查询词典 vector product

Bioassays showed that the product of cry1Aa10 gene was high toxic to Heliothis armigera larvae, indicating that the constructed vector is useful for chloroplast transformation and it will lay a basis for obtaining strongly insect-resistant oilseed rape.

这些结果说明所构建的载体具有可使用性，它为进一步开展油菜的叶绿体遗传转化和获得高抗虫油菜奠定了基础。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

A new eucaryotic expression system of recombinant PoIFN-γ was also constructed so that the denaturalization and refolding process is avoided. The target gene coding PoIFN-γ2 mature protein was subcloned into expression vector, transformed into Pichia pastoris, The His〓 Mut〓 phenotype transformants were screened, fermented in flasks and induced by 1％methanol. The expressed product in culture solution and sedimentation have antiviral activity on VSV and immunological activity proved by indirected immunofluorescence antibody and Dot blotting assay.

为了构建可对表达产物修饰、加工的真核表达系统，使表达产物具有自然干扰素的活性，无需变性、复性，我们将PoIFN-γ2成熟蛋白基因连接酵母整合表达载体，电转化毕赤酵母，筛选了His〓Mut〓表型转化子，摇瓶培养，1％甲醇诱导表达，培养上清和菌体裂解上清均有抗VSV病毒活性，经间接免疫荧光抗体检测和Dot blotting鉴定，重组酵母菌诱导表达产物具有免疫活性。

Objective To study construction of vector to overexpress staphylokinase, expression in vitro and purification of the product.

目的研究高效表达葡激酶载体的构建及其体外表达与产物的纯化。

MethodUsing the canis MC4R DNA as template,the specific primers were designed. After PCR amplification,product was cloned into pGEX-4T-1 vector by LP Recco PCR cloning technique,the recombinant pGEX-4T-1-cMC4R was transferred to E.

方法以Beagle犬基因组DNA为模板，经PCR技术扩增目的片段，利用LP Recco PCR克隆技术将目的片段直接重组到原核表达载体pGEX-4T-1上，转化到E.coli DH5α，筛选阳性克隆，BamHI、XhoI酶切鉴定，DNA 测序检测插入序列的正确性。

To express chicken osteoprotegerin in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro. The chOPG cDNA encoding for 382 amino acid without signal peptice was subcloned into Pichia pastoris expression vector pPICZα-A. The constructed plasmid was transformed into yeast X-33 by electroporation. The recombinant transformants were selected by Zeocin. Induced by the addition of methanol every 24 hours, the product analyzed by SDS-PAGE was sized about 43kDa and 53kDa at a yield of 200mg per litter of culture. The result of Western blotting indicated that the recombinant protein had specific antigenicity mainly owing to heterogeneous glycosylation.

为了在毕赤酵母中分泌表达鸡骨保护素（osteoprotegerin，OPG），采用DNA重组技术将chOPG成熟肽cDNA片段插入毕赤酵母表达载体pPICZα-A中，以电穿孔法转化酵母X-33，用Zeocin 平板筛选重组子，经甲醇诱导表达后， SDS-PAGE 和免疫印迹分析表达产物，由于糖基化不同，表达产物有两种，其相对分子量分别为43kDa和53kDa，表达量约为200mg/L，经Western印迹验证，有较好的抗原性。

To obtain thermostable xylose isomerase, the gene xylA from an extremely thermophilic bacterium,Thermus thermophilus HB8 was cloned and its product was overexpressed in Escherichia coli BL21(DE3) with expression vector pET22b.

为获得具有高热稳定性的木糖异构酶，运用基因工程技术，从嗜热栖热菌Thermus thermophilus HB8中克隆到嗜热木糖异构酶基因xylA。

For 2p〓-periodic binary sequences, a new weight function, according to the above presented vector expression of the linear complexity, is defined. By analyzing the weight of the product of a special kind polynomial in finite field, the upper and lower bounds on minimum error, expressed by the here denned weight of the linear complexity, are given in different cases.

对于2p〓-周期二元序列，利用所给出的线性复杂度的向量表示定义了一种新的重量函数，通过研究有限域上一类特殊多项式乘积的重量，分情况给出了用线性复杂度的重量表示的最小错误minerror的上界和下界，这里的重量是由我们所定义的重量函数确定的。

Then the purification of product was ligated into correspondingly restricted pMD18-T vector.

在含有氨苄青霉素抗性的LB琼脂平板上筛选阳性重组子。

Methods: The total RNA extracted directly from rodent lung tissues which was determined to be hantavirus antigen positive by IFA methos and the hantavirus S gene segment was amplified by RT-PCR methods and the product was purified and cloned into T vector, then the sequence was analyzed.

提取经免疫荧光检测证实为汉坦病毒抗原阳性的鼠肺组织的总RNA，经RT-PCR扩增汉坦病毒S基因片段，其产物纯化后与T载体连接并进行序列分析。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。