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Methods The HBsAg content and RP of key samples at purification step of production of recombinant HB vaccine were determined by Kehu a Plate EIA, Abbott MUREX Plate EIA and ECI, and the results were compared with that by Abbott Bead EIA, based on which the precisions of the three methods were analyzed.

分别用科华板式EIA法、雅培MUREX板式EIA法、罗式电化学发光法与雅培珠式EIA法对比检测重组乙型肝炎疫苗生产过程中纯化工序关键样品的HBsAg含量和疫苗的RP值,并分析3种方法的精密性。

The recombinant baculovirus was transfected into sf-9 cells by CellFectin reagent. SDS-PAGE and Western-blot analysis showed that the expressed protein was 37ku in molecular mass and exhibited specific immunological activity. These results provide foundation for development of a subunit vaccine with the expressed σC protein against Muscovy duck reovirus infection.

在脂质体介导下将rBacmid-σC转染sf-9昆虫细胞,获得重组杆状病毒rBV-σC;SDS-PAGE和Western-blot分析表明,在sf-9细胞中表达了分子质量约37ku的σC蛋白,该蛋白能与原核表达的σC蛋白免疫小鼠血清发生特异性免疫反应,这为以表达的σC蛋白为抗原的亚单位疫苗的研制奠定了基础。

An attenuated vaccine strain of Muscovy Duck Reovirus, which was suitable for propagating in chicken embryo fibroblast, was obtained by serial passage alternately between Muscovy duck embryo fibroblast and CEF.

应用番鸭胚成纤维细胞和鸡胚成纤维细胞交替传代选育出适应CEF繁殖的番鸭呼肠孤病毒弱毒疫苗株。

Methods Using prime-boost immunization strategy,BALB/c mice were inoculated muscularly with HIV-2 gp105 recombinant DNA vaccine and fowlpox virus.

目的:探讨应用prime-boost免疫策略进行HIV-2外膜蛋白重组鸡痘病毒与重组DNA疫苗联合免疫引起小鼠的免疫应答。

Part Ⅱ Objective: To study the immunoprotective and the immunotherapic effects of the new nucleic acid vaccine of the hepatitis B: pVAX1-C3d-S2S; respectively in normal and HBV transgenic mice after being inoculated intra- muscularly with this Eukaryotic Expression Plasmid, such as the in situ production of the expressed protein in muscle tissue section and specific humoral immune response in normal mice、the variation of specific antigen、antibody and HBV DNA, especialy the changes of specific antigen in liver.

第二部分新型乙肝核酸疫苗pVAX1 C3d-S〓S免疫预防效应和治疗作用的研究目的:研究含有新型分子佐剂补体C3d的HBV adw血清型的乙肝核酸疫苗重组真核表达质粒pVAX1-C3d-S〓S接种正常BALB/c小鼠和HBV转基因小鼠后,其免疫预防作用(接种局部肌肉中特异性抗原蛋白的表达和所诱生的体液免疫应答规律)以及免疫治疗作用(血清中特异性抗原的表达、特异性抗体的产生、HBV DNA的消长和肝组织中抗原表达的变化)。

Methods BALB/c mice were inoculated muscularly with HIV-2 gag recombinant DNA vaccine, or recombinant fowlpox virus, or both.

大量制备并纯化HIV-2 gag重组DNA疫苗和重组鸡痘病毒,以肌肉注射的方式免疫BALB/c小鼠,ELISA法检测小鼠血清HIV-2抗体,流式细胞仪测定CD4^+、CD8^+T淋巴细胞亚类数量,乳酸脱氢酶释放法检测脾CTL对HIV-2靶细胞的杀伤活性。

BALB/c mice were co-inoculated muscularly by this plasmid and the nucleic acid vaccine plasmid pVAXGE expressing the HIV-1 (Human immunodeficiency virus type I) gag-gp120 protein. The level of serum antibodies after immunization showed that the specific antibodies against HIV-1 appeared at the second week and raised to the peak level at the sixth week for the co-immunization group.

将它与表达I型人免疫缺陷病毒(Human immunodeficiency virus 1, HIV-1) gag-gp120的核酸疫苗质粒pVAXGE共同肌肉注射BALB/c小鼠,免疫3次后,以ELISA法检测免疫小鼠血清中抗HIV-1抗体水平,结果显示联合免疫组小鼠在免疫2周后已有抗体产生,6周后进入高峰。

In very rare instances, the virus in the vaccine can mutate into a form that can paralyse.

在极少的情况下,疫苗中的病毒可能变异成一种可导致瘫痪的类型。

Currently the main target of Mycobacterium tuberculosis research is to construct new effective anti-tuberculosis vaccine.

研制开发有效的新型抗结核疫苗,是当前结核病研究的主要目标。

There are two types of vaccine: the flu shot and a nasal spray.

有两种类型的流感疫苗:流感针剂和喷鼻剂。

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