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The resultsindicatedthat :combination of urea and so-da had very high effect of increasingthe crude protein content in straw,combination of over 5 percent quickli me and urea hadthe highest efficiency,4 ti mes of that of the ammoniation method with5 percent urea...

结果表明:尿素与氢氧化钠的组合对提高秸秆粗蛋白含量极为有效,5%以上的生石灰与尿素组合提高效率最高,为单纯5%尿素氨化法的4倍。建议生产中根据需要确定尿素剂量。

In Chapter 6, an extensive kinetic study of the inhibition of ribonuclease A and papain by urea has been performed. The results suggest that the effect of urea on activities of these enzymes can be well described by the denaturant binding model. The binding constants of urea determined by the present method are nearly identical to that determined from a variety of different studies on model compounds and proteins.

我们在第六章中研究了脲作用下核糖核酸酶A和木瓜蛋白酶的抑制动力学,发现变性剂结合模型可以很好地描述脲对这两个酶活力的影响,而且用我们自己建立的方法测得的脲与蛋白质的结合常数与前人通过对模型化合物和某些蛋白质的研究所获得的数值基本一致。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数,结果表明:①酶-底物二元、三元复合物的失活速度明显慢于游离酶,说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用;②在脲作用下,酶的失活和构象变化均为单指数项过程,而且酶的活力丧失和三级结构变化是同时发生的,说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程;③在盐酸胍作用下,酶的构象变化为两相过程,而失活则是单指数项过程,酶分子构象变化的快相速度常数与失活速度常数基本一致,因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

The biological activity tests show that some target compounds have a good activity as plant growth regulator, and N-5-(3-carboxy-1H-1,2,4-triazolyl)-N'-o- chlorophenylfonnyl urea, N-5-(3-carboxy-1H-1,2,4-triazolyl)-N'-o- bromophenylformyl urea, N-5-(3-carboxy-1H-1,2,4-triazolyl)-N'-p- methyl-phenyl formyl urea have excellent activity of auxin.

生测试验证明部分酰基脲类化合物具有良好的植物生长调节活性,其中N-5(3-羧基-1,2,4-三唑基)-N'-o-氯苯甲酰基脲、N-5-(3-羧基-1,2,4-三唑基)-N'-o-溴苯甲酰基脲和N-5-3-羧基-1, 2,4-三唑基)-N'-p(或m-甲基苯甲酰基脲具有优良的生长素活性。

Objective: 1)Observe the feasibility of the model of egg protein induced leporid rheumatoid arthritis. 2)By pathology observation of pathological synovium, find out the transformation of AIA leporid arthral synovium after urea injection and the infection to local and general disease. 3)Find out if urea has obvious side-effect to arthral cartilage. 4)Mensurate the VEGF expression of synovium, investigate the effect of VEGF on rheumatoid arthritis and the effect of urea on VEGF expression.

研究目的:(1)观察鸡卵蛋白诱导性兔类风湿性关节炎模型实验方法的可行性;(2)通过病变滑膜组织病理学观察,了解注射尿素后AIA兔关节滑膜病理形态的变化;以及对局部及全身病情的影响;(3)观察尿素对关节软骨是否有明显毒副作用;(4)测定滑膜VEGF表达,初步探讨VEGF在RA病变发展中的作用及注射尿素对VEGF表达的影响。

Urea inclusion method was adopted with a stepped temperature dropping to purify ω-3PUFA. The optimum ratio of ester-urea is 1 : 15. After first separation cycle, the content of ALA in perilla oil and the total content of both EPA and DHA in fish oil were increased to 85.68% and 67.32% respectively. With the increase of the urea amount, the contents of ALA and EPA+DHA can be further concentrated to 87.1% and about 70% respectively.

采用分步降温的尿素包合法富集多不饱和脂肪酸,在经济酯脲比1:15的条件下,经一次脲包富集后,紫苏油中的ALA含量由62.45%提高至85.68%,鱼油中EPA和DHA总含量由30.05%提高至67.32%;随着尿素用量的增加,ALA含量可高达87.16%,EPA和DHA总含量可超过70%。

The test areaswas 480.24 m2.The results were as follows:the excessive amount of organicfertilizer or urea was the cause of plant spindling and falling over. Application ofdifferent fertilizers for organic fertilizer≤15 000 kg/hm2,urea≤150 kg/hm2,KCl≤225kg/hm2,fused calcium magneium phosphate≤450 kg/hm2,have theapparent effects on increasing the individual plant productivity,the weight of one hundredgranule and the weight of one hundred kernel.Organic fertilizer and urea showed moreapparently effects.

结果表明,有机肥或尿素的过量使用是植株徒长倒伏的主要因子;增施:有机肥≤15000 kg/hm2,尿素≤150 kg/hm2,KCl≤225 kg/hm2,钙镁磷肥≤450kg/hm2,都能明显提高花生的单株生产力、百果重和百仁重,其中有机肥和尿素的效果极明显。

For example, blood urea concentration as an accurate measure of kidney function and the concept of extrarenal azotemia were first reported at that time.19 It was then believed that the kidney expended considerable energy to excrete urea into the urine and that moderate restriction of protein was beneficial to human patients as contended by Addis.20 This concept fell out of favor in human medicine when it was realized that renal work is tied closely to active sodium reabsorption and that urea is passively handled.

举例来说,血中尿素浓度依照肾脏功能的准确测量和肾外尿毒症的概念都是在当时第一次揭露。19而那时大家相信肾脏花费相当多能量以分泌尿素到尿液里,然而Addis主张适当限制蛋白质的摄取,是有益於患者。20这个观点赞同人类服用的药物上使用,当肾功能被积极钠紧密连接重新吸收,而尿素却是处於被动控制。

Results The purity and following treatment of inclusion bodies of CPB were influenced by the culturing temperature after the inducement with IPTG. The solubility of inclusion bodies of rCPB in 10mol/L urea was 2~3 times higher than in 8 mol/L urea, and the renature solution with 0.75%β-ME in 10mol/L urea could increase the solubility of inclusion bodies of rCPB, comparing with that in 10mol/L urea simply.

结果诱导后生长温度不同对rCPB包涵体的纯度及后期处理均有影响;rCPB包涵体在10 mol/L尿素溶液中的溶解度比在8 mol/L 尿素溶液中提高2~3倍;添加0.75%β-巯基乙醇能显著改善rCPB包涵体的溶解效果。

Pot and field experiments were conducted to study the response to application rate of urea labeled with ~(15)N combined with compound nitrogen fertilizer synergists in the growth,yield,uptake and utilization rate of urea of maize,In pot experiment,the standard urea application rate is 120mg/perpot;in field experiment,the standard urea application rate is 157.5kg/hm~2. Maize with ~(15)N-lurea.

用15N-尿素进行盆栽和田间试验(盆栽试验尿素标准用量为120mg/盆,田间试验尿素标准用量为157.5kg/hm2),研究了复合氮肥增效剂用量、尿素减量与复合氮肥增效剂配施对玉米生长、籽粒产量和氮素吸收利用的影响。

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