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One, study character is in us in education, discover not hard, the student's study, dependence is very strong, always be used to a teacher all-around specific arrangement, once give them time, a lot of students appear not know what to do, faineant even; is studying a method, be used to machinery repeating, face the study rhythm during study and capacity, times feeling gets tired of the study mood; that dealt with; him student to affect them with the expectation fixed position of parent impropriety many students and parent often the study result with the static state, regard only hope as orientaton, not to be pooh-poohed makes dynamic analysis at whole to learning process, undertake ego is adjusted scientificly....

一、学习品质在我们在教学中，不难发现，学生的学习，依靠性很强，总习惯于教师全方位具体布置，一旦把时间交给他们，很多学生就显得无所适从，甚至无所事事；在学习方法上，习惯于机械重复，面对学习期间的学习节奏和容量，倍感疲于应付；学生本人和家长不适当的期望定位影响了他们的学习情绪；不少学生和家长往往以静态的学习成绩，作为唯一的期望取向，不善于对学习全过程作出动态分析，科学地进行自我调适……。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Methods Granulocyte/macrophage colony stimulating factors and interleukin 4 were used to cultivate DC from peripheral blood of hepatocellular carcinoma patients.Tumor associated antigen extracted from human liver cancer HepG2 cell line was used to activate the DC,which will initiate homogenic T lymphocyte to form cytotoxic T lymphocyte.HepG2 cells were transplanted to the nude mice and were treated with CTL.Expression of PCNA in tumor cells was evaluated.

联合应用粒/巨细胞集落刺激因子及白介素-4(IL-4)直接从肝癌患者外周血中培养出DC；以源于人肝癌细胞系HepG2细胞的肿瘤抗原粗提物刺激DC；DC激活同源的T淋巴细胞产生细胞毒性T淋巴细胞；建立人肝癌细胞系HepG2裸鼠移植瘤模型；CTL治疗人肝癌细胞系HepG2裸鼠移植瘤；检测移植瘤标本肿瘤细胞PCNA表达水平。

In the pretreatment group, Bak Foong Pill extracts were given for 14 days prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后，用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化；同时应用免疫组织化学法检测Bax蛋白表达；应用高效液相-电化学方法检测纹状体巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

In the pretreatment group, Bak Foong Pill extracts were given for 14 daya prior to MPTP. Immunostaining of tyrosine hydroxythase was used to observe the damage of dopamine neurons, and of Bax to detect the protein levels of Bax. High performance liquid chromatography-electrochemical detection was used to investigate the contents of DA and its metabolites dihydroxy-phenylacetic acid and homovanillic in the Str.

给予BFP预处理后，用酪氨酸羟化酶免疫组织化学法观察黑质神经元的变化；同时应用免疫组织化学法检测Bax蛋白表达；应用高效液相－电化学方法检测纹状体多巴胺及其代谢产物二羟基苯乙酸和高香草酸的含量变化。

In this thesis, firstly, some main models have been checked on their accuracy, and the reliability of MACORS code has been verified by the RELAP5/MOD2 code on five transient conditions for QSNP: loss of flow; loss of offsite power; incontrollable control rod withdrawal; the feed-water temperature increasing and the feed-water lost. The results obtained are very satisfactory. Secondly, MACORS is used to calculate the operating and accident transients for QSNP, and the analysis results are significant to real engineering. And finally for MNRS, MACORS is used to simulate the steady-state natural circulation heat-removed capacity and the transient behaviors from forced circulation to natural circulation. Some important conclusions are obtained by the analysis results.

本论文首先从各方面对核动力系统中最主要的、热工水力特性最复杂的反应堆和蒸汽发生器进行单独验证和理论分析，其次分别利用RELAP5/MOD2程序和MACORS程序对秦山核电站的双环路失流、全厂断电、反应性事故引入、主给水参数变化及丧失主给水五种瞬态工况进行了计算，结果符合很好，从而证明了本程序模型的准确合理性，选择数值方法的有效性和编制程序的正确性；尔后，利用MACORS程序对秦山核电站的多种运行及事故瞬态进行了分析，得出了具有工程价值的结论；对船用核动力装置的稳态自然循环载热能力以及相应参数的特性、强迫循环与自然循环的过渡工况等进行了仿真分析，所得结论对我国核动力船舶的设计，运行以及安全审评具有重要的意义。

In this way, the credibility of evidence is very important, so the second research question is how the credibility of evidence is achieved by using these main appraisal resources, that is, how the authenticity, relevance and legality of evidence are better proved through the employment of these main appraisal resources.with regard to the third research question, jef verschueren's adaptation theory, especially the three worlds, that is, physical world, mental world and social world, is used to explain why these main appraisal resources are used to better achieve the goals of courtroom participants.it is hoped that this thesis, according to the analysis of ten civil courtroom trial discourses, is going to shed some light on the study of courtroom trials from the perspective of linguistics, enrich the scope of application of appraisal theory and enlighten some scholars in the legal field.

