查询词典 vector-potential

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

We propose a kind of 3D eddy current boundary value problems in the form of two component vector potential and approve the validity the potential in some situations.

本文提出三维涡流场的双分量向量位形式的边值问题，并证明了双分量向量位在一定条件下的适用性。

Assuming that the stratons are heavy and the interaction isdominantly a pseudoscalar potential and a static vector potential,we derive the SU(4)mass splitting for mesons.

在层子质量很重的假定下，考虑相互作用是赝标势和静矢势为主，导出了介子 SU(4)质量分裂公式。

Both components of the perturbed vector potential are considered in the equations, as well as the perturbation of the electrostatic potential.

方程包含了完全的电磁扰动：静电场扰动，磁场的垂直扰动和平行扰动。

In the space without any electricity the static electric field can be described not only by scalar potential ,but also by vector potential.

讨论了在无电荷存在的自由空间，不仅可以用标势描述静电场，而且可以引用矢势描述静电场。

The result is that the magnetic scalar potential method needs less time and less computer memory than the magnetic vector potential method.

结果表明，在计算精度相同的条件下，标量磁位方法比矢量磁位方法耗用较少时间，占用较少的计算机内存。

Transgene expression could be efficiently controlled by using the inducible lentivectors incorporating regulatable system,which expands the potential of lentivectors for a wide array of clinical gene transfer application, therefore it becomes a potential viral vector in gene therapy.

然而，发展应用于临床基因治疗的慢病毒载体，要求能够严格控制目的基因的表达，并确保治疗有效性和减少毒副作用。

The results about the magnetic vector potential of current-carrying ring and the electric potential of uniform charged ring which are expressed by Bessel functions are further calculated and analyzed.

用Bessel函数积分对圆环电流磁矢势及均匀带电圆环电势作进一步的计算分析，引申出磁感应强度及电场强度新的级数表达式，分析讨论了所得结果。

The potential AD patients showed more significant impairment in olfactory function (the average value of DT and RT were respectively 1.970±0.903 and 3.081±0.474) than the controls (the average value of DT and RT were respectively 1.468±0.933 and 2.542±0.748), some of the potential AD patients were anosmia; The DT average value of the controls were significantly lower than the potential AD patients't=2.166, p

本研究中发现可能AD 患者嗅粘膜有与AD 脑内病理改变相关的神经丝堆积，可将其作为研究AD 活的神经细胞的来源。因此怎样不断完善和改进嗅觉检查方法，以进一步提高其早期拟诊AD 的敏感性和特异性，将具有广阔的科研前景和重要的临床应用价值。

Results of cyclic potentiodynamic polarization curve measurement showed that the manufacture of NiTi and stainless steel wires had a significant influence on the breakdown potential, potential difference between breakdown and protection potential, and passive range.

循环动电位极化曲线量测结果显示，镍钛及不锈钢线的厂牌对其崩溃电位、崩溃电位与保护电位差值及钝态区范围均有显著影响；环境的pH值对镍钛及不锈钢线的钝态区范围也有显著影响。

Finally it offers the analysis of the fault-tolerance system as well as its test verification.

这样，运行于空间环境中的系统的设计目标就是在保证系统实时性

However, it is still difficult to find out the real reason of the increase of the keratinized gingiva during the orthodontic extrusion whether it is due to the proliferation of the gingival tissue or to its elastic nature, that is because the proliferation of the fibroblasts as well as the connective tissue changes in volume and the intra-fibers spaces were all immeasurable in most of the studies.

然而，仍然难以找到真正的原因增加的角化牙龈在正畸挤压它是否是由于扩散的牙龈组织或其弹性性质，这是因为增殖的成纤维细胞以及作为结缔组织的变化量和内部纤维空格都是不可估量的，大多数的研究。