up to 5

We plan to get an one-up place in the nation for our products, asuring to ahead of other products for 5 years.

使新奇生的产品处于国内领先的地位，确保比其他厂商领先5年

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Base on HPLC method, we select 5 peak to set up fingerprint in order to identify Desmodium sequax and Phyllodium pulchellum.

另外，在建构指纹图谱方面，确定选取5个波峰来比对波叶山蚂蝗和排钱树，确实可作为鉴定上的参考。

Fertilizing date presented obvious effects on root growth and distribution. Fertilization after watering was more primitive to the root in up-soil layers than before watering. Fertilization of Ca-Mg was positive to the increase of root in 5-15cm soil layer, but negative to that in the surface layer. Both fertilization date and Ca-Mg fertilizer affected root vigor obviously.

施肥时期对根系也具有明显的影响，与灌水前施肥相比，灌水后施肥有利于促进根系在上层土壤中的生长和分布；钙镁肥的施用能增加5~15cm土层根重，但对表层根的生长不利，施肥时期和钙镁肥施用与否对根系活力的影响也较大。

As we have learned, the mobile spot April 5 is close 1000 audiences are sign up of one's own accord by the player participate in, the round member of vermicelli made from bean starch of current and a lot of star also was become most ripsnorter group, in joining each game below division of perfect and spatio-temporal company in succession " mad experience ", do one's best obtains to enter quota of people, according to reporter understanding, the star that mobile spot abounds arrangement and player are interactive link, no matter star, vermicelli made from bean starch still is a player,believe, will experience unprecedented joy in grand ceremony spot.

据了解，4月5日的活动现场近千名观众是由玩家自愿报名参与的，当前诸多明星的粉丝团成员也成了最为活跃的人群，已经纷纷加入了完美时空公司旗下的各个游戏中"疯狂体验"，力求获得一个入场名额，据记者了解，活动现场将安排丰富的明星和玩家互动环节，相信无论明星、粉丝还是玩家，都将在盛典现场体验到前所未有的欢乐。

However, in the sea with the letter and determined to win every time Montoya举牌always bid less than 5 seconds to follow up quickly, so very saddened by spectators.

但是，在海中的文字和决心赢得每一次总是蒙托亚举牌竞标不到5秒的后续行动迅速，因此非常难过观众。

In 2003, a piece of strange meat without the bones and organs was drifted up the nearby seabeach of the Monte Port in Chile. This piece of meat is 12 meters in length and 13 tons in weight. 80 years ago, people found a piece of strange meat of 5 to 7 tons on the seabeach of Florida, U.S.A. When people tried to cut it, it was so flexible, tough and tensile that it broke actually the knife.

2003年，一种没有骨骼和器官的怪肉被冲上智利蒙特港附近的海滩，长12米，重13吨。80年前，人们在美国佛罗里达州海滩也发现了一块重5到7吨的怪肉，当人们试图将它割开时，它坚韧而富有弹性的外皮竟把刀片弄断了。

The results showed that the frequency of germination were able to be increased by keeping seed vessel, culturing seeds with radicle up and pre-treating seeds in 5.0mg/L GA3 solution for 12h. The frequency of explant-browning was reduced by adding AC 0.5mg/L to induction media and the vigor of plantlets was promoted by the AC-adding. The propagating coefficient could be improved and the frequency of bud-vitrifration increased by adding 150mg/L coconut water.

结果表明发芽时保留种皮、接种时胚根向上对蚕豆种子的萌发有着至关重要的影响，用5.0mg/L GA3溶液浸种12h有利于种子萌发；培养基中添加0.5g/L AC能有效抑制外植体的褐化，并能使植株生长健壮；培养基中添加150mg/L椰汁能提高繁殖系数，有效抑制玻璃苗的发生。

