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After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响，ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量，检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

The MTT assay was used to detect the viability of cells. Flow cytometry and laser confocal microscopy were used to identiyfy the cell cycle and apoptotic morphology after cells treated with LAO. RESULTS: An L-amino acid oxidase was successfully purified from the venom of bungarus fasciatus.

结果： 通过两步层析法从金环蛇毒中分离得到L-氨基酸氧化酶，L－氨基酸氧化酶的表观分子量通过SDS-PAGE测定约为60 kD，通过凝胶过滤测定分子量为70 kD.L－氨基酸氧化酶作用于HUVCE细胞12 h后，细胞的生长明显受到抑制，L－氨基酸氧化酶作用的半数抑制量为2.8 mg/L。

To investigate feasibility and effect of schizophrenia treatment by stereotactic lesioning, CRW stereotactic apparatus and PICKER spiral CT were used to define nucleus location and RADONICS radiofrequency instruments were used to lesion different subnucleuses of amygdala and the front part of callosal gyrus.

采用CRW立体定向仪和PICKER螺旋CT定位，用RADONICS毁损仪毁损杏仁核不同亚核团和扣带回前部治疗精神分裂症 3 5例，并进行了一年的随访。

To investigate feasibility and effect of schizophrenia treatment by stereotactic lesioning, CRW stereotactic apparatus and PICKER spiral CT were used to define nucleus location and RADONICS radiofrequency instruments were used to lesion different subnucleuses of amygdala and the front part of callosal gyrus.

采用CRW立体定向仪和PICKER螺旋CT定位，用RADONICS毁损仪毁损杏仁核不同亚核团和扣带回前部治疗精神分裂症 3 5例，并进行了一年的随访。以临床疗效总评量表评定疗效标准为指标，评定病人疗效，旨在探讨立体定向毁损杏仁核不同亚核团和扣带回治疗难治性精神分裂症的可行性和疗效。

Methods CRW stereo tactic apparatus and CT were used to define nucleus location and Radonics radiofrequeney instruments were used to lesion different subneucleus of amygdale and the front part of callosal gyrus. All schizophrenic patients were diagnosed by CCMD-3 standards.

方法对32例难治性精神分裂症患者，采用CRW立体定向仪和CT定位，用Radonics毁损仪毁损杏仁核不同亚核团和扣带回前部，采用临床疗效总评量表、简明精神病量表和阳性与阴性症状量表和韦氏智力量表等评定疗效，并进行1年的随访。

Finally, two components mixture of caprylic acid and lauryl alcohol was used to attestate the validity of the process model. The model was also used in the simulation of the paroxetine free base and PB, compared with experimental outcome, analyzed the relatively error between experiments and simulations to further check the rationality of the model, which is beneficial to establish a more perfect model in the future.

应用正辛酸-十二烷醇对模型进行验证，并应用此模型模拟计算了帕罗西汀碱和胡椒基丁醚的分离过程，进一步验证了此模型的合理性，分析了产生实验值与模拟值相对误差的原因，为进一步完善刮膜式分子蒸馏过程模型奠定了基础。

MethodsThe cells of human gastric cancer cell line SGC7901 was treated respectively with Chrysin at different concentration 10、20、40、80μM for 48h. The proliferation inhibitory rate was measured by MTT assay. Acridine orangestaining and flow cytometry were used to detect apoptosis. Western blot assay was used to detect the expression of apoptosis related genes NF拨B,Bcl2,Bax.

方法分别用10、20、40、80μM的ChR处理人胃癌细胞株SGC7901 48h，采用MTT比色法检测ChR对SGC7901细胞的增殖抑制效应；采用丫啶橙染色、流式细胞术检测ChR诱导SGC7901细胞凋亡的发生；应用Westernblot法检测凋亡相关基因NF拨B、Bcl2、Bax的蛋白表达，并用计算机图像分析软件进行半定量分析。

The main conclusions are as follows:The vitellin of Chrysopa septempunctata was extracted with 0.4mol/L NaCl and then precipitated in cold distilled water. Ion exchange chromatography and proteic gel recovery were used to purify it further. SDS-PAGE analysis shows that the vitellin is composed of two subunits, the large one 165kDa and the small one 46kDa.Then automatic amino acid analyzer was used to analyse amino acid composition of vitellin.

主要结论如下：用蒸馏水沉淀法对大草蛉的卵黄蛋白进行了初步纯化，而后又采用离子交换层析和蛋白胶回收的方法对卵黄蛋白进行了进一步的纯化；通过SDS-PAGE分析表明，大草蛉的卵黄蛋白由大165kDa和小46kDa两个亚基组成；用氨基酸自动分析仪对纯化后的卵黄蛋白进行了氨基酸组分分析。

METHODS:(1) Ethanol extraction and ethyl acetate purification were used to extract isofiavones from Cicer arietium L.. Orthogonal design was used to optimize the better Ethanol extraction conditions.

(1)以新疆地产鹰嘴豆为原料，经脱脂，乙醇提取，乙酸乙酯萃取，得到总异黄酮，采用正交试验优选出最佳工艺。

For instance, a colonoscope is used to detect growths inside the colon, and a laparoscope is used to examine the abdominal cavity.

例如，结肠镜被用于检查结肠，腹腔镜被用于检查腹腔。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。