up to 5

The sowing depth of spring sowing (through the stratification of damp sand) was optimum at 5~6cm, and the seedling emergence rate was up to 98.5%. Chop, chip, abdomen grafting and bud grafting with xylem went well in early and middle March and the suitable slacking time was from late July to early August.

春播播种深度以5~6cm为佳，出苗率达98.5%；劈接、切接、腹接、带木质部芽接宜在3月上中旬进行，适宜的解绑时间是7月下旬~8月上旬。

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

objective to evaluate the value of infrared thermography in the diagnosis of injuries in such soft tissues as neck,shoulder,waist,leg,elbow and wrist.methods infrared thermograph (type wp-95) was used to do general physical check up for 221 young soldiers with a regular military training history ranging from 1 to 5 years,whose infrared thermograms were then compared with those of 100 new recruits without any military training experience.results there were abnormal thermograms on 180 (81.45%) of the 221 cases and 6 (6%) of the 100 new recruits （p.05.all of the 186 cases with abnormal thermograms were x-rayed with only 28 cases(i.e.15.05%) abnormal.there was a distinct difference between the two methods of physical check-up (p.05).conclusion infrared thermography manifests distinctive abnormal thermograms of soft tissue injuries suffered from military training,providing definite diagnostic message for clinic.to utilize infrared thermography correctly is of vital importance in ensuring the health of servicemen.

目的 探讨软组织损伤颈、肩、腰、腿、肘、腕红外热像图的诊断价值。方法应用wp-95型红外热像仪，对221例常规军事训练1～5年的年轻干部及战士进行红外热像仪的全身检查，并与刚入伍未参加训练的新兵100例进行对照。结果 221例经红外热像图检查发现颈、肩、腰、肘、腕出现异常热像图的有180例，占81.45%；而100例新战士仅有6例颈、肩、腰、肘、腕出现异常热像图，占6.00%，两者比较差异有显著性（p＜0.05）。186例异常热像图受检者全部做x线摄片检查，x线摄片异常者仅28例，占15.05%，两者检查比较差异有显著性（p.05）。结论红外热像图对军事训练所致软组织损伤有其独特的异常热像图，为临床提供明确的诊断信息，正确的使用红外热像图，对保证部队广大指战员的健康水平有着重要作用。

Asynchronous loading of control parameters · Rapid (25ns per pixel) rotation, warping, panning, and scaling of images · Three-dimensional image addressing capability · General third-order polynomial transformations in two dimensions on-chip · Three-dimensional transformation of up to order 1.5 also supported · Flexible, user-congurable pixel datapath timing structure · Static convolutional ltering of up to 16 x 16 Pixel, 256 x 256 pixel or 256 x 256 x 256 pixel windows · User-selectable source image subpixel resolution of 2-8 to 2-16 · Pin-compatible upgrade to TMC2302 · 24-bit (optional 36-bit) positioning precision within the source image space, 48-bit internal precision · Low power CMOS process · Available in a 120-pin Plastic Pin Grid Array and 120-lead Metric Quad Flat Pack

1第1页，本页显示记录1-11，共11条记录分1页显示·异步控制参数加载·快速（25ns的每个像素）旋转，弯曲，平移，缩放和图像·三维图像处理能力·一般二维三阶多项式变换片·三高达1.5三维变换命令也支持·灵活，用户可配置的数据通道的静态时序结构·高达16 × 16像素滤波卷积像素，256 × 256像素或256 × 256 × 256像素（3通）窗口·用户可选的源图像亚像素分辨率的2-8 2-16·引脚兼容的升级TMC2302·24位（可选36位）的定位源图像的空间内，48位内部高精度·低功耗CMOS精密加工·提供120引脚塑料针栅阵列和120引脚公制四方扁平封装

Spikelets with 1 floret (very rarely 2), rachilla disarticulating above glumes, with penicillate extension lying against the palea; glumes subequal, usually slightly longer than the floret, rarely slightly shorter, membranous, 1–3-veined, apex acute or acuminate; floret callus bearded, hairs usually 1/3 as long up to about equaling floret; lemma thinly to firmly membranous,(3–)5-veined, dorsally awned or awnless, apex erose, denticulate or 2–4-toothed; awn geniculate or straight, its position varying from near base to near apex, usually inconspicuous, up to twice length of lemma but occasionally reduced to a subapical mucro or absent; palea 2/3 as long to subequaling lemma.

