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A glass die, a micro replication characteristic of PDMS and an electroplating technology are used to make the three-dimensional microelectrode array. A precise cutting machine is used to cut the glass to form a column array and a mixture of HF and NH4F is used to produce the corresponding structure serving as the die of the micro replication of PDMS. The photoetching technology is left out and the limitation of a photoresist on the dimension of the three-dimensional structure is eliminated; therefore the longer microelectrode array can be made. The length of the microelectrode array depends on the incision depth of the glass and the electroplating technology of deep hole of the metal.

本发明利用玻璃模具、PDMS的微复制特性和电镀技术制作三维微电极阵列，用精密切割机切割玻璃形成柱状阵列，用HF和NH 4 F的混合液腐蚀出相应的结构作为PDMS微复制的模具，免去了光刻工艺，消除了光刻胶对三维结构尺寸的限制，可以制作更大长度的微电极阵列，这一长度决定于玻璃的切割深度和金属的深孔电镀技术。

Sims and Saleh performed pin site care daily beginning 48 hours after the operation. In their study, they applied Orthox monolateral xators (Orthox Srl, Bussolengo–Verona, Italy)(58.4%), circular xators (19.7%), and the Shefeld Hybrid System (21.9%). Massage was used to release skin adhered to the pin. A cotton swab with normal saline or cooled boiled water was used to clean the pin site. Another cotton swab was then used to dry the pin site. Dressings were only applied if there was any exudate. Bathing with the leg submerged in water was discouraged. They found that 29% of the xators had noinfection (grade 0) according to the grading system of Saleh and Scott (Table 5). The remaining 71% of xators had an infection of one or more different grades. There were no grade 6 infections. They concluded that xators located on the femurhad the highest infection rate and that incidence of infection was 2.5 times greater for femoral xators in which corrections were performed (62.3%) than in those without corrections (24.5%). These observations, however, were based on half-pin care. We did not use half-pins or Schanz screws in any of our treatment groups.

Sims and Saleh在手术后48小时开始进行没人针孔护理，在他们的研究中，他们应用Orthox单臂外固定架(Orthox Srl， Bussolengo–Verona， Italy)（58.4%），环形外固定架（19.7%），Shefeld组合式外固定架（21.9%），按摩放松附着于钢针上的皮肤，蘸有生理盐水或凉开水的棉拭子用于清理针孔，另外一个棉拭子用于蘸干针孔，只有局部出现渗出是才应用敷料，阻止患者进行将带有外固定架的肢体浸入水中的洗浴方式，他们按照Saleh and Scott的标准发现29%的外固定架没有感染（0级），剩余71%外固定架有1个或者多个级别的感染，他们没有6级感染，他们得出结论：位于股骨的外固定架有最高的感染几率，而且实施矫形患者的感染发生率（62.3%）是不进行矫形患者感染发生率（24.5%）的2.5倍，但是，这些观察是基于半钉护理，在我们的任何一个治疗组中都没有应用半钉和Schanz螺钉。

The main jobs of this paper are as followed: 1 The reuse of the frame design technology for aircraft assembly fixtures is researched. a frame line part library is built with the templet driven technology, the reuse of the frame line part come true by the tools for instantiation and instance modification. The design resources are effectively used, some repeating work is cut. 2 Two methods to obtain the assembly feature are proposed: the straight way by the Enumeration method and the indirect way by project features to the assembly plane. All of the assembly features of the frame and the adjustable bearings are obtained by these two methods which makes the frame automatic assembly technology for aircraft assembly fixtures come true. 3 The frame automatic assembly technology aircraft assembly fixtures based on assembly features is researched. A binary tree assembly model is used to express the assembly information of the frame for aircraft assembly fixtures, the automatic assembly between the frame, the aircraft and the adjustable bearing is completed within the three steps: distribute, obtain and evaluate assembly feature. Finally, the efficiency of the frame assembly design is proved. 4The frame interpart modeling technology for aircraft assembly fixtures is researched, the intersection methods are concluded and the arithmetic of the single-cut, double-cut and inter-cut intersection is proposed, the interpart fast modeling of the frame design for aircraft assembly fixture come true. 5Based on the researches mentioned before, a sub-system of frame fast design for aircraft assembly fixture is developed on CATIA. The sub-system is used to design the aircraft assembly fixtures in a large aeronautics corporation and make a good- effect.

论文的主要工作如下： 1研究了飞机装配型架骨架设计重用技术，在总结归纳骨架线框结构类型的基础上，应用模板驱动的方法建立了骨架线框库，通过骨架线框零件的实例化和离线编辑实现骨架线框的重用，有效利用设计资源，减少重复劳动； 2提出穷举几何对象的装配特征直接获取方法，遍历零件模型的几何对象，从中捕捉所需装配特征，该方法简捷、高效；提出向装配面投影的装配特征间接获取方法，该方法在现有特征基础上生成装配特征，解决了部分装配特征难以直接获取的问题； 3研究了基于装配特征的飞机装配型架骨架自动装配技术，采用一种二叉树装配模型表达骨架的装配关系，并通过装配特征的分配、获取和赋值三个步骤，实现了骨架分别与飞机产品和可调支承的自动装配，避免了繁琐的人工交互操作，提高了骨架设计的效率和规范化程度； 4研究了飞机装配型架骨架零件间建模技术，在总结归纳骨架元件相贯处理方式的基础上，提出了骨架元件单剪切相贯、双剪切相贯和互剪切相贯的实现算法，简化了骨架相贯处理的过程； 5基于以上研究，在CATIA平台上开发了飞机装配型架骨架快速设计子系统，并应用于某大型航空企业的装配型架设计，提高了设计效率和设计质量。

The frequency range and distortion model affected by Faraday rotation were analyzed. A kind of calibration algorithm was introduced, in which trihedral corner reflectors were used to calibrate POLSAR image subject to channel imbalance, Faraday rotation and RCS distortion. [2]Three kinds of target decomposition theorems used to extract polarimetric features of SAR image were studied in detail. A kind of POLSAR MLC image data decomposition method based on classical coherent decomposition theorem of POLSAR SLC image was introduced, which was used to extract polarimetric features of POLSAR MLC image.

