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A total of ten legume species were used as materials including Vigna angularis (8 varieties), Vigna radiate (2 varieties), Vigna unguiculata (2 varieties), Phaseolus vulgaris (2 varieties), Arachis hypogaea (2 varieties), Glycine max (2 varieties), Vicia faba (2 varieties), Canavalia gladiata (1 variety), Canavalia ensiformis (1 variety) and Cajanus cajan (1 variety).

试验材料包括红豆(8个品种)、绿豆(2个品种)、豇豆(2个品种)、菜豆(2个品种)、落花生(2个品种)、毛豆(2个品种)、蚕豆(2个品种)、红凤豆(1个品种)、白凤豆(1个品种)及树豆(1个品种),共计10个物种。

Genetic diversity and phylogenetic relationship in goldfish were examined using mitochondrial D-Loop partial sequences at eight representative varieties of goldfishes and wild crucian carp. The eight representative varieties were Red Common Goldfish, Red Fantail, White Oranda with Red Carp, White Pearlscale, Red Tigerhead, Black Moor, Red Moor with Butterfly Tail and Red Bubble-eye with Dorsal Fin. 699 base-pair nucleotide sequences of Mitochondrial D-Loop were examined and analyzed for genetic polymorphism, Sequences data showed that all 129 sequences were grouped into 17 haplotypes. Nine representative varieties shared same haplotype. Five representative varieties of goldfishes had haplotypes respectively.

采用PCR技术扩增了红草金鱼,红文鱼,红顶白高头,白珍珠,红狮头,红龙睛蝶尾,墨龙睛,红水泡八个品种金鱼和野生鲫线粒体DNA的D-Loop区部分序列,PCR产物经纯化、测序、同源序列比对后获得长度为699bp的核苷酸序列,在129个样本中共检测到了17个单倍型,9个品种共享1种单倍型,5个品种的金鱼(BM, MB, WR, RF, RC)检测到各自独有的单倍型。

Breeding improved varieties of tea varieties in Anhui Province in the east field propagation model is the Ministry of Agriculture jointly built seed market, operates mainly in green tea seed and clones (1) green tea has "Gladiolus Spring" brand Xiangshan cloud sharp, middle and low gross peaksà, Gunpowder (2) clonal tea varieties cultivated in recent years, market demand for variety, but also according to customer needs an appointment breeding of all types of varieties.

安徽省东至茶树良种繁殖示范场是属农业部联建的良种场,主要经营绿色食品和无性良种茶苗(1)绿色食品茶叶有"剑兰春"牌香山云尖,中低档毛峰、炒青、珠茶(2)无性良种茶苗培育近几年市场需求的品种,也可根据客户需要预约繁育各种类型的品种。

The accumulating dynamic and differences of 4 husk oat varieties and 5 naked oat variety were studied in this research, and the results showed that the content of β-Glucan of husk oat varieties was higher than that of naked oat varieties, and the content of β-Glucan of Paul which was a naked oat varieties was highest, the MARION was lowest.

本试验通过对4个皮燕麦品种和5个裸燕麦品种β-葡聚糖积累动态和差异性的研究,明确了裸燕麦的β-葡聚糖含量高于皮燕麦,且裸燕麦品种保罗的β-葡聚糖含量最高,MARION的β-葡聚糖最低;在籽粒形成的四个发育阶段中,灌浆后期的β-葡聚糖积累最多对成熟期β-葡聚糖含量有决定性作用。

Positive significant correlation between three infection indexes and SDD, and negative insignificant correlation between infection indexes and LIP were found. Fifteen sugarcane varieties could be divided into 6 groups on the basis of the distance more than 1.0. It resulted most ot tested sugarcane varieties had similar smut resistance. It suggested that smut race 1 and race 2 were included in inocula and the condition for Ustilago scitaminea infection was suitable in the crop season by setting up the standard varieties of NCo310, F134, NCo376 and Ya71-374 for smut identification. In addition, the results of sugarcane varieties resistant to smut race 1 and race2 were known by only a simple field trial.