小穗具1小花(极少2)，高于颖片，具具毛撮的扩展对内稃躺脱节的小穗轴；通常，近等长的颖片稍长于那些小花，很少稍短，膜质，脉，先端锐尖或渐尖；作为长的可达的通常的公然反对的小花结茧，头发1/3大约等于小花；稀疏外稃到坚定膜，(3-)5－脉，背具芒或者啮蚀状的顶，或者2-4齿状的具小齿，awnless；芒膝曲或者直，作为近相等外稃的长时期的内稃2/3。

A process for reducing nitryl with unstable guanyl on para-position of benzene ring into azyl features that active metal reacts on organic acid and solvent at ordinary temp. and pressure to reduce N-(2 or 3-M, 5 or 6-M',4-nitrophenyl)-N',N'-dimethyl acetamidine into N-(2 or 3-m, 5 or 6-M', 4-aminophenyl)-N',N'-dimethyl acetamidine, where M=H,-R,-OR,-COOR (R=H, C1-C6); and M'=H,-R,-OR,-COOR (R=H, C1-C6). Its advantages include reduced by-reaction and high reducting rate up to 80%-95%.

本发明是将苯环上对位带有不稳定脒基的硝基还原为氨基的方法，采用活泼金属，如：锌或锡，与有机酸和适当的溶剂，在常温、常压下发生反应，将N－（2或3－M，5或6－M＇，4－硝基苯基）－N＇，N＇－二甲基乙脒还原为N－（2或3－M，5或6－M＇，4－氨基苯基）－N＇，N＇－二甲基乙脒，其中M＝H，－R，－OR，－COOR（R＝H，C1－C6），M＇＝H，－R，－OR，－COOR（R＝H，C1－C6），减少了副反应，使还原收率达到80％以上，最高可达95％以上，更适用于工业化生产。

The conclusions were as follows: theνO-H andνC=O IR absorption maxima shifted towards higher wave numbers after imprinting 17β-estradiol on TFMAA-co-TRIM copolymer with the red shift of theνO-H groups being apparent after precipitation polymerization for 16h while that forνC=O groups being observed after polymerization for 24h. A strong interaction between TFMAA and 17β-estradiol was confirmed by the high selectivity for 17β-estradiol, as indicted by the values of the separation factor of isomers of 17β-estradiol/17α-estradiol (2.28) and the imprinted factor (3.01). Particle diameter of TFMAA-co-TRIM polymer was between 300 nm and 1.5μm, which suited well for solid phase sorbent throughout at low column pressure. The recognition of imprinting TFMAA-co-TRIM polymer for sterol molecules was driven by enthalpy eluted with acetronitile, and low temperature was in favor of the separation of sterol structure analogues on imprinting column. TFMAA-co-TRIM polymer possessed of determinate anti-heat stability, with melting point beginning at 255.84℃, Tp=257.40℃, control TFMAA-co-TRIM polymer and imprinting TFMAA-co-TRIM polymer were respectively decompounded at 267.79℃and 343.11℃, and solid micro-extraction noddle prepared by the special polymerization also showed definite recognition for 17β-estradiol by GC/MS detecting at 270℃. By selection of various washing and elution solvents, elution reagents of close polymerization system were of more advantage in template molecules retention and recognition on molecularly imprinted solid phase extraction column. At a certain extent, progesterone, 17α-estradiol, 17β-estradiol and 4-androstene-3, 17-dione could be intercepted on the MISPE column. Especially, MISPE had high selectivity for progesterone, and imprinting TFMAA-co-TRIM polymer could achieve adsorption balance within 50 min by absorption kinetics test for 17β-estradiol. However, MISPE column showed better selectivity and enrichment property for 17β-estradiol than C18 and CSPE columns according to the data from HPLC and GC/MS analyses. Recovery of 17β-estradiol on MISPE column was up to 85.5% while when prime extracting solution of milk powder was sampled, the recovery of CSPE and C18 columns were 43.7% and 30.7%, respectively.

