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The present invention relates to a process for rapid solution synthesis of a peptide, the process comprising repetitive cycles of steps-: a coupling step, using an excess of an activated carboxylic component to acylate an amino component, a quenching step in which a scavenger is used to remove residual activated carboxylic functions, wherein the scavenger may also be used for deprotection of the growing peptide, one or more aqueous extractions and optionally, a separate deprotection step, followed by one or more aqueous extractions, characterised in that the process comprises at least one step, referred to as step, in which an amine or a thiol comprising a free anion or a latent anion is used as a scavenger of residual activated carboxylic functions.

本发明涉及快速溶液合成肽的方法，该方法包括步骤-的重复循环：偶联步骤，使用过量的活化羧基成分酰化氨基成分，淬灭步骤，在该步骤中使用清除剂除去残余的活化羧基官能团，其中该清除剂还可以用于使生长中的肽去保护，一或多个水抽提和任选地，独立的去保护步骤，之后进行一或多次水抽提，其特征在于该方法包括至少一个步骤，称作步骤，在该步骤中包含自由阴离子或潜阴离子的胺或硫醇用作残余活化羧基官能团的清除剂。

The technique of boundary-fitted coordinate system is used to overcome the difficulties resulting from the complicated shapes of natural river boundaries;the method of alternating direction implicit finite difference scheme is used to solve the partial differential equations in the transformed plane;and the technique of moving boundary is used to deal with the river bed exposed to water surface.

用适体坐标技术克服天然河道复杂的几何边界给有限差分法所带来的困难；用交替方向隐式有限差分格式求解变换平面上的偏微分方程；用动边界技术处理河床的淹露。

I used to imagine that if I am a famous singer. I will choose to live in banlieue. I used to imagine that if I am an artist. I will publicize myself very well. I even used to imagine that if I am a dog. I will be the cutiest one.

我曾想过如果我是一名非常出名的歌手，我会选择居住在郊区；我曾想过如果我是一名艺术家，我应该会很好的宣传我自己；我更曾想过如果我是一只狗，我将会是最可爱的一只。

Because different cell lines may show various levels of susceptibility to adenovirus infection and virus production, non-selectively replicating wtAd5 was used to be a comparison with the tested conditionally replicating adenovirus CNHK500.ONYX-015, the first replication-competent adenovirus entered into clinical trials was also used to be a comparison. We used virus proliferation assay, cell viability assay and western blot to evaluate the proliferation and cytolysis selectivity of CNHK500. Results demonstrated that the CNHK500 virus proliferation multiples in breast cancer cell lines after 48 hours is similar to that of wtAd5, ONYX-015 virus proliferation ability is less than that of CNHK500 in cancer cells.

然后，通过与ONYX-015（E1B 55 KD a蛋白缺失的2型和5型嵌和型腺病毒，美国ONYX生化制药公司研制）、wtAd5进行对比，利用病毒增殖实验和细胞生长抑制实验，验证了CNHK500选择性增殖能力和肿瘤特异性杀伤作用；利用Western Blot检测CNHK500腺病毒E1A和E1B在乳腺癌细胞和正常成纤维细胞中的表达差异，揭示了腺病毒肿瘤靶向性的机制；利用携带绿色荧光蛋白的CNHK500-EGFP感染乳腺癌细胞株和正常成纤维细胞株，直观地观察其感染乳腺癌细胞的能力和在乳腺癌细胞中的增殖过程。

Never Say Goodbye Bon Jovi Crossroad As I sit in this smokey room The night about to end I pass my time with strangers But this bottle's my only friend Remember when we used to park On Butler Street out in the dark Remember when we lost the keys And you lost more than that in my backseat baby Remember how we used to talk About busting out - we'd break their hearts Together - forever Never say goodbye, never say goodbye You and me and my old friends Hoping it would never end Never say goodbye, never say goodbye Holdin' on - we got to try Holdin' on to never say goodbye Remember days of skipping school Racing cars and being cool With a six pack and the radio We didn't need no place to go Remember at the prom that night You and me we had a fight But the band they played our favorite song And I held you in my arms so strong We danced so close We danced so slow And I swore I'd never let you go Together - forever Never say goodbye, never say goodbye You and me and my old friends Hoping it would never end Never say goodbye, never say goodbye Holdin' on - we got to try Holdin' on to never say goodbye I guess you'd say we used to talk About busting out We'd break their hearts Together - forever THE END by lune

