查询词典 vector-potential

LP Recco PCR Cloning can efficiently and conveniently construct high quality prokaryotic expression vector of human NY-ESO-1 gene, which provide the expression vector to produce pET-15b-NY-ESO-1 fusion protein.

靶向克隆法可快速、高效地构建人NY-ESO-1基因的原核表达载体，为制备pET-15b-NY-ESO-1融合蛋白奠定了基础。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

Based on the statistic feature of block matching moving vector, the method calculates the basic translation vector between current frame and referenced frame through a revisable parameter, and adaptively chooses processing algorithm for image sources with different characteristics.

该方法从分析块匹配运动矢量统计特性出发，通过引入一个可预设置参数，计算当前帧与参考帧之间的基本平移矢量，并自适应地采取混合全局运动、局部运动估计的算法处理不同特征的源数据。

I will show systematically how we induce the kernel functions of support vector machine to the Riemannian metric,which makes possible for studying the support vector machine in geometry.

系统的 讲述了SVRM问题、如何构造SVRM的核函数和B样条核函数的几何修正。在这章我们详细的介绍了怎样来用支撑向量回归机解决拟合或逼近问题。

This paper first discusses the nature of the kernel function,and then based on Riemannian geometry dependent on the data structure and method,an improved modifying kernel function is proposed,the kernel function is a simple and less calculation method,which conformal factor and support vector irrelevant,compared with previous studies overcomes the impact of support vector's number and distribution.

首先讨论核函数的本质，并且基于黎曼几何结构和数据依赖的方法，提出了一种改进的修正核函数，改进后的核函数形式简单，计算量较低，其中保形因子与支持向量无关，较之于以前的研究克服了支持向量的数目和分布的影响。

As a cellular vector, orally-administrated attenuated salmonellae could transfer target genes eukaryotic expression vector into stomach and intestinal tissues for gastric ulcer treatment in rats.

在大鼠胃溃疡治疗中，减毒沙门氏菌作为细胞载体以po方式可将携带目的基因的真核表达载体相对靶向性转入胃、肠组织中。

Shandong sinder technology limited company,qingdao 266061,shandong,chinaabstract:to provide the eukaryotic expression vector carrying 6x histidine-tag and human growth hormone in expremental animal and cell culture,according to the published nucleotide sequence of the lactofericin b and human growth hormone,synthesis sequence was ligated with pmt-18 vector.

以牛乳素为目的基因，构建含有组氨酸标签、人生长激素信号肽的真核表达载体，为利用牛乳素在活体动物及培养细胞中的表达提供基因材料。

To prepare recombinant fox growth hormone, we amplified its cDNA from silver fox pituitary tissue by RT-PCR and cloned into yeast shuttle vector pPIC9K down stream of α-factor signal peptide sequence by SnaB I and Not I restriction sites. The recombinant secretion vector pPIC9K/fGH, linearized by Sal I, was transformed into histidine-deficient Pichia pastoris strain GS115 by electroporation.

为制备重组狐狸生长激素，采用RT-PCR方法，从银狐垂体中扩增fGH cDNA基因，利用SnaB I和Not I位点将fGH基因插入到酵母分泌型表达载体pPIC9K中α-因子信号肽的下游，构建成fGH基因的酵母分泌型表达载体pPIC9K/fGH，载体经Sal I酶切线性化后，通过电转移将线性化的pPIC9K/fGH转化到组氨酸缺陷型酵母宿主菌GS115中。

The thesis illustrates the principle of the and its application in the system, establishes the simulation model of the vector control system of PMSM based on the EKF and successfully realizes the application of the speedless sensor in motor control. Firstly, based on the mathematic model of permanent-magnet synchronous motor, the PMSM vector control system is built. Secondly, the Extended Kalman Filter is designed for the on-line estimation of the speed and rotor position by only using measurements of the motor voltages and currents.

首先，基于PMSM的数学模型，建立了电机的矢量控制仿真模型；其次，设计了扩展卡尔曼滤波器，通过检测电机端电压、相电流对电机转速和转子位置进行在线实时估计，实现了PMSM的无传感器矢量控制；最后，在Matlab7.0环境下进行的系统仿真试验表明，该方法具有优良的转角跟踪特性和较好的转速跟踪特性，同时系统具有较强的抗负载扰动性能和较佳的控制性能。

The open reading frame of Spinacia oleracea Betaine Aldehyde Dehydrogenase was retrieved from Spinacia oleracea and inserted into the Agrobacterium tumefaciens binary vector pBin438, which was driven by CaMV35S promoter, and produced the new binary vector pBSB. A. tumefaciens LBA4404 carrying this plasmid was used in genetic transformation of plants. Forty-five primary transgenic plants were detected by PCR and verified by the Southern blotting from 65 regenerated plants, of which 27 transgenic plants had only one copy of T-DNA.

摘 要：分离出菠菜甜菜碱醛脱氢酶基因构建成由CaMV35S驱动的双元植物表达载体pBSB，农杆菌菌株LBA4404携带该载体转化棉花，获得转基因棉花植株。65株转基因植株经过PCR筛选、Southern blotting分析证明有45株为成功的转化株，外源基因已经被整合到棉花的染色体组中，并以单拷贝插入居多。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。