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The nanoplex were smaller than the unmodified nHAP; thelipiodol/nanoplex emulsion, with the mean diameter at 75±11.8nm can disperse moreuniformly and be more soluble and stable, contrary to the unmodified nHAP and nanoplex Conclusion: Nanoplex/pDNA mass ratios greater than 15 had exhibited completepDNA absorption onto positive charged polyplex and pDNA protection from thedestruction of nucleinase in rabbit serum. Unmodified nHAP may can\'t induce effectivegene delivery.PartⅡThe construction of the recombint eukaryotic expressive vector ofwild-type human p53 and RbObjective: To construct the recombint eukaryotic expressive vector of wild-type humanp53 and Rb by utilizing the theory of molecular medicine.

结果nHAP颗粒本身大小小于100nm,但由于颗粒表面本身带负电,不能包括有效地结合及保护DNA;经Ca~(2+)修饰后,颗粒可带正电,可一定程度地结合和保护DNA,但聚合物本身的中性电与带负电的细胞膜结合,而且本身的颗粒则变地容易聚集而成为很大的颗粒,也不利于细胞的吞噬;经PLL修饰后,颗粒溶解性提高,直径变小,表面带正电,电泳结果显示PLL-nHAP颗粒可包括有效地结合和保护DNA免受兔血清的破坏,而且复合物表面的正电也包括有利于与细胞膜的结合与吞噬。

This purpose of design is a computer simulation ventricle except that electric vector quantity of the heart surrounds the running orbit very much, through the stereoscopic and dynamic way, show the course that the vectorial ring of electricity of heart forms, can make student image, understand electric vector quantity of the heart ocularly; Type the well practical foundation in order to study the kind electric picture.

本设计目的是计算机模拟心室除极心电向量环运行轨迹,通过立体动态的方式,展示心电向量环形成的过程,使学生能够更形象,直观地理解心电向量;为学好心电图打好切实的基础。

The recombinant vector was constructed by subcloning SV40LT gene (2.1 Kb) from SV40LT gene-carrying plasmid pLLTSN into the BamH I and Xho I sites of pantropic retroviral expression vector pLXRN.

从携带SV40LT基因的质粒pLLTSN中高保真PCR扩增出2.1 Kb的SV40LT基因,将其插入到泛嗜性逆转录病毒载体pLXRN的BamHⅠ和XhoⅠ位点之间,构建重组载体pLXRN-SV40LT。

Tuberculosis H37Rv strain was amplified by PCR and cloned to vector pEASY T1. After sequencing, it was subcloned to fusion expression vector pET28a and then transfected to E coli BL21 (DE3). It was found that the LprG protein was successfully expressed after induction with LPTG.

采用聚合酶链反应从结核分枝杆菌H37Rv基因组中扩增出LprG基因,克隆到pEASY T1中,序列测定正确后,再亚克隆到融合表达载体PET28a中,转化入大肠杆菌BL21(DE3),经IPTG诱导表达。

Chem. Chem. Phys 2000, 2: 613, Chem. Phys, 2000, 259: 99. The results show that the product rotational angular momentum vector is not only aligned, but also along the y-axis perpendicular to the k-k' scattering plane on both PESs, orientation of product angular momentum vector depends on collision energies and PESs.

结果表明在两个势能面上产物的转动角动量矢量不仅取向,而且沿着y轴垂直于散射平面定向,产物的角动量定向依赖于碰撞能和势能面。

Methods- Full length HBsAg cDNAs were subcloned into pIND vector , followed by being cloned into shuttle2 vector.

将HBsAg基因亚克隆到pIND载体和Shuttle2载体中,构建穿梭载体Shuttle2-S。

Methods: Full length AFP cDNAs were subcloned into pIND vector, followed by being cloned into shut- tle2 vector.

将AFP基因亚克隆到pIND载体和Shuttle2载体中,构建穿梭载体Shuttle2-AFP。

Conditional rBDNF transgenic vector was successfully constructed and strong overexpression of rBDNF and EGFP were identified in the cotransfected cell line, and establishment of this targeting vector is critical for pronuclear microinjection.

rBDNF条件性基因载体构建成功,在体外细胞系中,检测到rBDNF及EGFP强表达,为转基因显微注射打下基础。

The protozoa were isolated from field animal blood, vectors, pathogenic tissue and secretions. Based on differences of the pathogen morphology, vector species, experimental infected animals, single parasite clone and parasitic tissues, protozoiasis pathogens from different areas and animals in China were isolated and the vectors and transmission mode were also determined by vector transmission trial. Sequence analysis of 18S rRNA gene was used to study the phylogeny of partial isolated protozoa.

自野外采集动物血液、传播媒介、相关组织、排泄物等,根据对病原形态鉴定、传播媒介鉴定、实验性感染易感动物、单一虫株克隆、不同致病部位等方法,对我国不同地区、不同家畜的原虫病病原进行分离,并通过媒介传播试验确定传播媒介的种类与传播方式;采用18S rRNA基因测序方法,对本实验室已分离的部分原虫进行进化关系研究。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作: 1 回顾了直线电机发展历史,电机的驱动技术演变,特别是针对直线电机的驱动,简要介绍了直线感应电机驱动电梯的优点和不同结构类型,对传统的v/f控制,基于空间矢量法的v/f控制,矢量控制,和基于电压空间矢量的直接转矩控制进行了比较,确立了本课题的研究主题:直线感应电机双边直推式驱动电梯的全数字变频控制。

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Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

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