由此可见，证据的可信度在民事法庭审判中是十分重要的，因此，本文的第二个研究问题就是法庭审判的各方是如何通过主要评价资源的应用来更好的说明己方证据的可信度以及对方证据的不可信的，即庭审中的各方是怎样通过使用主要的评价资源来达到证明己方证据的可信度的。对于第三个研究问题，该论文使用jef verschueren's的顺应理论，特别是三个世界(物理世界、心理世界以及社交世界)的理论来解释为什么要使用这些主要的评价资源。该论文的意义在于通过对十个民事法庭审判语篇的研究来给从语言学的角度研究法庭审判提供一定的思考，扩大评价理论应用的范围以及给法律界的学者们一些启发。

Bang Up-to-date and Easy to Use- References to over 170,000 words, phrases and examples explained in clear and natural English- All the important new words that have come into the language (e.g. dirty bomb, lairy, 9/11, clickable)- Over 200 Common Learner Error notes, based on the Cambridge Learner Corpus from Cambridge ESOL exams- Only words from a defining vocabulary used to help make definitions easy to understand- Over 90,000 examples, mainly full sentences, show you how English is used in context and help you write natural English.

邦截至到最新的和易于使用，引用超过17万单词，短语和例子解释清楚，自然的英语所有重要的新话已到语言，例如肮脏炸弹，lairy，9 / 11，可点击-超过200共同学习笔记错误的基础上，剑桥学习者语料库从剑桥ESOL考试，只有一个词从定义词汇用来帮助作出定义很容易理解，在90，000例子，主要是完整的句子，告诉你如何使用英语语境，并帮助您写英文。

Methods: 5% propranolol emulsion was applied externally on the back of a guinea pig\' s ear to produce the psoriasis-like dermatitis model to observe the effects of Baibi ointment on the histopathological changes of the model.Radioimmunoassay was used to observe the levels of cytokines like IL-2、IL—6 and IL-8, and the effects of the ointment to the cytokines. MTT method was used to study the effects of the extracts of the Baibi Formula and the extracts of its main components Huangjing , Kushen , Dahuang ,Huangbai, to the multiplication of HaCaT cells—one of the Malpighian cell line with and without stimulation of TNF-α.

实验采用5%心得安乳剂建立豚鼠银屑病样皮炎模型，观察白疙软膏对豚鼠银屑病样皮炎模型组织病理改变的影响；采用放射免疫法检测豚鼠银屑病样皮炎模型皮损中细胞因子IL-2、IL-6、IL-8的水平，观察白疕软膏对豚鼠银屑病样皮炎模型皮损中细胞因子的影响；采用MTT法研究了中药白疕方提取液及白疕方主要组成药物大黄、黄柏、黄精、苦参提取液对角质形成细胞系HaCaT细胞增殖的影响及对TNF-α刺激后的角质形成细胞系HaCaT细胞增殖的影响。

I am twenty-two blocks from where Rudolph Valentino lay in state, eight blocks from where Nathan Hale was executed, five blocks from the publisher's office where Ernest Hemingway hit Max Eastman on the nose, four miles from where Walt Whitman sat sweating out editorials for the Brooklyn Eagle, thirty-four blocks from the street Willa Cather lived in when she came to New York to write books about Nebraska, one block from where Marceline used to clown on the boards of the Hippodrome, thirty-six blocks from the spot where the historian Joe Gould kicked a radio to pieces in full view of the public, thirteen blocks from where Harry Thaw shot Stanford White, five blocks from where I used to usher at the Metropolitan Opera and only 112 blocks from the spot where Clarence Day the elder was washed of his sins in the Church of the Epiphany (I could continue this list indefinitely); and for that matter I am probably occupying the very room that any number of exalted and somewise memorable characters sat in, some of them on hot, breathless afternoons, lonely and private and full of their own sense of emanations from without.

就这类轶事拉一张单子可以长得没完没了。）依同理，我此刻置身其中的客房可能不知被多少显贵和在某一方面值得缅怀的人物占用过，其中某些人在炎热又闷塞的下午，同样感到落寞而离群，又满怀各人对于从户外传来的人事影响的敏感。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。