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Benzene-acetone (4:1,V/V)was used as developing agent to soparate amidopyrine and barbitone from the compound amidopyrine injection solution on the silica G plate,and Dual wave thin-layer chromatographic densitometry was used to determine amidopyrine (λ_s = 270nm,λ_R=340nm)and barbitone (λ_x=205nm,λ_R=320nm).

以苯-丙酮(4：1，V/V)为展开剂，在薄层硅胶板上将复方氨基比林注射液中的氨基比林和巴比妥分离后，通过双波长扫描测出氨基比林(λ_s=270nm，λ_R=300nm)和巴比妥(λ_s=205nm，λ_R=320nm)的含量

The separation and determination of caproic acid,1-octanol and octyl caproate by high performance liquid chromatography on the Kromasil C18(150 mm×4.60 mm,5 μm) column with V∶V=95∶5 as mobile phase and with refractive index detector were also studied.

按常规有机合成方法合成了己酸正辛酯，采用在Kromasil C18(150 mm×4.60 mm，5 μm)柱上，以V∶V=95∶5为流动相及示差折光检测器的反相高效液相色谱法，30 ℃分离和测定了己酸、1-辛醇及己酸正辛酯。

By the analysis of coal two-step extract by FTIR and comparison of extraction rate, we can see the two-step extraction with combination of cyclohexanone and CS〓-NMP (1: 1, v/v) has higher extraction rate as to coal of different degree of coalification.

4环己酮和CS〓－MP（1：1，v/v）复合溶剂的两步抽提对不同煤化程度的各煤种均保持了较大的抽提率，基本达到对不同煤种的可溶化要求。

SCE for dihydroartemisininoxy benzoic acid hydrazide at Ag electrode BR buffer solution containing 20% ethanol(V/V, pH 7.2) using electroanalytical chemistry method.

在含20%乙醇的BR缓冲溶液（V/V， pH＝7.2）中，利用电分析化学方法测得双氢青蒿素氧基苯甲酰肼(dihydroartemisininoxy benzoic acid hydrazide， DBAH)在银电极上于－0.66 V

The whole analysis was completed on a C〓 column in 18min at a flow rate of 0.5 ml/min, with the mobile phase of methanol-water (93: 7, v: v) containing 75 mmol/L TEOLA and 200 mmol/L HAc, or with the mobile phase of ethanol-water (90: 10, v: v) containing 25 mmol/L TEOLA and 200mmol/L HAc pumped at 0.25ml/min. Compared with the methanol system, the ethanol system was a better candidate for the separation of thulium porphyrin complexes in view of selectivity.

在C〓分析柱（5μm，2.5×150mm）上，流动相为：甲醇：水＝93：7（v/v内含75mmol/L三乙醇胺和200mmol/LHAc），流速为0.5ml/min和流动相为：乙醇：水＝90：10（v/v，内含25mmol/L三乙醇胺和200mmol/L HAc），流速为0.25ml/min，均能使六个铥卟啉配合物在18min内得到有效的分离，相比之下，乙醇体系对铥卟啉配合物更具有分离选择性。

In 2004 Zhang Zhongfu presented the concept ofadjacent vertex distinguishing total coloring whose content is: Let G be a connectedsimple graph with order at least 2, k be a positive integer and f be a mappling fromV∪E to {1,2,...,k}. Let C={f}∪{f|uv∈E,v∈V}for all u∈V. If (1) for any uv, vw∈E,u≠w, we have f≠f;(2) for any uv∈E, we have f≠f, f≠f, f≠f,then f is called a k-proper total coloring of graph G.

第三章和第四章主要研究了两种联图的邻点可区别全染色，邻点可区别全染色的定义是张忠辅在2004年提出来的，其内容为：设G是阶至少为2的简单连通图，k是正整数，f是V∪E到{1，2，…，k}的映射，对任意u∈V，记C={f}∪{f|uv∈E，v∈V}。