结果显示:9个引进甘蔗品种中,ROC26属于感病品种,其余属于抗病或高抗品种;3个病情指数和持续发病期的两两相关均为显著正相关,潜伏侵染期与这些参数的相关为负相关,但未达到显著水平;在类间距离大于1.0的条件下,可将15个品种聚为6类,进一步明确了各品种抗黑穗病性的相似程度;抗性鉴定标准对照种NCo310、F134、NCo376和Ya71-374的应用,明确了接种源为小种1和小种2,通过一次接种试验,明确了供试品种对2个小种的抗性水平,标准对照种的抗性表现,还说明了本生长季发病条件基本是适宜的。

Of the 36 tussah silkworm varieties, the Nei's genetic diversity index was 0.3243±0.1515, and Shannon information index was 0.4871±0.1975, suggesting that bivoltine tussah silkworm varieties are still rich in genetic diversity even having been conserved in the same place for quite long time. Nei's genetic identity of the 36 varieties ranged from 0.4959 to 0.8347, 96.59% of which was above 0.6, revealing large similarity between different varieties. A clustering analysis showed that these varieties formed two separate clades, one of which included varieties bred by the institute and the other included imported varieties from other provinces or countries.

供试品种资源的Nei氏遗传多样性指数为0.3243±0.1515,Shannon信息指数为0.4871±0.1975,表明长期同地保存的二化性柞蚕品种资源同样具有丰富的遗传多样性;供试品种的Nei氏遗传一致性范围在0.4959~0.8374之间,达0.6以上的占96.59%,表明多数品种资源的相似程度较高。36份品种资源分别以辽宁省蚕业科学研究所育成种为主和以国内外引进种为主聚为两大类群。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出,等位基因平均值、观察杂合度、Fis值、Fit值皆较高,表明非洲菊等位基因较丰富,杂合基因偏多,且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium;PP8、na、Ⅰ、h、Gi及Fst皆表明,ISSR检测遗传多样性的能力最强,其次是RAPD,等位酶最低;根据遗传距离进行聚类,三种方法都把70个品种分成A、B、C、D、E五个大组,每一组中除少数品种发生位移外,大部份品种分类结果相似,且与形态分析结果有相似性,但在每一组中,品种间的聚类差别较大,表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同;Mantel检测表明,三种标记的分析结果有显著相关性,表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异;非洲菊为组培苗,三种标记的检测结果皆表明,混合样品可以作为个体样品的代表,对整个居群的遗传多样性进行评价;这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

The new plant type Indica and Japonica varieties showed somewhat check heterosis whencrossed with one of the four sterile lines. America Japonica varieties showed few fillers andlower seed set ratio and Africa Japonica varieties, Nuda varieties, Javanica varieties have fewtillers and taller plant height, so they could not be used directly in Japonica hybrid ricebreeding program.

美国稻的分蘖少且结实率较低,产量优势不大;非洲稻的分蘖少,实粒数也少,株高太高,无法直接利用;新株型粳稻与培矮64s、N422s配组有一定的优势,新株型籼稻与N422s、108s配组的优势也较大,在华北两系杂交粳稻育种中也有一定的利用价值;光壳稻和爪哇稻的株高偏高,无法直接利用,可间接利用这两个生态型千粒重大的特点。

After non-inoculated samples were treated with H2O2, peaks of protein had the trend of left-moving in resistant varieties, but trend of right-moving in susceptible varieties. So, when H2O2 existed, enchancement of POD activity impelled low-weight substance to polymerize high-weight protein in resistant varieties, but no difference in susceptible varieties.Samples from callus of five wheat varieties with varying degrees of resistance against wheat head blight were examined by FPLC.

对照样品在过氧化氢水浴(35℃)处理后,抗性品种的蛋白峰有&左移&现象,P_0峰有所提高,表现与赤霉病菌接种处理一致的趋势,因而,在H_2O_2存在的前提下,抗性品种中POD活力的提高促使小分子量物质聚合成大分子量的蛋白,而在感性品种中没有这些变化的出现。

Results indicated that, with cotton varieties succession, in the primary factor of yield constitution, the number of bolls per plant, lint index and lint percentage increased obviously, in which the number of lint percentage in modern varieties was 7.5%~19.9% higher than old varieties, along the lint index increased the seed index obviously drops with cotton variety change; boll weight and the number of bolls per plant displays opposite tendency, there was a declined trend in boll weight, from 5.9g in old varieties to 5.3g in modern varieties.

结果表明:随着棉花品种的更换,在其产量构成的主要因素中,结铃数、衣分、衣指逐步提高,近期品种衣分比以往品种增加7.5%~19.9%;在衣指提高的过程中,子指却在随品种更替明显下降;单铃重表现出与单株结铃数相反的趋势,铃重逐渐减轻,从早期品种的5.9g下降到近期品种的5.3g。

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