查询词典 vaccine-therapy

ChimeriVax has also been applied to development of Sanofi Pasteur's tetravalent dengue vaccine, which has successfully demonstrated proof-of-concept in a Phase 2 trial by generating 100% seroconversion to all four dengue virus serotypes.

ChimeriVax也适用于赛诺菲巴斯德的四价登革热疫苗，已成功地证明，证明在一个第二阶段试验的观念，产生100％转阴到所有四个血清型登革热病毒的发展。

Evaluation of a combined tetravalent diphtheria,tetanus,whole-cell pertussis and hepatitis B candidate vaccine administered to healthy infants according to a three-dose vaccination schedule.

同时用这3项试验检测四联疫苗中全细胞百日咳菌苗、精制白喉类毒素、精制破伤风类毒素的效力，结果均在规程合格范围内。

The study is to improve the producing efficacy of AR vaccine. Toxigenic Pasteurella multocida.(Pm 13-1) was cultured in the 15L BIOSTAT.C fermentor with experimental parameters (Time, Air Flow, Speed of stir, and pH).

为提高猪传染性萎缩性鼻炎疫苗生产效率，采用工作容积15立升的BIOSTAT·C型发酵罐，培养经鉴定作为制苗菌株的产毒素多杀巴氏杆菌Pm 13-1株。

The experiment results showed the characteristics of the fermental Toxigenic Pasteurella multocida could meet the standard of producing AR vaccine in the colony morphology, toxin pathogenesis, and other aspects. The serotype of fermental Toxigenic Pasteurella multocida did not change.

实验结果表明，在适宜条件下D型产毒素Pm13-1株培养物符合其菌落、菌体形态特征；糖发酵实验结果符合其生化特性；其产毒素能力符合对制苗菌株产毒素、致病变能力的要求，且未发生血清型的变化。

Keywords: pancreatic cancer ;vaccine;trimera

中文关键词：胰腺癌；疫苗；三重嵌合

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

Methods A random and open clinical trial was performed in children at more than 18 months of age, who were uninoculated with HA vaccine, and adults at 18~35 years of age in Yancheng City, Jiangsu Province, China.

采用随机、开放式试验设计，选择出生后未接种过甲肝疫苗的18月龄以上的儿童和18~35周岁的成人，接种冻干甲肝减毒活疫苗1针。

To develop novel live attenuated influenza vaccine, we explored the feasibility to attenuate influenza virus by codon deoptimization of NS1. According to the codon usage bias in influenza A virus, we designed and synthesized a condon-deoptimized NS gene by substituting codons of 110 amino acids in the NS1 gene of A/Puerto Rico/8/34(H1N1) with unpreferred synonymous codons.

根据A型流感病毒密码子使用偏嗜性，选取稀有密码子对A/Puerto Rico/8/34(H1N1)病毒NS1基因内部110个氨基酸区域进行密码子同义突变改造，并全基因合成NS基因，利用反向遗传操作技术拯救出含有密码子去优化NS1基因的重组病毒。

Singer, the mother of a child with autism, added,"That study did a lot of harm. People became afraid of vaccinations. This is the Wakefield legacy: this unscientifically grounded fear of vaccinations that result in children dying from vaccine-preventable diseases."

Singer是一个自闭症孩子的母亲，她补充到，&这个研究伤害了很多人，人们惧怕接种疫苗，这都是Wakefield所带来的：这些没有科学依据的对疫苗接种的恐慌，导致许多儿童因那些可预防的疫病而死。&

Weinstein said, There have been several proposed explanations for the rapid spread of HIV in Africa, including wars, the reuse of unsterilized needles and the contamination of early batches of polio vaccine.

温斯坦说：非洲HIV快速传播业已有几种解释：战争、未消毒针头的重复使用以及早期几批脊髓灰质炎疫苗的污染等。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。