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The bioinformatics analysis showed that the gene had the highest homology with the gene of Penicillium janthinellum, which reached up to 76%. The first 26 amino acids might be a signal peptide sequence with the hydrophobicity of up to 2.63. The CBHⅠ gene consisted of the catalytic domain, convergence zone and cellulose-binding domain and its tertiary structure was mainly composed of 3-sheets.

分别扩增到鹅源草酸青霉F67纤维素酶CBHⅠ基因片段A(EU574736)、5'端序列B1(EU603295)、3'端序列B2(EU652768)及全长基因C(EU727171)，并成功构建真核表达载体pPIC9K-CBHⅠ；生物信息学分析表明，该基因蛋白序列与微紫青霉氨基酸序列同源性最高，达76%，前26个氨基酸为信号肽序列，疏水性可达2.63，由催化功能域、衔接区和真菌性纤维素结合域构成，其三级结构主要为β-sheet。

Sepals up to 7 mm; corolla tube up to 1 cm.

萼片可达7毫米；花冠筒可达1 厘米 27

He had gotten right up to the machine, and I crept up closer to see him better.

他正确地已经赶上机器，和我爬在比较靠近的上面见他更多。

Not wanting to wake her up, he crept quietly up to his bedroom .

他不想惊醒她，于是蹑手蹑脚地悄悄走到自己的卧室。

The results showed that behavior of the smog and TSP are in accordance to the formula of dynamics, changes of cresol mixed isomers' density conform with exponential function. With air disinfecting in indoor with smog, the killing percentage of Staphylococcus auras are up to 99.92% during 30 min, and that of natural bacteria is up to 99.66% during 120min, and the sm...

结果表明，烟雾及其有效成份在空气中按一定的规律沉降；并且在 30min作用时间内能够有效地杀灭空气中 99.92 %的金黄色葡萄球菌；在 12 0min内空气自然菌的平均杀灭率可达 99.66%，烟雾对室内空气环境具有一定的净化作用。

In the beginning, his meals were epic, up to 45 minutes: He'd sip daintily, and take breaks, and sometimes nod off; I'd pinch his toes or scratch his head to wake him up again, in the hopes that he'd fill his belly enough to give us both a few more hours of sleep without interruption.

一开始，他的饮食如一个史诗一般，多达45分钟：他可口地啜饮着，并休息一会，有时甚至在点头；我想捏一下他脚趾或刮他的头部叫醒他一次，希望能填饱他的肚子，足以让我们享受一个几个小时都不会中断的睡眠。

For call up the Chinese people We need dial three times, total 27 numbers, then dail-up to the receiver number, but on the dialogist's mobile phone the number is not pressented, or displaid "anonymith calling", some receivers will not like to connect it, because of anonymith…..

在芝加哥给咱们中国打电话先要分三次一共拨27个号码，然后才能拨对方电话号码，拨通后，对方手机上不显示芝加哥的电话号码，或者只显示为"匿名电话"，对方有的人会因为这个"匿名电话"而不愿接呢

General description: Fast growing deciduous hard woody tree; does not stand up well to wind; medium-sized; dicotyledon; angiosperm; with umbrella-shaped crown and drooping branches; up to 6-12 m tall; prefers sunny and sheltered position.

概述： 生长迅速的硬木质落叶乔木树。抗风力弱。中等大小，双子叶植物，属被子植物门。有伞形树冠及下垂之枝条。高6至12米。适宜在向阳光及不当风的地点生长。

You can safely leave food to cool on the counter for up to an hour after cooking, Snyder says. Or divvy up hot food into smaller containers and then refrigerate — it'll cool faster.

做完饭，你可以放心地把食物放在柜子上搁一个小时，施耐德说，或者你把食物分放到更小的容器里，然后冷冻－－那样能凉得更快。

SELF-PROPELLED TRUCKS UP TO AND INCLUDING 10000 KG CAPACITY AND INDUSTRIAL TRACTORS WITH A DRAWBAR PULL UP TO AND INCLUDING

达到和包括10000千克容量的自推动式卡车和带有一达到和包括20000N的拖斗的工业拖车。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。