通过研究阐明：紫外聚合产物TFMAA-co-TRIM中的νO-H振动吸收峰在聚合16h后红移，νC=O振动吸收峰在聚合24h后红移；TFMAA-co-TRIM对雌二醇异构体的印迹因子达到了3.01，α为2.28，优于其它功能单体参与得到的聚合物识别特性；TFMAA-co-TRIM聚合物粒径介于300 nm至1.5μm之间，作为色谱固定相具有良好的通量和低的柱压；在乙腈流动相中，TFMAA-co-TRIM分子印迹固定相的分离过程主要被焓驱动，低温有利于分子印迹固定相分离甾醇结构类似物；TFMAA-co-TRIM分子印迹聚合物在255.84℃时开始熔融，Tp=257.40℃，聚合物CP的降解温度在267.79℃，MIP降解温度在343.11℃，制备的固相微萃取头初步经GC/MS 270℃的耐热性测定；不同的洗提溶剂筛选证明了选择接近聚合溶剂的洗脱体系更有利于MISPE发挥识别效应；分子印迹固相萃取柱对孕酮、17α-雌二醇、17β-雌二醇和雄烯二酮都具有不同程度的保留特性，可作为此类化合物的吸附材料，特别对孕酮强保留的富集特性，可作为孕酮的选择性识别吸附剂，其分子印迹聚合物对17β-雌二醇的吸附动力学测定显示在50 min内基本达到吸附平衡，具有作为传感器核心敏感材料的潜力；对比萃取奶粉中17β-雌二醇性能，MISPE柱比非分子印迹固相萃取柱和C18柱具有更高的保留，回收率依次为85.5%，43.7%和30.7%。

With wingspans of up to 72 inches and up to 24 inches in length and a weight of almost 5 pounds, the osprey is a wonderful addition to Montana's rich abundance of bird life.

与wingspans多达72英寸，长达24英寸的长度和重量将近5磅，鱼鹰是一个美好的除了蒙大拿丰富的丰富的鸟类生活。

2 BJP direct toxicity on TEC NRK52E cell were subcultured on a 24-well plate (3×10〓/ml/well) and exposed to BJP κ or BJP λ in concentration 1, 5, 10, 20mg/ml for up to 24h, then the media was changed, and 〓H-thymidine was added for incubation for 18h at 37℃, the corporation was terminated, Acid-insoluble DNA was precipitated by adding 5% trichloroacetic acid radioactivity in the extracts was determined in scintillation counter.

细胞增殖的影响：将TEC株NRK52E以3×10〓/ml/孔分别加入24孔培养板，分别加入BJPκ与BJPλ（浓度1、5、10、20mg/ml）共同培养，均设3个复孔，24小时后加入〓H-TdR（1μCi/孔）在5%CO〓37℃孵育18小时，终止反应，加胰酶脱壁半小时后移到普通干燥试管，多头细胞收集器抽滤，用5%三氯醋酸，无水乙醇分别冲洗，烤干，冷却后加入闪烁液，放入液体闪烁计数仪测定放射性。

New box design with durable 0.5 inch (13 mm) paving skin Vertically-adjusting mold mount for precise leveling of mold to machine Telescoping end sections with 12 inches (305 mm) of width variation on each side are available Standard paving depth is zero to 24 inches (610 mm)Edge slump adjustment Hydraulic Vertical Hinged Sideplates, self-contained inside the mold for track clearance Split, pressure-compensated sideplates Folding side plate wings for transporting without removing Pivoting mold mounting beam to eliminate stress points Self-supported TA is hydraulically driven with 3.5 inch (89 mm) ACME screws for up to a six inch (152 mm) crown Front and rear top T-bar on mold for attaching accessories and structural integrity Inserts are bolted together with front and rear alignment pins for easy mold assembly New vibrator mounting tube attaches to T-bar on mold – Vertical vibrator lift – Rear lubrication system with grease zerks accessible from the work bridge New strike-off – Mounted on paver frame T-rail, independent of the mold – New modular design with wedge lock system for ease in changing widths – 10 inches (254 mm) of hydraulic height adjustment – Hydraulic crown adjustment Spreader plow mounted to paver frame T-rail, optional auger available Tamper bar optional

与耐久性的新的箱子设计铺精密水准测量的0.5英寸(13 mm)皮肤垂直调整的模子登上模子用机器制造挤撞与12英寸(305 mm)的末端部分在每边的宽度变异是可利用的标准铺的深度是零对24英寸(610 mm)边缘暴跌调整水力垂直的取决于的Sideplates，独立性在轨道清除分裂的模子里面，折叠运输的pressure-compensated sideplates旁边板材翼没有去除在轴上旋转的模子架置射线消灭重音点Self-supported TA水力驾驶与3.5英寸(89 mm)由六英寸(152 mm)冠决定的尖端螺丝在前后冠上在模子的T酒吧附有的辅助部件并且结构正直插入物与容易的模子汇编新的振荡器架置管随员T酒吧的定位销一起在前后被闩上在模子–垂直的振荡器推力与油膏zerks的–后方润滑系统容易接近从新工作的桥梁触击–在摊铺机框架足迹，模子的独立登上了与楔子锁系统的–新的模块设计在改变的宽度的舒适的– 10英寸(254 mm)水力高低调整–水力冠调整分布器犁登上了对摊铺机框架足迹，任意任意木钻可利用的堵塞器的酒吧

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。