不要说再见邦乔维十字路口正如我坐在这个熊室夜晚即将结束传递我的时间与陌生人但是，这瓶是我唯一的朋友请记住，当我们用公园对巴特勒街在黑暗请记住，当我们失去了钥匙和你失去了，在我的后座婴儿还记得我们用来谈论关于破坏了-我们要打破他们的心在一起-永远从来没有说再见，从来没有说再见你和我和我的老朋友希望它会永远不会结束从来没有说再见，从来没有说再见握住'的-我们得尝试握住就永远不会说再见记住天逃学赛车酷六包和电台我们不需要没有地方可去记得那天晚上的舞会你和我，我们进行了斗争但是，带他们打我们最喜爱的歌曲举行你和我在我的武器如此强大我们跳舞如此接近我们跳舞如此缓慢，我发誓我从未让你去在一起-永远从来没有说再见，从来没有说再见你和我和我的老朋友希望它会永远不会结束从来没有说再见，从来没有说再见握住的-我们得尝试握住就永远不会说再见我猜你会说我们用来谈论关于破坏了我们想打破他们的心在一起-永远再见 LUNE

Whenpyridine was used as reductive decomposing agent, the expecting cholest-4-en-3,6-dione-22-al was obtained. However, when Zn/HOAc was used to decomposethe ozonide, C-4,5 double bond was further reduced to give cholest-3,6-dione-22-al as main product. 2 For the side-chain construction of compound (1), the solid-liquid phase-transfer Wittig reaction was used. When (3-methyl-2-oxobutyl)-triphenylarsonium bromide was used as reagent, the reaction can be carried out inmild reaction condition and the yield is higher than that when phosphorane wasused.

但使用Zn／HOAc作为分解剂时，却得到其氢化产物胆甾-3， 6-二酮-22-醛；2在甾体支链引入过程中，采用固-液相转移Wittig反应的方法，用(3-甲基)-2-氧丁基三苯基溴化胂作为试剂，反应在非常温和的条件下便可完成，而且产率比传统采用膦烷的方法更高；3在对某些甾酮化合物的选择性还原反应研究时，发现在〓还原体系中，当加入某些金属离子后，反应有更好的区域选择性和立体选择性。

The questionnaire method and the interview menthod were used to gain the tourist data, the quota sampling method was used in sampling survey, the principal component analysis method was used to measure the various variables, the statistical methods such as linear regression analysis, multivariate linear regression analysis, single factor variance analysis and logistic regression analysis were used to analyzes the relationship between various variables.

具体来说，在获取游客数据时采用了问卷调查法和访谈法，在抽样调查时采用了配额抽样方法；对各变量进行测度时使用了主成分分析法；在分析各变量之间相互关系时采用一元线性回归分析、多元线性回归分析、单因素方差分析及Logistic回归分析等统计方法。

Remove row and column' and 'put a big number' have been used for the first boundary;a continuous Regional Identification Function is used on the basis of non-variant nodal virtual flux method for the free surface boundary;Gauss formula is used to make the curved surface integral of the free surface boundary transformed as the difference between volumn integral and other curved surface integral to calculate the integral item on boundaries, which avoiding finding the position of free surface, while the continuous Regional Identification Function is applied to calculate volumn integral and surface integral;Crout and PCG method is used for solusion.

对于已知水头边界，采用&去行去列法&和&置大数法&进行处理；对于自由面穿过的单元，在固定网格节点虚流量法的基础上，引入连续的区域识别函数；对于非稳定渗流中自由面边界积分项，采用高斯公式将求自由面的面积分转化为求体积分与其他面积分之差，避免了求自由面的具体位置，同时在计算体积分和面积分时采用连续的区域识别函数；在解法上，采用直接解法和PCG法。

In this model, the skeletal storage coefficient was used for coupling, different storage coefficient were used for the calculation of storage change of aquifer and interbeds, the elastic and inelastic skeletal coefficients were used to account for the elastic and inelastic deformation, a one-dimensional diffusion equation was used for describing the delayed dissipation of unequilibrated heads within the interbeds, these make the model not only be able to simulate the elastic and inelastic deformation of soil, but also the delay phenomenon.

在该模型中，用夹层的骨架弹性储水系数进行地下水流模型和土体变形模型的耦合，用不同的弹性储水系数来计算夹层和含水层内的储水量变化，用夹层的骨架弹性储水系数和非弹性储水系数来体现夹层的弹性和非弹性变形，用扩散方程来描述滞后夹层内的水头分布，从而使模型能够很好地体现夹层的弹性和非弹性压缩，并能同时考虑夹层变形的滞后现象。

This is most commonly used as a basic form of English to be taught for beginners in various parts of Asia. Special English is commonly used by the voice of America with a maximum of only 1500 words. English reform modifies the English language. Sea speak is developed on a basic set of restricted vocabularies. The most widely used form of English as a second language in Europe is known as the Euro-English. Manually coded English is generally used to educate the deaf people.

在亚洲的许多地区，基础英语是英语初学者们的基本学习内容；特殊英语通常为&美国之音&所用，其词汇量不超过1500；英语改革重塑了英语；专业英语是基于某一特定领域内的词汇发展起来的；在欧洲，被作为第二语言的欧式英语是最使用率最高的英语类型；手势英语通常用于对耳聋人士的教育。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。