear interpolative vector quantization and soft computing based codebook generation algorithm. Hence, this algorithm can improve the vector quantization performance significantly from both aspects of speed and precision, and it can get a high reconstruction quality (PSNR＞34dB) with a very low bit rate (0.172bpp).

该方案依据图象小波变换的特点构造了64维的跨频带矢量，并采用了非线性插补矢量量化和柔性计算码书生成算法，从速度和精度两方面提高矢量量化的性能，从而在极低比特率的条件下(0.172bpp)获得了高质量的重构图象(PSNR＞34dB)。

According to the classical signal transduction pathway of estrogen, we use Luciferase reporter gene to screen phytoestrogen form Selaginella tamariscina, Pinus massoniana Lamb, Corallodiscus flabellate, Dryopteris sublaeta Ching et Hsu and Leonurus heterophyllus. In this study,the pβgal-Control vector, the pERE-TAL-luc vector, the pCNX2-hERα/pCNX2-hERβvector were transfected into HEK293 cell line. If the components in the herbs combine with the ER, then it could induce the expression of reporter gene controlled by ERE.

根据体内的雌激素信号转导途径，我们选用了以luc为报告基因、ERE为调控序列的重组报告基因载体pERE-TAL-luc，并将其与重组人ERα(humanERα，hERα)表达载体pCXN2-hERα和重组人ERβ(humanERβ，hERβ)表达载体pCXN2-hERβ共转染ER阴性细胞系，建立了靶向ERα或ERβ的细胞水平药物筛选模型，结合小鼠子宫增重实验，对卷柏、松针、益母草、石胆草及贯众等5种中药的植物雌激素作用进行了研究，以期为临床防治更年期综合症等提供准确、科学的实验基础。

The three conserved histidine residues with a conserved glutamate residue fits the active site of the homology model of oxalate oxidase.2. Construction of plant binary expression vector of germin geneThe germin gene fragment cut with BamH I from intermediated vector pDG28 was inserted into binary vector pBI121 cut with the same enzyme to formrecombinants which was selected by plaque PCR .

Germin基因植物表达载体的构建将中间载体上的germin基因片段用Bam H I酶切下来，插入用同样酶切的双元表达载体pBI121上，以菌落PCR筛选重组子。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。