当工作频率小于1GHz时，法拉第旋转是影响低频星载极化SAR图像定标的一个重要因素，论文分析了受法拉第旋转影响的波段范围和误差模型，提出了一种校正通道不平衡度、法拉第旋转和绝对幅度增益的极化定标算法，仅使用一种点目标——三角反射器作为定标体，应用于受法拉第旋转影响的极化SAR图像定标； [2]深入研究了三类用于SAR图像极化特征提取的目标分解理论。

Methods Density gradient centrifugalization combined with adherence method were used to segregate and cultivate rat MSCs. MSCs cultivated to the 3 rd passage were characterized using flow cytometry technique. TGF-β1 with 1, 2 and 5 μg/L were pretreated on MSCs for 8 h, then serum and oxygen deprivation were used to cause analogous ischemia of MSCs in vitro. AnnexinV/PI flow cytometry technique was used to evaluate apoptosis and survival of MSCs.

采用密度梯度离心结合贴壁法分离、培养大鼠MSCs，对培养第3代的MSCs进行鉴定；用1、2、5μg/L的TGF-β1分别预处理细胞8 h，然后用血清剥夺和低氧处理建立MSCs体外模拟缺血性损伤模型，通过流式细胞术评价MSCs的凋亡及存活情况；用Western blot方法检测2μg/L的TGF-β1对Akt和p-Akt表达的影响。

Inspected gathered in advance the body preparation method influence, the neutralizaing agent triethylamine to the disperser craft and the stability of emulsion influence, the water affinity chain extendor DMPA amount used to the river character polyurethane emulsion performance influence, the isocyanogen diethylene glycol dinitrate amount used to the river character polyurethane emulsion performance influence and the soft section low-molecular polymer dihydroxy alcohol amount used to the river character polyurethane emulsion performance influence.

考察了预聚体制备方法的影响，中和剂三乙胺对分散工艺及乳液稳定性的影响，亲水扩链剂DMPA的用量对水性聚氨酯乳液性能的影响，异氰酸酯的用量对水性聚氨酯乳液性能的影响及软段低聚物二元醇的用量对水性聚氨酯乳液性能的影响。

The results indicated that both binary interactive parameters of water/isobutanol system from this work and the parameters of water/ethanol from literature can be used to precdict the liquid-liquid equilibria of water/ethanol/isobutanol. But the parameters of ethanol/isobutanol from the literature were not appropriate for the prediction. Furthermore, the model parameters from 30℃ can can be used to predict the liquid-liquid equilibria under 35 ℃. The model parameters of water/isobutanol are independent on the temperatures, so constant parameters irrelative to temperature can meet prediction accuracy. Othmer-Tobias/Bancroft equation was also used to fit the liquid-liquid data of water/ethanol/isobutanol system, but it didnt possess the performance of predicting the liquid-liquid equilibria.The partition coefficients of dipotassium glycyrrhizinat were determined in the liquid-liquid two-phase system of water/ethanol/isobutanol and corrected by experiential equation.

结果表明：由本文的二元液液相平衡实验数据关联得到的水+异丁醇体系的相互作用参数和文献上由二元气液相平衡数据关联得到的水+乙醇体系的相互作用参数都具有预测三元液液相平衡关系的能力，而文献上由二元气液相平衡数据关联得到的乙醇+异丁醇体系的相互作用参数不具有预测三元液液相平衡关系的能力；可以用从30℃三元液液相平衡数据关联回归得到的模型参数来预测35℃时的液液相平衡关系；水+异丁醇的相互作用参数与温度关系不是很密切，计算时取与温度无关的常数即能满足关联精度要求；Othmer-Tobias和Bancroft经验方程可用于水+乙醇+异丁醇两相体系的液液相平衡关系的关联计算，但这类经验方法并不具有预测能力。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Methods Emergency treatment should be quickly, 0.1%adrenalin hydrochloride gauze was used to oppress the lacerative position, aseptic gauze was used to tamp in order to further compression hemostasis, or used catgut to suture the lacerated vagina. For the blood loss was more, we established the venous transfusion quickly, observed vital signs and vaginal bleeding situation, kept perineum cleanly and dry, prevented infection, strengthened psychological nursing and health guidance.

应急处理应及时迅速，采用将浸有0.1%盐酸肾上腺素纱布压迫裂伤部位，填塞无菌纱条进一步压迫止血，或采用肠线缝合裂伤的阴道，出血量较多者，迅速建立静脉通道，监测生命体征及阴道流血情况，保持会阴清洁、干燥，防止感染，加强心理护理和健康指导。

On the license location,the projection was used to locate the license plate; On the characters segmentation, the liminal rule was used to divide the characters; In order to solve the problem of the digital characters recognition in the plate, BP nerve network was used to recognize the digital characters.

本文对车牌识别系统中的车牌定位、字符分割和字符识别进行了初步研究。对车牌定位，本文采用投影法对车牌进行定位；在字符分割方面，本文使用阈值规则进行字符分割；针对车牌图像中数字字符识别的问题，本文采用了基于BP神经网络的识别方法。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了