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METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

METHODS: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

METHODS摘要: Switch mechanism at 5′end of mRNA template technology, SMART technology, was used to construct the libraries. The purified Poly+RNA was used as template, Powerscript reverse transcriptase was used to transcribe and 5′oligo as a short extended template was added to the 5′ end of mRNA to enrich the fulllength cDNAs.

方法摘要：运用mRNA 5′末端的模板转换方法，即SMART技术，以纯化的Poly+RNA为模板，用Powerscript逆转录酶进行转录，并在mRNA 的5′末端添加一段5′ oligo作为延伸后的模板，从而富集全长 cDNA。

An illustration is normally used once or used for a limited campaign, whereas a logo is used for years and is placed on business cards, letterheads, envelopes, web sites, vehicles, buildings and products.

一个例子是，通常使用一次，或用于有限的运动，而一个标志是使用了多年，并放置在名片，信纸，信封，网站，车辆，建筑物和产品。

An illustration is normally used once or used for a limited campaign, whereas a logo is used for years and is placed on business cards, letterheads, envelopes, web sites, vehicles, buildings and products.

一个例子通常是使用一次或使用有限的运动，而标志是使用多年，并放在名片，信纸，信封，网站，车辆，建筑物和产品。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

The author,as one of the experts who draws the national standards for reciprocating internal combustion engine used in explosive gas environment,present in details about the Ex construction of this kind engine used in this hazardous areas and the Ex technology used in this sort of engin.

通过对爆炸性气体环境用往复式内燃机防爆结构及防爆技术的介绍，提出了往复式内燃机应采取的防爆技术措施及应用在潜在爆炸性气体危险场所的相关安全要求，从而确保内燃机的防爆安全性能。

Chan and D.O. Shah, in which Klearfac AA-270 was used. OPP-n shwwed broadening effects for crude oil/ASP systems. OPP-10 can be conveniently used at high salinity, while OPP-4 used at ...

对克拉玛依原油／ASP复合体系，亦有展宽作用，OPP－10使界面张力向高盐度区单调下降，而OPP－4可使低张力区在较低盐度区展宽。

Chorus oh, you never turned around to see the frowns on the jumpers and the clowns when they did their tricks for you you never understood that it ain't no good you shouldn't let other people get your kicks for you you used to ride on your chrome horse with your diplomat who carried on his shoulder a siamese cat ain't it hard when you discovered that he really wasn't where it's at after he took from you everything he could steal princess on a steeple and all the pretty people they're all drinking, thinking that they've got it made exchanging all precious gifts you better take your diamond ring, you better pawn it babe you used to be so amused at napoleon in rags and the language that he used go to him he calls you, you can't refuse when you ain't got nothing, you got nothing to loose you're invisible now, you've got not secret to conceal Like A Rolling Stone

没了回家的方向、也没人认识像个流浪汉高傲的王子，和所有那些漂亮的人们他们都喝着酒，琢磨着怎么能淘换到更值钱的玩意儿但是你呢，你得把你的钻戒举起来，把它当了人都有倒霉的时候，看着戏里走背字儿的拿破仑你不是曾经还挺得意吗？现在轮到你了，拿破仑在招呼你了，你无路可退了当你一无所有的时候，你不用担心会失去什么了现在你已经透明了，已经没啥可隐瞒的了孤身一人的滋味你觉得怎么样？没了回家的方向、也没人认识像个流浪汉。

In order to disclosure the reason why the electrocatalysis activeness and selectivity droped after being used several hundred hours continuously, the x-ray diffraction, scanning electron microscopy and energy dispersive spectroscope have been used to characterize the surface condition variations of Monel metal. The results indicated: Monel metal belongs to the Cu-Ni alloy around the electrolysis experiment.And after being used for 600hr, the surface layer element composition and geometry distribution of the alloy changed, namely in the alloy the Cu element /which superficial free enthalpy was smaller,"concentrated" to the alloy surface,and formed one kind of marble structure microcrystallite.Thus the electrode surface becamed rough a

为了揭示蒙乃尔合金阴极在连续使用数百个小时后电催化活性剂选择性下降的原因，采用X射线衍射仪、电子扫描电镜和电子能谱分析仪对电解实验所用的蒙乃尔阴极材料在电解实验前后的表面状态进行了表征，结果表明：所使用的蒙乃尔合金阴极材料在电解实验前后均属于Cu-Ni合金，使用600hr后，由于表面层元素组成和几何排布发生了变化，即合金中表面自由焓较小的Cu元素向合金表面&富集&，形成一种大理石结构的微晶，表面粗糙、不均一，这是造成其对硝基苯电还原的催化活性及选择性随着使用时间延长而逐渐下降的主要原因，同时也为此类电极的修饰改进提供了方向。

The network space is the typical form of abstract space when it comes to the digital stage.

网络空间是抽象空间数字化阶段的典型形式。

The Notarial System,as the system of judicial certification in our country,is both the constructor and the defender of social credit system,It has its special value and function in the social honesty.

公证制度作为我国司法证明制度，既是社会信用体系的建设者，同时也是社会信用的捍卫者，在诚信中有其特殊的价值和功能。