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The idiotype TCR Vα1-pIRES-TCR Vβ8 eukaryotic vectors were developed successfully, it would be provided the basic data for the study on specific TCR gene modified T cells.

成功构建了独特型TCR Vα1-pIRES-TCR Vβ8双基因表达载体，为利用特异性TCR基因修饰T细胞的研究提供了基础资料。

Two idiotype TCR Vα1-pIRES-TCR Vβ8 recombinant eukaryotic vectors weredeveloped successfully, and the expression of both idiotypic TCR mRNA and proteincould be detected in transferred A549、K562 and Molt4 cells.

2成功构建了两组TCR Vα1-pIRES-TCR Vβ8表达载体，该表达载体转染A549、K562和Molt4细胞后，可表达相应的mRNA和蛋白。

The rearranged idiotypic CDR3 fragments coding TCR Vβ8 of the Jurkat cell strain and TCR Vβ2 of the Molt4 cell strain were amplified using RT-PCR, and then were cloned into pIRES vector respectively.

用RT-PCR方法扩增特异性重排的Jurkat细胞TCR Vβ8 CDR3序列和Molt4细胞TCR Vβ2 CDR3序列，分别克隆到真核表达载体pIRES中。

The results showed that under 200 W power, in the present of various alkalis, e.g. Na2CO3, NaF, KF, NaHCO3, and DMF-H2O (V∶V=5∶3) used as solvent, PVC-TETA-Pd could catalyze sodium tetraphenylborate to react quantificationally with iodobenzene in 20 min without inert gas protection, and all the phenyl g...

实验发现：在微波功率为200W时，在Na2CO3，NaF，KF，NaHCO3等多种碱的存在下，以DMF的水溶液(V∶V=5∶3)为溶剂，在无惰性气体保护下，PVC-TETA-Pd催化四苯硼钠与碘代苯在20min内即可定量反应，与常规加热方式相比，节省了大量时间；同时也证明了四苯硼钠的四个苯基均可参与Suzuki偶联反应；该催化剂回收再利用3次后，仍具有较高的催化活性。

In order to study the mechanism of V-type CMS wheat, a set of near isogenic lines of restoring gene to wheat V-CMS were used as materials, the mtRNAs of male sterile line, maintainer line and F1 hybrid were compared by cDNA-RAPD method.

为了研究V型细胞质雄性不育小麦的不育机理，以V型细胞质雄性不育小麦的一套近等基因系为材料，利用cDNA-RAPD方法对不育系、保持系和杂种F1线粒体RNA进行了比较分析。

LaNi〓 catalyst was metallurgically combined with V、Nb and TiFe by ball milling, and then V-LaNi〓、Nb-LaNi〓 and TiFe-LaNi〓 nano-compound materials formed.

利用机械球磨方法使LaNi〓催化剂有机地与室温难活化储氢金属V、Nb、TiFe合金结合，形成V-LaNi〓、Nb-LaNi〓、TiFe-LaNi〓纳米复合储氢材料。

The analytes were extracted with 0.2% metaphosphoric acid/methanol (6:4, v/v) then cleaned up by three solid-phase extraction cartridges (Oasis HLB, Oasis MAX and Cl8), and had better results from Oasis HLB cartridges. The recoveries for oleandomycin and erythromycin analysis by developed method were

萃取净化之方法，经测试以0.2%偏磷酸与甲醇(6：4， v/v)之混合液，比较三种固相萃取匣（Oasis HLB， Oasis MAX及C18），结果以利用Oasis HLB固相萃取匣萃取及分析，可得到最佳之回收率及净化效果，且具有良好之再现性。

The HPLC condition consisted of C18 column(250 mm×4.6 mm,5 μm),mobile phase of 0.1 mol/L monopotassium phosphate-methanol(70 : 30,v/v) at the flow rate of 1.0 mL/min and at detection wavelength 280 nm.

本法采用C18色谱柱，流动相为0.1mol/L磷酸二氢钾溶液(用磷酸调节pH值至2.5)-甲醇的混合溶液(按v∶v=70∶30配制)，检测波长为280nm，流速为1.0mL/min。

Degree sum conditions of IM-extendable graphs are researched. The main results are as follows:(1) Let G be a graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,, then G is IM-extendable.(2) Let G be a claw-free graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,d+d≥2n+3, then G is IM-extendable.

研究导出匹配可扩图的度和条件，主要结果如下：(1)若图G有2n个顶点，且对于G中每一对不相邻的顶点u和v，，则G是导出匹配可扩的；(2)若G是一个有2n个顶点的无爪图，且对于G中每一对不相邻的顶点u和v，d+d≥2n+3，则G是导出匹配可扩的。

Degree sum conditions of IM-extendable graphs are researched. The main results are as follows 1 Let G be a graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,, then G is IM-extendable. 2 Let G be a claw-free graph with 2n vertices. If for each pair of nonadjacent vertices u and v in G,du+dv≥2n+3, then G is IM-extendable.

研究导出匹配可扩图的度和条件，主要结果如下 1若图G有2n个顶点，且对于G中每一对不相邻的顶点u和v，，则G是导出匹配可扩的 2若G是一个有2n个顶点的无爪图，且对于G中每一对不相邻的顶点u和v，du+dv≥2n+3，则G是导出匹配可扩的。

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。