And There was no serious complication;②In the course ofinterventional therapy, Direct portal vein angiography demonstrated vena coronaria ventriculi(100%)andgastricveins(65.26%)andvenagastricaposterior38.43%, Angiography demonstrated venacoronariaventriculi communicate esophagus varicose veins, gastric veins and vena gastrica posterior communicategastric varicose veins. vena coronaria ventriculi had only a small percentage of double vein, about30.57%. The sites of vena coronaria ventriculi arising from the portal vein, splenic vein, portosplenic junction, were found in 52.06%、27.39%、20.55% respectively.③12 extrahepaticprotosystemic shunts were found in these patients. Include gastro-nephrosshuntof 3 cases, 7 caseswere splenetic- nephros shunt and 2 cases shown recanalization of umbilical vein .④The averageportal pressure before and after the procedure were 3.87±1.82kPa and 3.64±1.14kPa in 73patients, but to the time of rebleeding, it was 3.96±0.23kPa in the 11 cases.⑤There werethree kinds of variceal outcome: disappearance (54,low degree (19).⑥Spearman logisticanalyse and ANOVAtest shown liver function class, variceal degree of the splenic necrosis area,the blood direction in portal vein before operation and remain small collateral routes were thesignificant factors concerning outcome of varices.⑦The bleeding volume and portalhypertensive gastropathy are main risk factors of rebleeding.⑧The course of livercirrhosis is the risk factor of survival and extrahepatic portosystemic shunt , fine varices are thebeneficial factors to survival.⑨During all cases'followed-up data, the 1, 2, 3, 4, 5 yearcumulative survival rates and rebleeding rates were 17.81%, 28.77%, 38.36%, 43.84%, 47.95%and93.15%,91.78%,86.30%,83.56%,80.82%respectively. Conclusion The interventional disconnection treatment for liver cirrhosis and portalhypertension was designed suitability. It rapidlycontrol bleeding,butpressure of portal vein was notobvious high, perfusion was not low .it was compared with surgery therapeutic that interventionaldisconnection treatment was safe and had a significant clinical effect to hemorrhage and preventfrom rebleeding.

结果：①术后一过性发热62例(84.9%)，腹痛腹胀48例(65.8%)是介入断流术常见的并发症，未发生严重并发症；②门静脉造影显示胃冠状静脉、胃短静脉和胃后静脉的曲张分流的出现率是100%、65.26%和38.34%；显示食管静脉曲张主要由胃冠状静脉供血，胃静脉曲张主要由胃短静脉和胃后静脉供血；胃冠状静脉大多数为单支，少数为双支，其双支的出现率分别为30.57%；胃冠状静脉开口于门静脉主干的为52.06%，开口于脾静脉主干的为27.39%和开口于门脾静脉交汇处的为20.55%；③发现胃肾分流3例，脾肾分流7例、腹膜后门腔静脉分流2例，以及CTA检查发现脐静脉开放者2例；④73例患者介入断流术前和术后平均自由门静脉压力分别为3.87±1.82kpa和3.64±1.14kpa，前后比较存在显著性差异；11例再次介入手术患者的术前、术后和复发后的自由门静脉压力分别为4.02±0.24kpa、3.82±0.25kpa和3.93±0.23kpa ，前后比较发现首次术前与术后存在显著性差异，首次术前和复发出血术前门静脉压力比较无显著性差异；⑤介入术后复查曲张静脉转归基本消失54例，轻度19例；⑥Spearman相关分析和Logistic多因素回归分析，肝功能分级、静脉曲张程度、门脉血流方向和残存小侧支四个因素对曲张静脉转归有影响；Spearman相关分析和Logistic多因素回归分析门脉高压性胃病和出血量等因素对复发出血时间有影响；⑦COX回归分析，门体分流和曲张静脉转归两个因素对术后生存有影响；⑧术后随访6-70月，1、2、3、4、5年的累计复发出血率和累计生存率分别为17.81%、28.77%、38.36%、43.84%、47.95%和93.15%、91.78%、86.30%、83.56%、80.82%；结论：介入断流术治疗门脉高压食管胃底静脉曲张有独特的优点，可以快速直接控制曲张静脉出血而门静脉压力无显著增高，保证了肝脏灌注；与外科分流术相比适应证广、损伤轻、术后恢复快，不易遗漏曲张静脉；肝功能分级、曲张静脉程度、门脉血流方向和残存侧支血管对食管胃曲张静脉转归有影响；门脉高压性胃病对复发出血时间有影响；门体分流和曲张静脉转归对生存时间有影响。

So water expends every stere 3.7 yuan, want 5.6 yuan now; Charge of electricity also went up, calculate come down to want to compare former about one times taller.

原来每立方米水费3.70元，现在要5.60元；电费也上去了，算下来要比原先大约高一倍。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。