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Now the Company has and Japan PET raw materials, liquid crystal protective film manufacturers set up good relations of cooperation, the introduction of Japan's raw materials and advanced production technology, in Guangzhou City, Tianhe Industrial Park, set up factories in cutting production in Japan PET protective film LCD Products.

现本公司已和日本PET液晶保护膜原材料生产厂家建立良好的合作关系，引进日本的原材料和先进的生产工艺，在广州市天河工业园设厂切割生产日本PET液晶保护膜产品。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

Cunningham's schedule opened up, so if you'd like, we can move up your surgery to Wednesday.

还有， Cunningham 医生有时间了，如果你愿意的话，我们可以把你的手术时间提前到星期三。

Next, slice up a banana peel into small pieces, put them in the bottle, add 1 cup cold water, and shake it up.

接着，将一根香蕉切成小片状，将香蕉片放入瓶中，加一杯凉水，摇晃均匀。

Convenience items are up, too, including an available eight-inch navigation screen, and auxiliary and USB inputs for the stereo, while an electronic parking brake (with a hill-holder function) frees up important cupholder space in the center console.

方便项目最多，也包括现有8英寸导航屏幕，及辅助和USB立体声输入，而电子驻车制动腾出了空间重要cupholder在中心控制台。

Actually curassow discovers, a lot of conduct propaganda methods in the net that clean out treasure, do not suit everybody really, cite two case casually, write the article of experience introduction, this is not to have strength of certain written language, draw up very hard come, and should accumulate much experience to just go, and the top that otherwise breaks is stuck, actually the article draws up coming is very effectual, the at least a lot of friends' can intuitionistic struggling course that goes facing you, such is opposite for you, also can make a lot of good friends actually, if potential client saw your article, can very fast go knowing you, and trust you.

其实大鸟发现，很多在淘宝网的宣传方法，的确不适合所有人，随便举两个例子，写经验介绍的文章，这个不是有一定文字功底，很难写出来，而且要积累大量的经验才行，而且要不断的顶贴，其实文章写出来是很有效果的，至少很多朋友会直观的去面对你的奋斗历程，这样对你来说，其实也可以交到很多好朋友，假如潜在客户看到了你的文章，可以很快的去熟悉你，并且信任你。

"When the Cure were in Chicago for their Curiosa Tour, Robert had come by my house and we sat up all night playing guitars and shooting the whatever and so the idea came up about stuff we wanted to work on together," Corgan explained.

"当Cure乐队为了他们的Curiosa巡演来到芝加哥的时候，Robert到我家弹了一晚上的吉他，还瞎搅和，然后我们便产生了一起合作的念头。"

When I grow up I'm going to have a couch in my house. A red couch where I can curl up and read.

我长大后要在我的房子里摆一个沙发，要红色的，我好蜷在沙发上看书。

I thought of, the painstakingly make up that day before leave: I stealthily used my sister's lash curler, I ziroed in on clamping my eyewinker, but they were more persistent than me, never gave them up, and still clung on my eyelids forcibly, refusing to help my luxury date.

我想起，我出门前刻意的修饰，我偷偷地使用了姐姐的睫毛卷，我拼命的夹，但是我的睫毛比我更加倔强，它始终不肯屈服，还是那样有气无力地搭在我低垂的眼皮上，它甚至不肯成全我的一次奢侈的约会。

Up for. And dorm life was terrific. I lived up at Radcliffe, in Currier House.

哈佛的课外生活也很棒，我在Radcliffe过着逍遥自在的日子。

For you say, My doctrine is pure, And I am clean in Your eyes.

11：4 你说，我的道理纯净，我在你眼前清洁。

The method takes make water sedimentary in patient of be in hospital of leucocyte grow in quantity 30, according to clean middle make water bacterioscopy result, distinguish the patient to affect group and blame infection group, pathology of puncture of farther travel kidney analyses blame infection form.

方法取尿沉渣中白细胞增多住院患者30例，根据清洁中段尿细菌检查结果，将患者区分为感染组和非感染组，非感染组进一步行肾穿刺病理分析。