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Results (1) The total RNA, reverse transcription product and fluorescence labeled cRNA were all of high quality;(2) There were 420 up-regulated genes and 552 down-regulated genes among 2-fold DEGs, including DKK1 (dickkopf homolog 1) which was 5-fold up-regulated;(3) The results of real time RT-PCR, using β-actin as an internal control, showed that the 2-ΔCt values of DKK1 in HCC, PCT and NLT were 0.089 504, 0.007 65 and 0.000 631 respectively.

结果 （1）配对组织总RNA质量高，反转录cRNA及荧光标记质量好；（2）2倍差异表达基因中，上调基因共420个，下调基因共552个，其中包括5倍上调基因DKK1；（3）以β-肌动蛋白为内对照的实时逆转录聚合酶链反应结果提示，DKK1在癌、癌旁和正常肝组织中的2-ΔCt值分别为0.089504、0.00765和

A lot of people certificated the transgenic crops, simultaneously they also considered that the existence and development of target gene in natural environment probably change the species constitutes in nature ecological environment, pick up the deracination speed of many endangered species, make up the potential threaten and influence on the biodiversity.

许多人在认可转基因作物的同时，也认为，目的基因在自然环境中的存在和发展可能会改变生态环境中的物种组成，加快许多濒危物种的灭绝速度，对生物多样性构成潜在威胁和影响。

Despite the tough odds, Infiniti hasn't given up completely on its staid QX56 and has optioned up the SUV for another year of sales.

尽管艰难的赔率，英菲尼迪一直没有完全放弃其稳重QX56和optioned了SUV的一年的销售。

That clogs up the emails of those of us who are on dial-up for at least 20 minutes.

这对我们阻塞了谁的拨号至少20分钟，是那些电子邮件。

The purposes of education that he prescribe are diplex: blending the nationalism and individualism, causing up growth of the personal body and progress of moral and intellect, bringing up the personal attainment, body and mind"s ability, attitude and temperament, dominator and assistant of skill and knowledge, which needed by carring out the citizen"s responsibility.

他为人所规定的这一教育的目的是双重的：将国家主义教育和个人主义教育融为一体，促成个人的身体发育以及道德和理智的进步，造就具有履行国家公民职责所需要的个人素养、身心能力、态度和性情、技巧和知识的统治者和辅助者等级。

This paper,in which the blow-up rate of the blowup solutions is evaluated with appropriate hypotheses,considers the blow-up rate of a degenerate reaction-diffusion system with homogeneous Dirichlet boundary conditions.

文中考虑了一类退化反应扩散系统在齐次D irichlet边界条件下的爆破速率。在适当的假设条件下，得到了爆破解的爆破速率估计

On reaching Smolensk on the evening of the 4th of August, Alpatitch put up where he had been in the habit of putting up for the last thirty years, at a tavern kept by a former house-porter, Ferapontov, beyond the Dnieper in the Gatchensky quarter.

八月四日傍晚，阿尔帕特奇到达斯摩棱斯克，住宿在德聂伯河对岸的加钦斯克郊区，费拉蓬托夫的旅店里，三十年来他在这里住习惯了。

When you employ a dollar cost averaging approach, you end up buying more shares of stock when the market is down and less shares when it's up.

如果你采用平均成本法，无非就要在市场下跌时买入股份，市场上扬时出手一部分股份。

As the senator and his wife, Cindy, boarded a plane in Phoenix bound for Dayton, Ohio, reporters shouted a barrage of questions at the senator about whether he'd made up his mind. Sen. McCain flashed a double thumbs-up and boarded the plane.

麦凯恩和夫人辛迪登上从菲尼克斯飞往俄亥俄州代顿的飞机时，记者向麦凯恩抛出了诸多问题询问他是否确定竞选搭档，而麦凯恩只是竖起双手大拇指，没有回答问题就匆匆登机。

Bloggs shook him roughly," Wake up, you dozy bastard, wake up!"

布洛格斯用力摇着他说："醒醒，快醒醒，你这瞌睡虫！"

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。