查询词典 uninucleate

Based on extrapolated organ shape criteria,we select ears that are in uninucleate period,carry out pretreatment in a 6~8℃ low-temperature condition for about 12 days, and callus induction rate can be enhanced in this way.② 1~2mm diameter should be the appropriate size for translocated callus,and plants grow to 4cm breeding for mature plants should be conducted in a timely manner.③ Get well down with sterilization,reduce contamination and strengthen greenhouse management,etc.

根据器官形态指标外推法选择花粉发育处于单核靠边期的幼穗，进行6~8℃低温预处理12d左右，可提高愈伤组织诱导率；②愈伤组织转移大小以直径1~2mm为宜，当绿苗长至4cm左右时及时进行壮苗培养；③做好接种材料、室内、人员的消毒和卫生工作，降低污染率，加强温室等的管理。

There are three copies of R fragment in the S mt-genome, and each copy contains two closely adjacent open reading frames: orf355 and orf77. Among these three copies two BamHI fragments were cloned, and the sequence analysis showed that cox1 and cox2 gene lies upstream of the R region in each fragment, respectively. However, they locate in different strands and head to head with R. In this study, we found that:(1) The expression of cox1 and cox2 is not affected by the transcription of R region;(2) The two copies of R region transcribe in large amount in the microspores, and produce transcripts of 1.6kb and 2.8kb, respectively, in the sterile microspores; However, the abundance of these two transcripts is reduced in the fertility restored microspores, realized through the mRNA decay, and this reduction occurs at the uninucleate microspore stage;(3) In the sterile microspores, the 5 terminus of the 1.6kb transcript contains a palindrome region that can fold into a stem-loop.

本研究发现：(1) cox1和cox2的转录没有受到R区转录的影响；(2)两个拷贝的R区在在不育花粉中高丰度表达，其转录本大小分别为1.6kb和2.8kb；而在育性恢复的花粉中，这两个转录本的丰度被大大降低，进一步分析表明这一结果是通过mRNA分子的降解途径来实现的，此降解过程发生在单核花粉期；(3)在不育花粉中，1.6kb转录本的5′末端具有一段可形成茎环结构的迴文对称序列，而在可育花粉中，其5′末端短缺了9个碱基；(4)在不育花粉和育性恢复的花粉中，R区转录本的加尾位点都集中位于一个3′茎环结构之后；(5)不管是在不育花粉还是育性恢复的花粉中，orf77区域内均在第52位和100位核苷酸处发生了不同频率的C向U的编辑，从而形成UGA终止密码；(6)不管是orf55-orf77的成熟转录本还是转录前体分子，都已被加上了Poly尾巴，表明已进入降解途径；在以上实验结果的基础上，本研究对S型CMS花粉育性的恢复机理进行了如下推测：在不育花粉和育性恢复的花粉中，orf77区域内均发生了终止编辑，即由RNA编辑产生了提前终止密码子。

GA(subscript 1+4) distribution in microspore mother cells, uninucleate and binucleate pollen, especially in nucleus, gradually increased with the anther development. Moreover, some GA(subscript 1+4) distributed in sperms.

短日照条件下花药可育，花粉母细胞、单核花粉和二核花粉内GA(下标 1+4)的分布数量逐浙增多，以细胞核最为明显；成熟花粉的精细胞内也有一定数量的GA(下标 1+4)分布。

The nucleus vegetative in nature derived from unequal or equal division of the uninucleate pollen in rye anthers cultured in vitro develops into pollen embryoids or calli.The green haploid plantlets are differentiated successfully.

离体培养黑麦花药，单核花粉可以通过不等分裂和均等分裂由营养性质的核发育成花粉胚状体或愈伤组织，并且最后形成了单倍体植株。

There were on unique different protein observed in flag leaf at different development stage; but unique different protein were found in young spike at uninucleate microspore stage and non-1B/ 1R type male sterile lines lack a 17kD polypeptide compared with maintainers; at binucleate microspore stage and non-1B/ 1R type male sterile lines increase 17kD polypeptide compared with maintainers.