Chemiluminescent immunoassay is one of the advanced immunoassay ofnon-radioisotopic immunoassay because of its high sensitivity, wide dynamic range,high accuracy, stable labeled protein, full automation and extensive application field.CLIA using acridinium ester derivatives as chemiluminescent label has advantages oflow background, high signal-to-noise ratio, no need of catalyst and simpleluminescence system. Different aspects associating the DMAE·NHS-based CLIA were studied in thisthesis, including synthesis of acridinium ester, chemiluminescentcharacteristics of DMAE·NHS, labelling antibody or streptavidin with DMAE·NHS,two-site sandwich chemiluminescent immunoassay for TSH, two-sitesandwich chemiluminescent immunoassay involved biotin-streptavidinsystem for TSH, and competitive chemiluminescent immunoassay usingbiotin-streptavidin system for TT〓. The desired acridinium ester, DMAE·NHS, was synthesized according to thereference method with some modifications. The products were identified by IR, NMR,MS and elemental analysis. In our method, KOH was used in place of NaOH tosynthesize benzyl ester of 3,5-dimethyl-4-hydroxybenzoic acid,2',6'-dimethyl-4'-(n-succinimidyloxycarbonyl) phenyl-acridinium-9-carboxylate waspurified on a silica gel column with chloroform/ethylacetate(4:1, v/v) as eluent andfurther purified by triturating with hexane/acetone(2:1, v/v).

DMAE·NHS的合成是本论文的关键和难点，我们对文献方法进行改进：文献方法用氢氧化钠与3，5-二甲基-4-羟基苯甲酸反应制得3，5-二甲基-4-羟基苯甲酸钠，再用3，5-二甲基-4-羟基苯甲酸钠与苄氯作用制备3，5-二甲基-4-羟基苯甲酸苄酯，我们用氢氧化钾代替氢氧化钠，使合成取得成功；在合成2'，6'-二甲基-4'-苯基-吖啶-9-甲酸酯时，文献方法对粗产品进行两次硅胶柱层析纯化，第一次柱层析用氯仿／乙酸乙酯(4∶1，V∶V)作溶剂和淋洗剂，第二次柱层析用己烷／丙酮(2∶1，V∶V)作溶剂和淋洗剂，按照文献方法得到的不是所需要的化合物，我们只进行第一次柱层析纯化，然后用己烷／丙酮(2∶1，V∶V)进行研磨，过滤，洗涤，除去溶于己烷／丙酮(2∶1，V∶V)的部分，得到所需要的产品。

The numerical results of stationary and transient Green'sfunction for high-velocity surface-head wave on some selected crystals are comparedThrough the above analyse,it is indicate that the first arrival wave mode is thelongitudinal-wave head-wave,and its propagate velocity is equal to the groupvelocity of the longitudinal bulk wave alone the surface.Furthermore,this modedoes not have the Δv/v effect,i.e.,its velocity is the same no matter the surface isfree or metalized.

通过对表面激发的瞬态和稳态Green理论分析和数值计算，表明在压电晶体表面上实际上看到的最高速的最先到达的波动模式是纵波头波，它具有沿表面纵波的群速度，而且对压电晶体，它不具有Δv/v效应，即在自由表面和金属化表面其传播速度是一样的，而压电晶体表面波及伪表面波均存在Δv/v效应。

Acceptable resolutions were achieved when it was applied for the separation of dipeptides including Leu-Tyr and Val-Tyr by using 1-butanol-acetic acid-water (4:1:5, V/V/V) solvent system. The proteins including cytochrome C and myoglobin, lysozyme and myoglobin, and fresh chicken egg-white proteins were well separated by 12.5% PEG1000-12.5% K2HPO4-75% water (pH 9.0) and 16% PEG 1000-12.5% K2HPO4-71.5% water (pH 8.0) system.

应用研究表明，采用正丁醇-醋酸-水(4：1：5， V/V/V)和PEG1000-磷酸钾盐-水(12.5：12.5：75， W/W/W)(pH 9.0)体系可以在较高的流动相流速和较高的固定相保留下分别实现对亮氨酸-酪氨酸和缬氨酸-酪氨酸二肽混合物以及细胞色素C与肌红蛋白、肌红蛋白与溶菌酶混合物及鸡蛋清样品中蛋白成分的成功分离。

One structure of using V/v connection and three phase three leg converter under direct or BT supply, one structure of using two single phase converter and V/x connection under AT supply are both researched and simulated detailed.

系统整体结构设计后，以直接供电或BT供电方式下，采用V/v接线变压器和三相三桥臂变流器，及AT供电方式下，采用V/x接线变压器和两个共用一直流环节的单相变流器的系统结构为对象，分别进行详细研究和仿真。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。