利用SDS-PAGE凝胶电泳技术对采用丙酮沉淀法提取的K型，C49S型和YS型小麦不育系和保持系旗叶小穗发育的单核期和二核期中的可溶性蛋白进行比较后发现，非1B/1R类K型不育系732A在小穗发育的单核期比同时期相应保持系732B少1条分子量为17kD的多肽，在小穗发育的二核期比同时期相应保持系732B多1条分子量为17kD的多肽。

The results showed that the tapetum of Zhenshan 97B disrupted promptly at the late uninucleate pollen stage. Whereas, the tapetum of Zhenshan 97A disaggregated at the pollen mother cell stage, the nuclear membrane and the cell membrane disaggregated, the phenomena continued to the binucleate pollen stage.

研究发现保持系绒毡层细胞在单核花粉晚期才开始迅速解体，而不育系绒毡层细胞在花粉母细胞时期就开始出现核膜、细胞膜解体，此过程持续到二核花粉时期。

The observation showed that sterile plants and fertile plants in the pollen mother cell stage is not very different, when microspore mother cells of male sterile plants enter meiosis period, it could form normal tetrad cell , tetrad cell is normal in early development stage, In the uninucleate microspore stage , tapetal cells were vacuolization, the microspores were extruded to the middle by tapetal cells.

本研究利用石蜡切片法，结合光学显微镜技术对樱桃萝卜核质互作雄性不育系的不育株和对应保持系可育株的花药发育过程进行细胞形态学观察，观察结果表明：不育株与可育株在花粉母细胞时期差别不大，不育株的花药小孢子母细胞进入减数分裂期后，可以正常的形成四分体，四分体前期发育正常，而在单核小孢子期绒毡层细胞出现液泡化，绒毡层细胞向中间挤压小孢子。

Total anthers proteins were extracted from uninucleate and binucleate stage of-1376A and-1376B for IEF/SDS-PAGE.An higher repartability and better separatingprotein method from wheat anther for 2-DE was established.

以IEF／SDS-PAGE双向凝胶电泳技术，对小麦质核互作型雄性不育系-1376A及其保持系-1376B在花药发育的单核期、二核期蛋白质进行了差异蛋白质组学研究。

The results showed that about610 protein spots could be visualized on the two dimensional electrophoresis map bycoomassie brilliant blue staining within Mr 9.0～100.0kD and pH 4～7,and about 94 spotswere differential expressed between male-sterile line and its maintainer line by PDQuestsoftware.Among those differential proteins,about 49 spots and 40 spots were differentialexpressed between uninucleate anther stage and binucleate stage respectively;Meanwhilecomparing with the 2-DE maps of the total proteins from the binucleate anther stage anduninucleate stage,15 differential spots were detectable at male-sterile line;and 16 differentialspots were detectable at its maintainer line.

在分子量9.0～100.0 kD、等电点4～7线性范围内，可识别约610个蛋白质点，PDQuest软件分析结果表明：在单核期不育系和保持系之间存在40个差异蛋白质点，二核期两者共有49个差异点；不育系在单核期和二核期两者之间存在有15个差异点，保持系在两个不同时期有16个差异点；不育系与保持系间在表达量上的差异蛋白质点以及不育系与保持系间特异表达或特异缺失的蛋白质点很可能与小麦雄性不育有关。3。

In microgametogenesis, callose deposits in tapetal cells and begins to accumulate around microspore nucleus at the early microspore stage, callose deposition around microspore nucleus continues through uninucleate microspere stage.

小孢子早期，绒毡层细胞中积累胼胝质类物质，小孢子核周围也开始沉积胼胝质，逐渐形成完整胼胝质壁；小胞子晚期，绒毡层细胞开始解体，药室内壁细胞开始加厚，其加厚的物质为胼胝质类物质。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。